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1999 Fiscal Year Final Research Report Summary

Molecular Biologic Study on Etiological Mechanisms and Therapy of Glaucoma

Research Project

Project/Area Number 09671813
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionUniversity of Occupational and Environmental Health (1999)
Wakayama Medical University (1997-1998)

Principal Investigator

TAWARA Akihiko  University of Occupational and Environmental Health, Department of Ophthalmology, Professor, 医学部, 教授 (90117169)

Co-Investigator(Kenkyū-buntansha) KUBOTA Toshiaki  Kyushu University, Department of Ophthalmology, Assistant Professor, 医学部, 講師 (30205140)
Project Period (FY) 1997 – 1999
KeywordsMYOC / TIGR protein / MYOC / TIGR gene / extracellular matrix / immunohistochemistry / goniodysgenetic glaucoma / neovascular glaucoma / iris nevus syndrome / vascular endothelial growth factor
Research Abstract

1) We immunohistochemically examined the localization of MYOC/TIGR protein in the glaucomatous and normal trabecular meshworks. In light microscopic immunohistochemistry, the trabecular meshworks from all the specimens stained positively with anti-MYOC/TIGR polyclonal antibody. Staining was observed in the extracellular matrix. In electron microscopic immunohistochemistry, staining was seen not only in the cytoplasm of the trabecular cells but also in the extracellular matrix. In the extracellular matrix, staining was associated with the long-spacing collagens and fine granular materials. It is concluded that MYOC/TIGR protein is distributed not only in the trabecular cells but also in the extracellular matrix associating with the long-spacing collagens and fine granular materials.
2) The trabecular tissues from enucleated human eyes with exfoliation syndrome were examined histologically and immunohistochemically. Exfoliation fibers were revealed to contain proteoglycans as well as some … More other macromolecules. It was also detected that the iris vessels had some abnormal changes in the syndrome.
3) We examined morphologically the angular region of eyes with inherited glaucoma in rabbits. In the angular region of glaucomatous eyes, a thick abnormal tissue with round-formed cells embedded in the extracellular matrix was located just beneath the plexus. A large amount of extracellular matrix of basal lamina-like material was observed in the thick tissue. In normal eyes, the angular region consisted of well-developed trabecular sheets. These findings support the hypothesis that remaining of a thick subcanalicular tissue because of maldevelopment of the iridocorneal angle is one of the main causes of this type of glaucoma.
4) We examined the trabecular meshwork from human eyes with neovascular glaucoma, as well as from rabbit eyes with experimental neovascularization in the anterior segment of the eye histologically and immunohistochemically. The results suggested growth factor (VEGF) plays an important role in development of anterior segment of the eye, and that invasion of the newly intertrabecular spaces has close relation to glaucoma manifestation.
5) We studied the localization of extracellular matrix in the normal immunohistochemically. The results indicate that laminin and type VI collagen are located diffusely in the trabecular meshwork.
6) We examined the trabecular tissue obtained by trabeculectomy from the patient's with iris nevus syndrome in the left eye to show that disruption of the blood-aqueous barrier may occur in iris-nevus syndrome.
7) We examined the effects of brovincamine fumarate and timolol maleate on choroidal blood flow using laser Doppler flowmetry. The results indicated that intravenous administration of the drugs might not increase the choroidal blood flow. Less

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Ueno, A., Tawara, A., Kubota, T., Ohnishi, Y., et a1.: "Histopathological changes in iridocorneal angle of inherted glaucoma in rabbits"Graefe's Arch Clin Exp Ophthalmol. 237(8). 654-660 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 上村穂高,張野正誉,田原昭彦: "両眼超音波白内障手術後に原因不明の持続性眼圧上昇を来した1例"あたらしい眼科. 16(10). 1409-1412 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 猪俣 孟,田原 昭彦: "眼の組織・病理アトラス157:前部線維柱帯と後部線維柱帯"臨床眼科. 53(12). 1848-1849 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田原昭彦,久保田敏昭,坂本泰二,他: "血管新生緑内障の発症メカニズム-血管新生緑内障の発生病理-"眼科手術. 12(1). 117-120 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田原昭彦: "血管新生緑内障の発生病理"福岡県眼科医会報. 169. 3-6 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田原昭彦,中村多賀雄,吉田綾子,久保田敏昭,他: "虹彩母斑症侯群(iris-nevus syndrome)における虹彩血管の異常"日眼会誌103:259-267. 103(3). 259-267 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tawara, A., Kobata, H., Fujisawa, K., et al.: "Mechanism of intraocular pressure elevation during hemodialysis"Curr. Eye Res.. 17(4). 339-347 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田原昭彦: "血液透析と眼圧"J. UOEH (産業医科大学雑誌). 22(1). 33-43 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田原昭彦,猪俣 孟: "最近の緑内障の薬物治療"株式会杜ミクス. 8 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田原昭彦: "新図説臨床眼科講座"メジカルビュー社. 3 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tawara A., Kobata H., Fujisawa K., Abe T, Ohnishi Y: "Mechanism of intraocular pressure elevation during hemodialysis."Curr Eve Res. 17-4. 339-347 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tawara A., Nakamura T., Yoshida A., et al.: "iris vessel involvement in iris-nevus syndrome"J Jpn Ophthalmol Soc. 103-3. 259-267 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tawara A., Kubota T., Sakamoto T., et al.: "Developmental process of neovascular glaucoma based on histopathological findings"Jpn J Ophthal Sur. 12-1. 117-120 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uemura H., Harino M., Tawara, A.: "Extreme Intraocular Pressure Elevation after Cataract Surgery"Journal of the Eve. 16-10. 721-723 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tawara, A.: "Histopathological study on neovascular glaucoma"J Fukuoka Pref Ophthal Soc. 169. 3-6 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inomata H., Tawara A.: "Anterior and posterior trabecular meshwork"Jpn J Clinic Ophthalmol. 53-12. 1848-1849 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueno A., Tawara A., Kubota T., Ohnishi Y., et al.: "Histopathological changes in iridocorneal angle of inherited glaucoma in rabbits"Graefe's Arch Clin Exp Ophthalmol.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tawara A.: "Intraocular pressure during hemodialysis"J UOEH. 22-1. 33-43 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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