1998 Fiscal Year Final Research Report Summary
Application of octacalcium phosphate for bone substitutes and a carrier for bone morphogenetic protein
| Project/Area Number |
09672035
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
KAMAKURA Shinji School of Dentistry, Tohoku University, Research Associate, 歯学部, 助手 (80224640)
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| Co-Investigator(Kenkyū-buntansha) |
SASANO Yasuyuki School of Dentistry, Tohoku University, Research Associate, 歯学部, 助手 (30196191)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Bone / Bone Formation / Bone Repair / Octacalcium Phosphate / Calcium Phosphate |
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| Research Abstract |
Synthetic octacalcium phosphate (OCP) enhances bone lbrmation if implanted into the subperiosteal region of murine bone. Such implanted QCP may be resorbed by the multinucleated giant cells which share some ultrastmctural characteristics with osteoclasts and replaced by bone with time. We hypothesized that OCP could be used as an effective bone substitute. To test this hypothesis, the present study was designed to investigate if bone repair in a rat skull defect is enhanced by the implantation of OCP.
Rats were divided into two groups : OCP-treated animals and untreated controls. Six rats from each group were fixed at4, 12, and 24 weeks after implantation. A full-thickness standardized trephine defect was made in the parietal bone and synthetic OCP was implanted into the defect. After being examined radiographically, the specimens were decalcifled and processed for histology.
OCP implantation significantly promoted bone repair compared to the controls. A statistical analysis showed an increase in the area of radiopacity within the skull defect between week 4 and week 12. Histologically, bone was formed on the implanted OCP and along the defeut margin atweek4. At week 12, the implanted OCP was surrounded by newly formed bone. At week 24, the defect was almost completely filled with bone. In the control, bone formation was observed only along the defect margin.
The present results demonstrate that OCP could be used as an effective bone substitute.
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[Publications] Kagayama, M,Sasano, Y,Zhu, J-X,Hirata, M,Mizoguchi, I,Kamakura, S: "Epithelial rests colocalized with cementoblasts forming acellular cementum but not with cementoblasts forming cellular cementum." Acta Anat. 163. 1-9 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kagayama, M,Sasano, Y,Sato, H,Kamakura, S,Motegi, K,Mizoguchi, I: "Confocal microscopy of dentinal tubules in human tooth stained with alizarin red." Anat Embryol. (in press). (1998)
Description
「研究成果報告書概要(欧文)」より
