1998 Fiscal Year Final Research Report Summary
The mecanism of metastasis in solid tumors of childhood
| Project/Area Number |
09671833
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
小児外科
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| Research Institution | Kagoshima University |
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Principal Investigator |
TAKAMATSU Hideo Faculty of Medicine Kagoshima University, Professor, 医学部, 教授 (50142427)
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| Co-Investigator(Kenkyū-buntansha) |
OZAWA Masayuki Faculty of Medicine Kagoshima University, Professor, 医学部, 教授 (90136854)
TAHARA Hiroyuki University Hospital Kagoshima University, Research Associate, 医学部・附属病院, 助手 (70236719)
NOGUCHI Hiroyuki Faculty of Medicine Kagoshima University, Assistant Professor, 医学部, 講師 (80198580)
FUKUSHIGE Takahiko University Hospital Kagoshima University, Assistant Professor, 医学部・附属病院, 講師 (70218907)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Neuroblastoma / PNET / Cadherin / catenin |
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| Research Abstract |
Both neuroblastoma and primitive neuroectodermal tumor (PNET) originates in neural crest cells. Cadherins are Ca^<2+> dependent cell-cell adhesion molecules, which plays a crucial role in the cell-cell interaction of cancer cells in tumorigenesis, invasiveness and metastasis. They are divided into several groups, such as E-, P-, N- and others. In this study we investigated the expression patterns of cadherin-catenin complexes in human cell lines of neuroblastomas (five cell lines) and human PNET (one cell line) and eleven tumors surgically resected using a combination of molecular biological and biochemical methods.
N-cadherin was expressed in three neuroblastoma cell lines (SK-N-SH, NB39, 1MR32) but did not in two neuroblastoma cell lines (SK-N-MC, NB1) and one PNET cell line (Muraoka). P-cadherin was expressed only in Muraoka cell line. Cadherin-6 was expressed in five cell lines except NB1 cell line. Cadherin-11 was expressed in three cell lines (SK-N-SH, SK-N-MC, Muraoka). In the cell aggregation assay, N- or P-cadherin positive cell lines aggregated in the presence of Ca^<2+>, but both N- and P- cadherin negative cell lines did not aggregate in the same condition.
All tumors surgically resected expressed both N-cadherin and cadherin-1 1 but did not always expressed cadherin-6 or cadherin-8. These results were not correlated with clinical factors such as age, amplification of N-myc oncogene, clinical stage and pathological findings.
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