1998 Fiscal Year Final Research Report Summary
EFFECTS OF RADIATION ON OSTEOBLAST-LIKE CELLS AND THE BEST TIME FOR BONE GRAFTING AFTER IRRADIATION
| Project/Area Number |
09672033
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | ASAHIKAWA MEDICAL COLLEGE |
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Principal Investigator |
NISHIMURA Taiichi ASAHIKAWA MEDICAL COLLEGE,DEPARTMENT OF ORAL & MAXILLOFACIAL SURGERY,INSTRUCTOR, 医学部, 講師 (30113749)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEKAWA Masanori ASAHIKAWA MEDICAL COLLEGE,DEPARTMENT OF ORAL & MAXILLOFACIAL SURGERY,ASSISTANT, 医学部, 助手 (50216876)
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| Project Period (FY) |
1997 – 1998
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| Keywords | osteoblast-like cell / osteoblast phenotype / proliferation / alkaline phosphatase activity / differentiation |
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| Research Abstract |
1. Cell proliferation
(1) In nonirradiation group, cells exhibited continued growth throughout the culture period.
(2) In irradiation group, cell proliferation was inhibited by irradiation according to doses. Although a 5 Gy to 10 Gy dose of irradiation inhibited cell proliferation, cells regrew gradually according to time in culture. A 15 Gy dose of irradiation inhibited cell proliferation and cells did not regrow throughout the cultureperiod.
2. Expression of osteoblast phenotype
(1) In the absence of ascorbic acid, both alkaline phosphatase activity and parathyroid hormone mediated cAMP production plateaued at low level. The difference between nonirradiation group and irradiation group was not statistically significant.
(2) In the presence of ascorbic acid, alkaline phosphatase activity in osteoblast-like cells was increased gradually according to time in culture and showed peak expression at about 14 days. Alkaline phosphatase activity was inhibited by irradiation according to doses.
In summary, these results suggest that proliferation and differentiation of osteoblast-like cell was inhibited according to time in culture when osteoblast-like cells were irradiated at proliferating stage. But the best time for bone grafting after irradiation was not detected because effects of irradiation on proliferation and differentiation of osteoblast-like cell may be different at various stages.
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