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1998 Fiscal Year Final Research Report Summary

Opsonophagocytic effect of antibody against recombinant 40-kDa OMP of P.gingivalis on neutrophils and induction of Th1 immune responses

Research Project

Project/Area Number 09671935
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionNihon University

Principal Investigator

SAITO Shigeno  Nihon University School of Dentistry at Matsudo Biochemistry Instructor, 松戸歯学部, 講師 (60072394)

Project Period (FY) 1997 – 1998
KeywordsNeutrophil / phagocytosis / Porphyromonas gingivalis / opsonization / Th1 immune responses
Research Abstract

Porphyromonas gingivalis is associated with the initiation and progression of adult periodontitis. The outer membrane proteins of the bacteria are potentially important targets for interaction with host defense systems. A 40-kDa OMP is conserved among many strains of P.gingivalis. For the development of recombinant 40-kDa OMP as a vaccine for passive immunization, the elucidation of the roles of the anti-r40-kDa OMP antibody in the host defense against P.gingivalis is essential. We demonstrated that r40-kDa OMP antibody in the presence of human complement successfully opsonized [^3H]-thymidine-labelled P.gingivalis as a target for phagocytosis by HL-60 cells differentiated with DMSO.The phagocytized bacteria were then intracellularly killed and lysed, and the radioactive degradation debris egested into the culture medium. We conclude that an antibody against r40-kDa OMP has an opsonic activity on human neutrophil function for phagocytosis of P.gingivalis. We might speculate that Th2 cells can be protective via the provision of help for antibody synthesis and that Th1 and CD8^+ T lymphocytes might be destructive via the prominent production of IFN-gamma and the potential stimulation of macrophage secretion of IL-1 and subsequent bone-destructive activity. Thus, T-cell features such as help[for antibody are important modulators of periodontal inflammation and therefore may be considered targets for disease intervention.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] Shigeno Saito: "Oponoprrjoutic effect of antibody against reiompinant conserved 40・kDa OMP of Pisingivalis on DMSO-differentiated HL-60" Journal of Periodontology. (印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SAITO Shigeno et al.: "Opsonophagocytic effect of antibody against recombinant conserved 40-kDa OMP of Prophyromonas gingivalis on dimethl sulfoxide differentiated HL-60 cells" Journal of Periodontology. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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