Experimental Periapical Lesion in Non-insulin Dependent Diabetic Rat (GK Rat).

2000 Fiscal Year Final Research Report Summary

• Project/Area Number: 09671973
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Dependent of Endodontics, School of Dentistry, Aichi Gakuin University
• Principal Investigator: NAKAMURA Hiroshi Aichi-Gakuin University, School of Dentistry, Professor, 歯学部, 教授 (40064878)
• Co-Investigator(Kenkyū-buntansha): SHIBATA Naoki Aichi-Gakuin University, School of Dentistry, Assistant, 歯学部, 助手 (60291770)
• Project Period (FY): 2000
• Keywords: GK rat / Periapical Lesion / Non-insulin Dependent Diabetes

Research Abstract

The purpose of the present study was to investigate histologically and histometrically the effect of a sucrose solution on the periapical lesion of spontaneous non-insulin dependent diabetes mellitus rats (GK Rat) which were made by Goto ＆ Kakizaki. Twenty GK rats and 20 JCL Wistar rats (JCL Rat) were used in this study. All animals received a pulpal exposure treatment in the left mandibular first molar. Then, the animals were divided equally into two groups of JCL rats (J-1, J-2) and two groups of GK rats (G-1, G-2). J-1 and G-1 received a normal laboratory diet and water, while J-2 and G-2 received a normal laboratory diet and 30% sucrose solution. The animals of all 4 groups were sacrificed 2 and 4 weeks after pulpal exposure for histological observation. The blood glucose tests showed higher values at 4 and 12 weeks in G-1 and G-2 than in J-1 and J-2. Pulpal tissues (PT) and periapical tissues (PAT) of the mandibular first molar were examined histologically. Moreover, the area of the periapical lesion in PAT was measured histometrically. Histologically, the alveolar bone resorption in PAT was severer in J-2 than that in G-2 throughout the experimental period. The histometrical study revealed that the area of the periapical lesion was significantly larger in G-2 at 2 and 4 weeks than that in J-2 (p<0.01). The results of the present study indicate that the metabolic condition of non-insulin dependent diabetes mellitus may enhance the development of periapical lesions in rats.