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1998 Fiscal Year Final Research Report Summary

Pathogenesis of adhesive otitis media and study of mechanism of development into the pars tensa cholesteatoma

Research Project

Project/Area Number 09671761
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionTHE JIKEI UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

MORIYAMA Hiroshi  THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY PROFESSOR AND CHAIRMAN, 医学部・耳鼻咽喉科, 教授 (60125036)

Co-Investigator(Kenkyū-buntansha) TSUJI Tomihiko  THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY,ASSISTANT, 医学部・耳鼻咽喉科, 助手 (30236880)
SHIWA Masanori  THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY,ASSISTANT, 医学部・耳鼻咽喉科, 助手 (20235766)
MITANI Yukie  THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY,ASSISTANT, 医学部・耳鼻咽喉科, 助手 (30233894)
KOJIMA Hiromi  THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY,ASSISTANT, 医学部・耳鼻咽喉科, 助手 (60234762)
YAMAGUCHI Tatsuji  THE JIKEI UNIVERSITY SCHOOL OF MEDICINE・OTOLARYNGOLOGY,LECTURER, 医学部・耳鼻咽喉科, 講師 (50182449)
Project Period (FY) 1997 – 1998
KeywordsCHOLESTEATOMA / PKC / KGF / GAS EXCHANGE / ADHESIVE OTITIS MEDIA
Research Abstract

The in situ hybridization technique has been used to determine the mechanism of epithelial proliferation in cholesteatoma, and cultured cell lines, mainly of fibroblasts and keratinocytes, have been analyzed at the protein and gene level. Differentiation and apoptosis of the epithelial cells of cholesteatomas, particularly the effect of protein kinase C on differentiation, and the relationship between the interleukin converting enzyme family (ICE) and apoptosis are being investigated using molecular biological techniques.
The characteristics of terminal differentiation in epithelial cells have been analyzed by comparing the expression of differentiation markers for epithelium cells, especially focusing on protein kinase C (PKC), between cholesteatomatous epithelium, normal epithelium, and the epithelium invaded by psoriasis, a typical inflammatory hyperkeratosis.
The influence of KGF on differentiation markers or cell growth factors will also be analyzed in cultured cell lines to explain the mechanism of normal epithelial differentiation and cell growth inhibitors, and then to assess the possibility of conservative therapy for cholesteatoma.
In the clinical field, to improve postoperative outcome and establish therapeutic procedures, surgical findings were analyzed to clarify the pathogenesis of cholesteatoma, and the improvement rates in response to each surgical procedure were compared.
In addition, the level of gas exchange through the middle ear membranes and changes in middle ear pneumatic pressure according to gas exchange were examined in patients with otitis media with effusion and eardrum adhesions. In in vitro studies, oxygen inhalation through the normal middle ear mucosa, and gas exchange associated with changes in hemodynamics caused by drugs, particularly changes in partial pressure of oxygen and changes in total pressure in the middle ear are being examined in rabbits.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Hiromi Kojima, et al: "Cell proliferation and apoptosis in human middle ear cholesteatoma" Arch Otolaryngol Head Neck Surg.124. 261-264 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiromi Kojima, et al: "Role of Bcl-xL protein in differentiation and apoptosis of human middle ear cholesteatoma epithelium." Arch Otolaryngol Head Neck Surg. 125(in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masanori Shiwa, et al: "Expression of Transforming growth factor-α (TGF-α)in cholesteatoma." J Laryngol Otol. 112. 750-754 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuhiro Tanaka, et al: "A study on epidermal proliferation ability in cholesteatoma." Laryngoscope. 108. 537-542 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 辻 富彦: "鼓膜癒着症の耳管機能 -術前、術後の評価-" 日本耳鼻咽喉科学会会報. 102. 印刷中 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masanori Shiwa, et al: "Role of cytokines in Epidermal proliferation of middle ear cholesteatoma" Cholesteatoma and Mastoid Surgery. 5. 124-127 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiromi Kojima, Yasuhiro Tanaka, Toshihide Tanaka, Masanori Shiwa, Yosuke Kamide, Hiroshi Moriyama.: "Cell proliferation and apoptosis in human middle ear cholesteatoma." Arch Otolaryngol Head Neck Surg.124. 261-264 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masanori Shiwa, Hiromi Kojima, Hiroshi Moriyama.: "Expression of Transforming growth factor-alpha (TGF-alpha) in cholesteatoma." J Laryngol Otol. 112. 750-754 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuhiro Tanaka, Masanori Shiwa, Hiromi Kojima, Hidemi Miyazaki, Yosuke Kamide, Hiroshi Moriyama.: "A study on epidermal proliferation ability in cholesteatoma." Laryngoscope. 108. 537-542 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiromi Kojima, Hidemi Miyazaki, Yasuhiro Tanaka, Masanori Shiwa, Tomohiko Koga, Hiroshi Moriyama.: "Role of Bcl-xL protein in differentiation and apoptosis of human middle ear cholesteatoma epithelium." Arch Otolaryngol head Neck Surg. 125(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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