1998 Fiscal Year Final Research Report Summary
Pathogenesis of adhesive otitis media and study of mechanism of development into the pars tensa cholesteatoma
| Project/Area Number |
09671761
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | THE JIKEI UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
MORIYAMA Hiroshi THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY PROFESSOR AND CHAIRMAN, 医学部・耳鼻咽喉科, 教授 (60125036)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Tomihiko THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY,ASSISTANT, 医学部・耳鼻咽喉科, 助手 (30236880)
SHIWA Masanori THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY,ASSISTANT, 医学部・耳鼻咽喉科, 助手 (20235766)
MITANI Yukie THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY,ASSISTANT, 医学部・耳鼻咽喉科, 助手 (30233894)
KOJIMA Hiromi THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY,ASSISTANT, 医学部・耳鼻咽喉科, 助手 (60234762)
YAMAGUCHI Tatsuji THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY,LECTURER, 医学部・耳鼻咽喉科, 講師 (50182449)
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| Project Period (FY) |
1997 – 1998
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| Keywords | CHOLESTEATOMA / PKC / KGF / GAS EXCHANGE / ADHESIVE OTITIS MEDIA |
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| Research Abstract |
The in situ hybridization technique has been used to determine the mechanism of epithelial proliferation in cholesteatoma, and cultured cell lines, mainly of fibroblasts and keratinocytes, have been analyzed at the protein and gene level. Differentiation and apoptosis of the epithelial cells of cholesteatomas, particularly the effect of protein kinase C on differentiation, and the relationship between the interleukin converting enzyme family (ICE) and apoptosis are being investigated using molecular biological techniques.
The characteristics of terminal differentiation in epithelial cells have been analyzed by comparing the expression of differentiation markers for epithelium cells, especially focusing on protein kinase C (PKC), between cholesteatomatous epithelium, normal epithelium, and the epithelium invaded by psoriasis, a typical inflammatory hyperkeratosis.
The influence of KGF on differentiation markers or cell growth factors will also be analyzed in cultured cell lines to explain the mechanism of normal epithelial differentiation and cell growth inhibitors, and then to assess the possibility of conservative therapy for cholesteatoma.
In the clinical field, to improve postoperative outcome and establish therapeutic procedures, surgical findings were analyzed to clarify the pathogenesis of cholesteatoma, and the improvement rates in response to each surgical procedure were compared.
In addition, the level of gas exchange through the middle ear membranes and changes in middle ear pneumatic pressure according to gas exchange were examined in patients with otitis media with effusion and eardrum adhesions. In in vitro studies, oxygen inhalation through the normal middle ear mucosa, and gas exchange associated with changes in hemodynamics caused by drugs, particularly changes in partial pressure of oxygen and changes in total pressure in the middle ear are being examined in rabbits.
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