1998 Fiscal Year Final Research Report Summary
Identification of growth factors for neutronal and non-neutronal cells in the rat inner ear
Project/Area Number |
09671744
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Otorhinolaryngology
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Research Institution | Osaka University |
Principal Investigator |
DOI Katsumi Osaka University Medical School, Assistant Professor, 医学部, 講師 (40243224)
|
Co-Investigator(Kenkyū-buntansha) |
TAMURA Manabu Osaka University Medical School, Assistant Professor, 医学部, 講師 (50273644)
|
Project Period (FY) |
1997 – 1998
|
Keywords | cochlea / spiral ganglion cell / hair cell / HGF / c-met / RT-PCR / immunohistochemistry / sequence |
Research Abstract |
RT-PCR and DNA sequencing with rat cochlear cDNAs revealed expressions of mRNAs of hepatocyte growth factor (HGF) and its high-affinity receptor c-met in the rat cochlea. Immunohistochemistry with specific polyclonal antibodies raised against HGF and c-met demonstrated that HGF and c-met proteins were located within the organ of Corti (hair cells and neighboring supporting cells). HGF and c-met proteins were never detected in the Spiral ganglion cells of the normal rats. Kanamycin-deaf rats were developed by daily kanamycin intra-peritoneal injections after birth. Effects of systemic application of HGF on the survival rates of neuronal and non-neuronal cells were examined. After eight weeks, all hair cells were disappeared in the cochleae of both the HGF-treated and no-HGF deal rats. The number of surviving spiral ganglion cells of the HGF-treated rats was slightly larger than that of the no-HGF deaf rats, although there was no statistical significance between them. In the HGF-treated deaf rats, c-met proteins were detected in the spiral ganglion cells. These results suggest that HGF and its high affinity receptor c-met are active in the rat cochlea and that they could exert some protective effects on neuronal and non-neuronal cells there.
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