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1999 Fiscal Year Final Research Report Summary

The suppression of allograft rejection in retinal pigment epithelial transplantation with ACAID.

Research Project

Project/Area Number 09671799
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionOsaka University

Principal Investigator

TANO Tasuo  Medical School, Osaka University Professor, 医学系研究科, 教授 (80093433)

Co-Investigator(Kenkyū-buntansha) INOVE Toshitsugv  Medical School, Osaka University Associace Professor, 医学系研究科, 助教授 (10213183)
Project Period (FY) 1997 – 1999
KeywordsACAID / allograft rejection / retinal pigment epithelium / TGF-beta
Research Abstract

A variety of antigens inoculated into the anterior chamber (AC) of the eye can elicit systemic suppression of T cell-mediated delayed-type hypersensitivity (DTH). This phenomenon is called anterior chamber-associated immune deviation (ACAID). In this research project, we first examined whether corneal allograft rejection can be suppressed by the induction of ACAID. Splenocytes collected from B10.D2 mouse were injected into the AC of BALB/c mouse. Seven days later, BALB/c mice were immunized subcutaneously by B10.D2 splenocytes. Seven days later, DTH was evaluated by ear swelling response. The eyes of BALB/c mice were grafted with B10.D2 corneal lenticles and alloepithelial rejection was evaluated using the scoring system. The results showed that AC inoculation of splenocytes induced antigen-specific of DHT response as well as supression of epithelial rejection.
In contrast to AC inoculusion of splenocytes, AC inoculusion of retinal pigment epithelium (RPE) collected from Lewis rat nor B10.D2 mouse did not induce ACAID. Our results suggested that RPE could not be antigen presenting cell in retina.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Miyazaki D: "T-cell mediated immune responses in alloepithelial rejection afetr murine keratoepithelioplasty"Invest Ophthalmol Vis Sci. 40. 2590-2597 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori Y: "Detection of HSV mRNA using reverse transcription-polymerase Chain reaction for diagnosis in murine herpetic keratitis model"Jpn J Ophthalmol. 42. 8-11 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永江康信: "ウイルス性網膜炎での眼検体を用いたpolymerase chain reaction法の診断的意義"日本眼科学会雑誌. 102. 509-514 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyazaki D: "Neutrophil chemotaxis induced by corneal epithelial cells after herpes simplex virus type 1 infection"Curr. Eye Res.. 17. 687-693 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yao Y-F: "The antigen-bearing eye and the spleen are indispensable in maintaining anterior chamber-associated immune deviation"Invest Ophthalmol Vis Sci. 38. 534-539 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yao Y-F: "Correlation of anterior chamber-associated immune deviation with suppression of corneal epithelial rejection in mice"Invest Ophthalmol Vis Sci. 38. 292-300 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyazaki D: "T-cell mediated immune responses in alloepithelial rejection after murine keratoepithelioplasty."Invest Ophthalmol Vis Sci. 40. 2590-2597 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori Y: "Detection of HSV mRNA using reverse transcription-polymerase chain reaction for diagnosis in murine herpetic keratitis model."Jpn J Ophthalmol. 42. 8-11 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagae Y: "The Diagnostic Significance of Polymerase Chain Reaction for Ocular Samples in Viral Retinitis"Journal of Jpn Ophthalmolo*cal Society. 102. 509-514 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyazaki D: "Neutrophil chemotaxis induced by corneal epithelial cells after herpes simplex virus type 1 infection."Curr. Eye Res.. 17. 687-693 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yao Y-F: "The antigen-bearing eye and the spleen are indispensable in maintaining anterior chamber-associated immune deviation."Invest Ophthalmol Vis Sci.. 38. 534-539 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yao Y-F: "Correlation of anterior chamber-associated immune deviation with suppression of corneal epithelial rejection in mice."Invest. Ophthal. Vis. Sci. 38. 292-300 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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