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1999 Fiscal Year Final Research Report Summary

Phytopreventive and Phytotherapeutic Application to Dentistry - Anti-dental caries and anti-periodontal disease activity, and oral retentivity after application of edible phytochemical flavonoids and alkaloids -

Research Project

Project/Area Number 09671911
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionAsahi University

Principal Investigator

TSUCHIYA Hironori  Asahi University, School of Dentistry, Assoc.Prof., 歯学部, 助教授 (30131113)

Co-Investigator(Kenkyū-buntansha) FUJIWARA Shuu  Asahi University, School of Dentistry, Assoc.Prof., 歯学部, 助教授 (50229069)
SATO Masaru  Asahi University, School of Dentistry, Assoc.Prof., 歯学部, 助教授 (70154101)
Project Period (FY) 1997 – 1999
KeywordsPhytoprevention and phytotherapy / Anti-dental caries / Anti-periodontal disease / Flavonoids / Alkaloids / Antibacterial activity / Oral retentivity / Pharmacological mechanism
Research Abstract

To apply the phytopreventive and phytotherapeutic strategies to dentistry, the flavonoidal and alkaloidal components in edible plants were studied concerning the anti-dental caries and anti-periodontal disease activity, the retentive property in oral cavity, and the pharmacological mechanism.
1. Flavonoids : (1) Screenings of various plants provided an antibacterial flavone with the potent utility for dental caries and MRSA infection. (2) An HPLC method was developed for the simultaneous determination of eight catechins in saliva. (3) The method was applied to quantitative studies of pharmacologically active polyhydroxyflavonoids. (4) Oral pharmacokinetic studies revealed that anti-caries catechins were retained in oral cavity after mouthrinsing, and such retentivity was due to their adsorption to different oral sites. (5) The caries-preventive utility of catechins was evaluated comparatively with chlorhexidine as a reference agent. (6) Membrane fluidity-reducing effects were found to u … More nderlie the anti-caries and anti-bacterial actions of flavonoids. (7) In screening of edible plants, onion was found to contain a flavonoidal collagenase-inhibitor , followed by studies to develop the phytomedicine against periodontal diseases.
2. Alkaloids : (1) Beta-carbolines inhibited the growth of oral bacteria responsible for dental caries and periodontal diseases. (2). An HPLC method was developed for the determination of pharmacologically active β-carbolines in edible and medicinal plants. (3) In screenings of functional foodstuffs, two β-carbolines in soy sauce inhibited human platelet aggregation, followed by educidation of their anti-platelet mechanism. (4) A series of phytochemical studies suggested the interaction between alcoholism-relating β-carboline and general/local anesthetics, and the responsibility of metabolic deficiency of dietary β-carboline for the pathogenesis of a certain mental disorder. Beta-carboline alkaloids have been studied as a possible etiological factor of anesthetic tolerance by chronic alcohol intake and infantile autism. Less

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Hironori Tsuchiya, Masaru Sato, Hirotsugu Kato et al: "Simultaneous determination of catechins in human saliva by high-performance liquid chromatography"Journal of Chromatography B. 703. 253-258 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hironori Tsuchiya, Masaru Sato, Hirotsugu Kato et al: "Nanoscale analysis of pharmacologically active catechines in body fluids by HPLC using borate complex extraction pretreatment"Talanta. 46. 717-726 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masaru Sato, Shuu Fujiwara and Hironori Tsuchiya,: "Phytochemical flavones with the antibacterial activity against methicillin-resistant Staphylococcus aurens"Dentistry in Japan. 34. 133-135 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hironori Tsuchiya: "High-performance liquid chromatographic analysis of polyhydroxyflavones using solid-phase borate-complex extraction"Journal of Chromatography. 720. 225-230 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 土屋博紀,加藤博詞,林英明: "緑茶カテキン類の口腔内保持(1)-洗口後の唾液中カテキン濃度-"歯科薬物療法. 17. 138-146 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 土屋博紀,加藤博詞: "緑茶カテキン類の口腔内保持(2)-ハイドロキシアパタイト,粘膜細胞,タンパクへの吸着-"歯科薬物療法. 17. 147-154 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hironori Tsuchiya, Takashi Miyazaki et al: "High-performance liquid chromatographic analysis of chlorhexidine in saliva after mouthrinsing"Caries Research. 33. 156-163 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hironori Tsuchiya: "Effects of green tea catechins on membrane fluidity"Pharmacology. 59. 34-44 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hironori Tsuchiya, Hideaki Hayashi, Masaru Sato et al: "Quantitative analysis of all types of β-carboline alkaloids in medicinal plants and dried edible plants by high-performance liquid chromatography with selective ……"Phytochemical Analysis. 10. 247-253 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hironori Tsuchiya, Hiroshi Shimizu et al: "Beta-carboline alkaloids in crude drugs"Chemical and Pharmaceutical Bulletin. 47. 440-443 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hironori Tsuchiya, Masaru Sato and Ikuo Watanabe: "Antiplatelet activity of soy sauce as functional seasoning"Journal of Agricultural and Food Chemistry. 47. 4167-4174 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hironori Tsuchiya, Masaru Sato and Hirotsugu Kato: "High-performance liquid chromatographic analysis of (+)-catechin and (-)-epicatechin in human plasma"Analytical Pharmacology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hironori Tsuchiya and Munekazu Iinuma: "Reduction of membrane fluidity by antibacterial sophoraflavanone G isolated from Sophora exigua"Phytomedicine. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hironori Tsuchiya and Tokishi Hayashi: "A possible link between β-carboline metabolism and infantile autism"Medical Hypotheses. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hironori Tsuchiya et al.: "Simultaneous determination of catechins in human saliva by high-performance liquid chromatography"Journal of Chromatography B. 703. 253-258 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya et al.: "Nanoscale analysis of pharmacologically active catechins in body fluids by HPLC using before complex extraction pretreatment"Talanta. 46. 717-726 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaru Sato et al.: "Phytochemical flavones with the antibaterial activity against methicillin-resistant Staphylococcus aureus"Dentistry in Japan. 34. 133-135 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya: "High-performance liquid chromatographic analysis of polyhydroxyflavones using solid-phase borate-complex extraction"Journal of Chromatography B. 720. 225-230 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya et al: "Retention of green tea catechins in oral cavity (1) - Salivary concentrations of catechins after mouthrinsing -"Oral Therapeutics and Pharmacology. 17. 138-146 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya et al: "Retention of green tea catechins in oral cavity (2) - Adsorption to hydroxyapatite, mucosal cells and proteins -"Oral Therapeutics and Pharmacology. 17. 147-154 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya et al.: "High-performance liquid chromatographic analysis of chlorhexidine in saliva after mouthrinsing"Caries Research. 33. 156-163 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya: "Effects of green tea catechins on membrane fluidity"Pharmacology. 59. 34-44 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya et al.: "Quantitative analysis of all types of β-carboline alkaloids in medicinal plants and dried edible plants by high-performance liquid chromatography with selective fluorometric detection"Phytochemical Analysis. 10. 247-253 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya et al.: "Beta-carboline alkaloids in crude drugs"Chemical and Pharmaceutical Bulletin. 47. 440-443 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya et al.: "Antiplatelet activity of soy sauce of functional seasoning"Journal of Agricultural and Food Chemistry. 47. 4167-4174 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya et al.: "High-performance liquid chromatographic analysis of (+)-catechin and (-)-epicatechin in human plasma"Analytical Pharmacology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya et al.: "Reduction of membrane fluidity by antibacterial sophoraflavanone G isolated from Sophora exigua"Phytomedicine. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hironori Tsuchiya et al.: "A possible link between β-carboline metabolism and infantile autism"Medical Hypotheses. in press.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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