1997 Fiscal Year Annual Research Report

口腔の感覚と運動発現に関与する脳幹一酸化窒素合成ニューロンの機能形態学的研究

Research Project

Project/Area Number	09671852
Research Category	Grant-in-Aid for Scientific Research (C)
Research Institution	Osaka University
Principal Investigator	竹村元秀大阪大学, 歯学部, 講師 (70192169)
Co-Investigator(Kenkyū-buntansha)	永瀬佳孝大阪大学, 歯学部, 助手 (50252698) 米原典史大阪大学, 歯学部, 講師 (70124534)
Keywords	一酸化窒素 / NADPH-diaphorase / 三叉神経核 / 感覚 / 運動 / 神経再生 / 疼痛 / 孤束核
Research Abstract	脳幹内の一酸化窒素合成酵素(NOS)発現ニューロンは三叉神経吻側亜核の背内側部(Vodm)、吻外側孤束核(Sn)、傍三叉神経核(para V)及び尾側亜核(Vc)の表層部に多く認められた。HRPの越神経節輸送を利用した下顎神経の種々の末梢枝の中枢投射部位とNOS発現ニューロンを二重標識し、NOS発現ニューロンは舌の体性感覚を伝える舌神経の投射領域に多く分布することを示した。口腔顔面領域を支配する末梢神経の切断や口腔顔面領域にcomplete freunds' adjuvant(CFA)を注入して炎症性刺激を加えた後のNOS発現ニューロンの変化を解析した。VcのNOS発現ニューロンは炎症性刺激に活性が両側性に上昇しやすく、Vodm,Snでは舌神経切断に対して、活性が下降した。CFA注入後の覚痛覚過敏に対してNOSの基質(L-arginine)や阻害剤(nitro-L-arginine methyl ester;L-NAME)投与の効果を確かめた。CFA注射直後の疼痛にはL-NAMEは効果なく、3日目から表れる痛覚過敏症に対して鎮痛作用があった。その作用にL-arginineは拮抗した。下歯槽神経を下顎孔付近で一側性に切断後、断端を元に戻し再生出来る状態にする。一定日数経過後両側臼歯歯髄にHRPを注入し逆行性に標識された神経節ニューロンの数を測定し歯髄に再生したと考えられるニューロンの割合を反対側の歯髄支配ニューロンの数と比較して同定した。この歯髄へ再生するニューロンの割合に対して、L-NAME投与は抑制作用があった。この神経再生賦活効果に対する影響がNOの血管拡張因子としての効果が現れたものか、それとも直接ニューロンの再生を賦活したのかをを血管拡張剤併用によりその効果への影響を調査し、ニューロンへの直接作用であることを確かめた。

Research Products
(18 results)

All Other

All Publications (18 results)

[Publications] Takemura M.: "NADPH-daphorase in the developing rat : Lower brainstem and cervical spinal cord with special reference to the trigemino-solitary complex." Journal of Comparative Neurology. 365. 511-525 (1996)
[Publications] Wakisaka S.: "Neuropeptide Y-immunoreactive primary afferents in the dental pulp and periodontal ligament following nerve injury to the inferior alveolar nerve in the rat." Brain Research. 712. 11-18 (1996)
[Publications] Donovan D.M.: "Transgenic mice : Preproenkephalin and the mu opiate receptor." Annals New York Academy of Science. 780. 19-28 (1996)
[Publications] Bae Y.C.: "Electron microscopic observations of synaptic connections of jaw-muscle spindle and periodontal afferent terminals in the trigeminal motor and supratrigeminal muclei in the cat." Journal of Comparative Neurology. 374. 421-435 (1996)
[Publications] 竹村元秀: "三叉神経核と一酸化窒素:2.特集・神経系の一酸化窒素(監修:俣野)" ブレインサイエンス. 8・2. 39-47 (1997)
[Publications] Ishikawa N.: "Endothelins promote the activation of astrocytes in rat neostriatum through ET_B receptors." European Journal of Neuroscience. 9. 895-901 (1997)
[Publications] Yonehara N.: "Involevement of nitric oxide in re-innervation of rat molar pulp following transection of the inferior alveolar nerve." Brain Research. 757. 31-36 (1997)
[Publications] Yoshida A.: "Organization of the descending projection from the parabrachial nucleus to the trigeminal sensory nuclear complex and spinal dorsal horn in the rat." Journal of Comparative Neurology. 383. 94-111 (1997)
[Publications] Nagase Y.: "Serotonergic axonal contacts on identified cat trigeminal motoneurons and their correlation with medullary rephe nucleus stimulation." Journal of Comparative Neurology. 384. 443-455 (1997)
[Publications] Bae Y.C.: "Identification of gamma aminobutyric acid immunoreactive axon endings associated with mesencephalic periodontal afferent terminals and morphometry of the two types of terminals in the cat supratrigeminal nucleus." Journal of Comparative Neurology. 388. 421-435 (1997)
[Publications] Nakagawa S.: "Ultrastructural observations of synaptic connections of vibrissa afferent terminals in cat principal sensory nucleus and morphometry of related syaptic elements." Journal of Comparative Neurology. 389. 12-33 (1997)
[Publications] Kishimoto H.: "The differences in central projection and spatial distribution of synaptic contacts on motoneurons between two physiologically defined groups of cat jaw-muscle spindle afferents." Journal of Comparative Neurology. 391. 50-63 (1998)
[Publications] Yonehara N.: "Nitric oxide in the rat spinal cord in freund's adjuvant-induced hyperalgesia." Japanese Journal of Pharmacology. 75. 327-335 (1998)
[Publications] Iwase K.: "Ontogeny of NADPH-diaphorase in rat forebrain and midbrain." Anatomy and Embryology. (in press). (1998)
[Publications] Takemura M.: "Central terminals of orofacial primary afferents and NADPH-diaphorase activity in the trigemino-solitary complex of rats." Brain Research. (in press). (1998)
[Publications] Yonehara N.: "Nitric oxide in the rat spinal cord in freund's adjuvant-induced hyperalgesia." Japanese Journal of Pharmacology. 75. 327-335 (1998)
[Publications] Iwase K.: "Ontogeny of NADPH-diaphorase in rat forebrain and midbrain." Anatomy and Embryology. (in press). (1998)
[Publications] Takemura M.: "Central terminals of orofacial primary afferents and NADPH-diaphorase activity in the trigemino-solitary complex of rats." Brain Research. (in press). (1998)

1997 Fiscal Year Annual Research Report

口腔の感覚と運動発現に関与する脳幹一酸化窒素合成ニューロンの機能形態学的研究

Principal Investigator

竹村 元秀 大阪大学, 歯学部, 講師 (70192169)

Research Products

[Publications] Takemura M.: "NADPH-daphorase in the developing rat : Lower brainstem and cervical spinal cord with special reference to the trigemino-solitary complex." Journal of Comparative Neurology. 365. 511-525 (1996)

[Publications] Wakisaka S.: "Neuropeptide Y-immunoreactive primary afferents in the dental pulp and periodontal ligament following nerve injury to the inferior alveolar nerve in the rat." Brain Research. 712. 11-18 (1996)

[Publications] Donovan D.M.: "Transgenic mice : Preproenkephalin and the mu opiate receptor." Annals New York Academy of Science. 780. 19-28 (1996)

[Publications] Bae Y.C.: "Electron microscopic observations of synaptic connections of jaw-muscle spindle and periodontal afferent terminals in the trigeminal motor and supratrigeminal muclei in the cat." Journal of Comparative Neurology. 374. 421-435 (1996)

[Publications] 竹村元秀: "三叉神経核と一酸化窒素:2.特集・神経系の一酸化窒素(監修:俣野)" ブレインサイエンス. 8・2. 39-47 (1997)

[Publications] Ishikawa N.: "Endothelins promote the activation of astrocytes in rat neostriatum through ET_B receptors." European Journal of Neuroscience. 9. 895-901 (1997)

[Publications] Yonehara N.: "Involevement of nitric oxide in re-innervation of rat molar pulp following transection of the inferior alveolar nerve." Brain Research. 757. 31-36 (1997)

[Publications] Yoshida A.: "Organization of the descending projection from the parabrachial nucleus to the trigeminal sensory nuclear complex and spinal dorsal horn in the rat." Journal of Comparative Neurology. 383. 94-111 (1997)

[Publications] Nagase Y.: "Serotonergic axonal contacts on identified cat trigeminal motoneurons and their correlation with medullary rephe nucleus stimulation." Journal of Comparative Neurology. 384. 443-455 (1997)

[Publications] Bae Y.C.: "Identification of gamma aminobutyric acid immunoreactive axon endings associated with mesencephalic periodontal afferent terminals and morphometry of the two types of terminals in the cat supratrigeminal nucleus." Journal of Comparative Neurology. 388. 421-435 (1997)

[Publications] Nakagawa S.: "Ultrastructural observations of synaptic connections of vibrissa afferent terminals in cat principal sensory nucleus and morphometry of related syaptic elements." Journal of Comparative Neurology. 389. 12-33 (1997)

[Publications] Kishimoto H.: "The differences in central projection and spatial distribution of synaptic contacts on motoneurons between two physiologically defined groups of cat jaw-muscle spindle afferents." Journal of Comparative Neurology. 391. 50-63 (1998)

[Publications] Yonehara N.: "Nitric oxide in the rat spinal cord in freund's adjuvant-induced hyperalgesia." Japanese Journal of Pharmacology. 75. 327-335 (1998)

[Publications] Iwase K.: "Ontogeny of NADPH-diaphorase in rat forebrain and midbrain." Anatomy and Embryology. (in press). (1998)

[Publications] Takemura M.: "Central terminals of orofacial primary afferents and NADPH-diaphorase activity in the trigemino-solitary complex of rats." Brain Research. (in press). (1998)

[Publications] Yonehara N.: "Nitric oxide in the rat spinal cord in freund's adjuvant-induced hyperalgesia." Japanese Journal of Pharmacology. 75. 327-335 (1998)

[Publications] Iwase K.: "Ontogeny of NADPH-diaphorase in rat forebrain and midbrain." Anatomy and Embryology. (in press). (1998)

[Publications] Takemura M.: "Central terminals of orofacial primary afferents and NADPH-diaphorase activity in the trigemino-solitary complex of rats." Brain Research. (in press). (1998)

竹村元秀大阪大学, 歯学部, 講師 (70192169)