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1998 Fiscal Year Final Research Report Summary

Molecular analysis of pathogen in periodontopathic bacteria

Research Project

Project/Area Number 09671871
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

ISHIHARA Kazuyuki  Tokyo Dental College, Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (00212910)

Co-Investigator(Kenkyū-buntansha) KIMIZUKA Ryuta  Tokyo Dental College, Faculty of Dentistry, Assistant, 歯学部, 助手 (90287178)
YAMANAKA Ayumi  Tokyo Dental College, Faculty of Dentistry, Assistant, 歯学部, 助手 (40231667)
MIURA Tadashi  Tokyo Dental College, Faculty of Dentistry, Assistant, 歯学部, 助手 (10266570)
Project Period (FY) 1997 – 1998
KeywordsProtease / Hemolytic activity / Treponema denticola / Fimbriae / Expression regulation / Actinobacillus actinomycetemcomitans / Pathogenicity / Bacteroides forsythus
Research Abstract

Adult periodontitis was Infectious disease and it induce tooth loss. It cause a mastication problem and finally induce poor quality of life in patients with periodontal disease. As many attempts to clarify pathogen of the disease have been performed, the molecular mechanisms by which these pathogens exert their effects are poorly understood. To clarify the pathogenic mechanism of potential periodontopathic bacteria, we tried to characterize genes encoding pathogens from Actionbacillus actinomycetemcomitans, Bacteriodesforsydius and Treponema denticola.
We determined the fimbriae gene (fap) of A.actinomycetemcomitans to characterize its role in colonization. The fimbriated strain of this microorganism lost fimbriae upon passaging onto solid medium. W found that expression of the fap gene was decreased in non-fimbriated strain (Microbiol. Pathog. 1997, 23 : 63-69).
Proteases were known to exert a cytotoxic effects to host tissue. We detennined the sequence of a protease gene (prH) of B.for … More sythus which is difficult to get enough growth in synthetic medium for a characterization. A molecular mass of the protease is 47,873 and the PrtH possessed hemolytic activity (Imfect. Immun. 1997, 65 : 4888-4891).
Several reports indicated the systemic chemotherapy failure was caused by colonization of Helicobcterpylori in oral cavity. We evaluated the antagonistic effects between oral microorganism and H.pylon. H.pylon was coaggregated with P.gingivalis and F.nucreatum. On the other hand, several oral bacteria inhibits growth of H.pylon (FEMS Microbial. Lett. 1997, 152 : 355-361).
Evaluation of a pathogenicity using wild type strain and single gene deficient mutant is the most effective way to evaluate its pathogenicity. We constructed a knockout mutant of the prolyl-phenylalanine specific protease (dentilisin) in T denticola and characterized the mutant. Surface hydrophobicity of this microorganism decreased dramatically and ability of abscess formation decreased in the mutant. In addition, we found that dentilisin is associated with organization of outer sheath protein. These results indicated that dentilisin exert a cytotoxic effects to host tissue directly (J.Bacteriol. 1998, 180 : 3837-38449). Less

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Kazuyuki Ishihara: "Cloning and sequence analysis of the fimbriae associated protein (fap) gene from Actinobacillus actinomycetemcomitans" Microb. Pathog.23・2. 63-69 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuyuki Ishihara: "Dentilisin activity affects the organization of the outer sheath of Treponema denticola" J.Bacteriol.180・15. 3837-3844 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuyuki Ishihara: "Oral bacteria inhibit the Helicobacter pylori growth" FFMS Microbiol. Lett.152・2. 355-361 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toru Saito: "Cloning, expression, and sequencing of a protease gene from Bacteroides forsythus ATCC 43037 in Escherichia coli" Infect. Immun.65・11. 4888-4891 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toru Saito: "Adherence of oral streptococci to an immobilized antimicrobial agent" Arch. Oral. Biol.42・8. 539-545 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junichi Takahashi: "Dynamics of serum immunoglobulin G avidity for Porphyromonas gingivalis in adult periodontitis" J. Periodontol.69・3. 367-373 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honma, K.: "Genetic control of immune reoponses to a synthetic fimbrial antigen of Actinobacillus actinomycetemcomitans" Microbiol. Immunol.41・8. 609-614 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okuda, K.: "Role for the S-layer of Campylobacter rectus ATCC 33238 in complement mediated killing and phagocytic killing by leukocyte from guinea pig and human peripheral blood" Oral Dis.3. 113-120 (1977)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihara, K.Honma, K., Miura, T., Kato, T.and Okuda, K.: "Cloning and sequence analysis of the fimbriae associated protein (fap) gene from Actinobacillus actinomycetemcomitans." Microb.Pathog.23. 63-69 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuda, K., Kigure, T., Yamada, S., Kaneko, T., Ishihara, K., Miura, T., Kato, T.and Takazoe, I.: "Role for the S-layer of Compylobacter rectus ATCC 33238 in complement mediated killing and phagocytic killing by leukocyte from guinea pig and human peripheral blood." Oral Dis.3. 113-120 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, T., Takatsuka, T., Kato, T., Ishihara, K.and Okuda, K.: "Adherence of oral Streptococci to an immobilized antimicrobial agent." Archs oral Biol.42. 539-545 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, J., Saito, A., Nakagawa, T., Yamanda, S., Ishihara, K.and Okuda, K.: "Dynamics of serum immunoglobulin G avidity for Porphyromonas gingivalis in adult periodontitis." J.Periodontol.69. 367-373 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, T., Ishihara, K., Kato, T.and Okuda, K.: "Cloning, expression, and sequencing of a protease gene from Bacteroides forsythus ATCC 43037 in Escherichia coli." Infect.Immun.65. 4888-4891 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honma, K., Ishii, N., Kato, T., Ishihara, K., and Okuda, K.: "Genetic control of immune responses to a synthetic fimbrial antigen of Actinobacillus actinomycetemcomitans." Microbiol.Immunol.41. 609-614 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara, K.Miura, T., Kimizuka, R.and Okuda, K.: "Oral bacteria inhibit the Helicobacter pylori growth." FEMS Microbial.Lett.152. 355-361 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara, K., Kuramitsu, H.K., Miura, T.and Okuda, K.: "Dentilisin activity affects the organization of the outer sheath of Treponema denticola." J.Bacteriol.180. 3837-3844 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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