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1999 Fiscal Year Final Research Report Summary

Laryngeal Cancer Cell Death; Mutotion of Androgen Receptor Gene

Research Project

Project/Area Number 09671767
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionSt. Marianna University School of Medicine

Principal Investigator

TAMOTUSU Urushibata  St.Marianna Unive. Schoold of Med, Dept.of ORL, Assistant Professor, 医学部, 講師 (70130987)

Project Period (FY) 1997 – 1999
KeywordsLaryngeal / Laryngeal Cancer / Cell death / Androgen / Receptor / CAG tandem Repeat / Mutation
Research Abstract

Based on human phonetic differentiation of both sexed and the etiologic finding of laryngeal cancer, androgen would be regarded as significant factor on the development of laryngeal tissue and serious etiologic factor of male-predominant laryngeal cancer. In this study, a laryngeal carcinoma cell line (Hip-2) was examined on the expression of AR protein and mRNA, and responsiveness to androgen, related with the cell proliferation. Immunohistochemical study and electrophoresis of RT-PCR product showed Hep-2 cell expressing AR protein and mRNA, using primers which produce nucleotide sequence of AR between 70 through 367 from the start codon, respectively. The band of gel, however, showed Hep-2 expressing a bit short sized AR mRNA, compared with control. Nucleotide sequential analysis showed that the AR is existence of gene mutation, deleting 9 CAG tandem repeats in the coding modulator region. Above 1 μM, testosterone propionate (TP) induced cell death up to 99%, 3 days after the administration. Immunohistochemical study suggested that Hep-2 cells incorporated TP into their nuclei only through the specific AR and came to cell death, accompanied by enhanced expression of P53, bcl-2 and bax proteins. These data implied that the expression of mutated AR deleting 9 glutamines in the modulator region, combined with androgenic steroids, could trigger the cascade of cell death in Hep-2 cell line.
Supported in part by GASRc9671767.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 漆原保: "喉頭と男性ホルモン-男性ホルモンに対する喉頭組織内標的部位そして癌細胞感受性と細胞死誘導"ホルモンと臨床. 47. 150-154 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamotsu Urushibata: "CAG Tandem Repeat Mutation of Androgen Receptor (AR) Gene ***********"Cold Spring Harbor Laboratory, the 1998 Meeting on Cancer Genetics & Tumor Suppressor Genes. 270 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamotsu Urushibata: "Laryngeal Androgen"Herumon to Rinsho. 47. 150-158 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamotsu Urushibata: "CAG Tandem Repeat Mutation of Androgen Receptor Gene in Laryngeal Carcinoma Cell Line"Cold Spring Harbor, Gancer Genetics of Laboratory, Tumor Suppoessor Gene the 1998 meating on. 270. (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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