1998 Fiscal Year Final Research Report Summary
Control of fibroblast growth after glaucoma filtration surgery by neutralizing monoclonal antibody against growth factors.
| Project/Area Number |
09671822
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
NISHIKAWA Katsuzo Kanazawa Medical University, Medical School, Professor, 医学部, 教授 (10029960)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Masamichi Kanazawa Medical University, Medical School, Lecturer, 医学部, 助手 (70208966)
TAKAHASHI Nobuo Kanazawa Medical University, Medical Research Institute, Professor, 総合医学研究所, 教授 (20006787)
TAKEUCHI Fumito Kanazawa Medical University, Medical School, Assistant Professor, 医学部, 講師 (70262623)
YOSHITAKE Yoshino Kanazawa Medical University, Medical School, Assistant Professor, 医学部, 講師 (00150764)
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| Project Period (FY) |
1997 – 1998
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| Keywords | glaucoma / growth factor / fibroblasts / monoclonal antibody / growth inhibition / fibroblast growth factor |
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| Research Abstract |
The formation of fibrocellular scar decides failure of glaucoma filtration surgery. Tenon's capsule fibroblasts are the main components of the scar. The experiments were made to obtain greater understanding the pathophysilogy of the scar formation by studying the roles of growth factors such as basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) in the growth of human Tenon's fibroblasts.
Growth of fibroblast cell line established from primary culture of human Tenon's capsule was stimulated by bFGF or EGF in the presence of various concentrations of fetal bovine serum (FBS) in dose-dependent fashion. Strong immuno-fluorescence staining of bFGF was observed in nuclei of the cells, but EGF was not detected. bFGF content in extract of the cells was determined to be 270 ng/lO7cells by sandwich radioimmunoassay with 125I-labeled monoclonal antibody against bFGF and heparin-Sepharose. The expression of bFGF mRNA was also observed in the cultured fibroblasts by RT-PCR method using the specific primers for bFGF.The expression was down-regulated by the addition of bFGF in the medium.
When the fibroblasts were incubated for few days in culture medium supplemented with neutralizing monoclonal antibody against bFGF but without bFGF, viability as measured by the number of colonies formed in the medium without the antibody 1 week later was decreased, indicating that secreted endogenous bFGF acts as a survival factor for these cells. The functional roles of bFGF localized in nuclei of the cultured Tenon's capsule fibroblasts are still unclear.
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