1999 Fiscal Year Final Research Report Summary
Evaluation of periodontal pathogenic microorganisms and periodontitis using rapid DNA probe method.
| Project/Area Number |
09671965
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | TOKYO DENTAL COLLEGE |
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Principal Investigator |
TAKAHASHI Junichi Tokyo Dental College, Dept. of Dentistry, Assistant, 歯学部, 助手 (30287180)
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| Co-Investigator(Kenkyū-buntansha) |
KOMIYA Akiyo Tokyo Dental College, Dept. of Dentistry, Assistant, 歯学部, 助手 (00307381)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Rapid DNA probe / Porphyromonas gingivalis / Actinobacillus actinomycelemcomitans / Periodontal disease-associated bacteria / Rapid diagnosis / Periodontal initial therapy |
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| Research Abstract |
The aim of the present study was to develop a rapid DNA probe method for the diagnosis of periodontitis that can be used in the dental clinics. The method minimizes the use of water bath for ordinary hybrydization and washing in order to shorten the total time. Samples were hybridized with boiled DNA probe, and then washed thoroughly with 0.2×SSC/0.1% SDS at 75℃. Detection process could be completed within 2 hours. Detection level was more than 10ィイD14ィエD1 bacterial cells/sample.
Subgingival plaque samples were taken from 468 sites in 55 patients with periodontitis before the periodontal initial therapy. After the periodontal therapy of scaling and rootplaning, 58 sites in 9 patients with periodontitis were bacteriologically and cliically evaluated. When the DNA probe method was compared with the culture method, the acuracy was 88% for P. gingivalis, 67% for A. actinomycetemcomitans. A significant association was found between the detection of P. gingivalis and PD, BOP (x 2 test, p<0.01). A significant association was also shown between the detection of A. actinomycetemcomitans and PD in patients whose ages were 35 or older (x 2 test, p<0.01). The relationship between A. actinomycetemcomitans and BOP was not significant. The detection rate of A. actinomycetemcomitans was highest in teenagers. At shallow periodontal pocket sites (PD<4mm) in teenagers, no P. gingivalis was found, while 22% of the sites harbored A. actinomycetemcomitans. After the therapy, the proportions of P. gingivalis decreased significantly only in the clinically resolved sites(% 2 test, p<0.01). However, A. actinomycetemcomitans seemed to persist after the therapy.
The rapid DNA probe method appears promising as an efficient tool for rapid diagnosis, selection of treatment modalities, and microbiological evaluation of the treatment outcome.
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