1998 Fiscal Year Final Research Report Summary
A study on dentin treatment by collagenase to expose mineral-rich surface layer
| Project/Area Number |
09672002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Iwate Medical University |
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Principal Investigator |
ARAKI Yosima Iwate Medical University, School of Dentistry, Professor (20005036)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Minoru 岩手医科大学, School of Dentistry, Professor (10005100)
SAITO Setsuo 岩手医科大学, School of Dentistry, Research assistant (70137537)
MIURA Hiroko University of Tokyo, Graduate school, Instructor (10183625)
HARAGUCHI Katsuhiro Health Sciences University of Hokkaido, 歯学部, Instructor (80198902)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Adhesion / dentin / acid etching / collagenase etching / Raman spectroscopy |
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| Research Abstract |
Adhesion of restorative resin to dentin cavity is performed by forming the hybrid layer of collagen fiber with bonding resin after applying bonding material to acid-etched dentin surface. However bonding resin essentially bond to tooth mineral more strongly than collagen fiber. Thus, we tried to prepare mineral rich dentin surface by selective etching of organic phases of dentin surface layer with collagenase, pepsidase, and sodium hypochlorite. First, prior to evaluate the effect of the treatment on the dentin surface modification, laser Raman spectroscopy of intact surface of dentin was performed. In the spectrum, Raman scattering peaks from inorganic phosphate groups, protein amide and methylene groups were detected. The methylene peaks were broadened due to strong binding of collagen with apatite mineral. The meneral components were selectively etched by treatment with 30%phosphoric acid for 2 minutes or 20%citric acid for 1 minute, showing the change in peak intensity ratio of methylene to phasphate. But dentin protein did not get denatured with these acid treatments. The organic components in the surface layer of dentin were deduced by the treatments of 10%sodium hypochlorite and the mixed solution of collagenase and pepsidase. High resolution analysis of the dentin surface reviealed intact collagen remaining below the etched surface layer. The combined treatment of collagenaze etching with phosporic acid etching or collagenase etching with hypochlotite etching was more effective to dissolve the organic components. The effect of dentin treatment varied among the portion of the tissue.
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[Journal Article] Biocompatibility and osteoconduction of bone defect filler consisting of alfa-tricalcium phosphate and 50%-deacetylated chitosan based binders.1998
Author(s)
Kon, R., Ichimaru, T., Nagata, K., Saito, S., Katsura, H., Araki, Y.
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Journal Title
Dent J Iwate Med Univ. 23(3)
Pages: 205-215
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of collagenase etching treatment on dentin surface modification1997
Author(s)
Araki, Y., Endo, K., Kawashima, I., Yamane, Y., Ohno, H., Haraguchi, K., Matsuda, K., Miura, H.
Organizer
The 29th Academic Meeting on Dental Materials and Devices
Place of Presentation
Tolcyo
Year and Date
1997-04-05
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Infra-red Raman spectra of primary and permanent tooth enamel and dentin1997
Author(s)
Araki, Y., Endo, K., Kawashima, I., Yamane, Y., Ohno, H., Miura, H., Matsuda, T., Matsuda, K.
Organizer
The 29th academic Meeting on Dental Materials and Devices
Place of Presentation
Tolcyo
Year and Date
1997-04-05
Description
「研究成果報告書概要(欧文)」より
