1998 Fiscal Year Final Research Report Summary
STUDY OF MECHANISMS OF DIFFERENTIATION AND RESISTANCE TO ANTI-CANCER AGENT OF EMBRYONAL CARCINOMA CELLS
| Project/Area Number |
09672046
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | OSAKA CITY UNIVERSITY MEDICAL SCHOOL (1998)
Osaka University (1997) |
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Principal Investigator |
MORITA Takashi OSAKA CITY UNIVERSITY MEDICAL SCHOOL,PROFESSOR, 医学部, 教授 (70150349)
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| Co-Investigator(Kenkyū-buntansha) |
IWAI Soichi OSAKA UNIVERSITY,FACULTY OF DENTISTRY,
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| Project Period (FY) |
1997 – 1998
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| Keywords | embryonal carcinoma cell / cisplatin / apoptosis / cell cycle / RecA / Rad51 / antisense DNA |
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| Research Abstract |
(1) We demonstrated that cisplatin induced growth suppression followed by induction of differentiation in teratocarcinoma F9 cells. In this study, we investigated the mechanism of the effect of cisplatin for cell cycle progression in F9 cells focusing on the change of p34^<cdc2>. By the treatment, the level of the expressionof cdc2 mRNA did not change, but the half life of p34^<cdc2> was greatly reduced, which would result in the arrest the cell cycle at the late S to G2/M.
(2) The mammalian Rad51 gene is a homolog of the yeast Rad5l and E.coli RecA genes, which are related to the repair of DNA double-strand breaks and are also involved in recombination repair and various SOS responses to DNA damage by g-irradiation and alkylating agent like anticancer agent. In this study, we demonstrated that Rad51 antisense oligonucleotides enhances the radiosensityivity of mouse malignant gliomas in vivo and in vitro, suggesting the potentiation for radiation therapy in malignant gliomas by inhibiting DNA double-strand break repair.
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Research Products
(10 results)
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[Publications] Yoshida, K., Kondoh, G., Matsuda, Y., Habu, T., Nishimune, T., Morita, T.: "The mouse RecA-like Gene, Dmcl is required for homologous chromosome synapsis during meiosis." Mol.Cell. 1. 707-718 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Tsuzuki, T.Fujii, Y., Sakumi, K., Tominaga, Y., Nakao, K., Sekiguchi, M., Matsushiro, A., Yoshimura, Y., Morita, T.: "Targeted disruption of the Rad51 gene leads to lethality in embryonic mice." Proc.Natl.Acad.Sci.USA. 93. 6236-6240 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamamoto, A., Taki, T., Yagi, H., Habu, T., Yoshida, K., Yoshimura, Y., Yamamoto, K., Matsushiro, A., Nishimune, Y.and Morita, T.: "Cell cycle-dependent expression of the mouse Rad51 gene in proliferating cells." Mol.Gen.Genet.251. 1-12 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Taki, T., Ohnishi, T., Yamamoto, A, Mori, K., Iwaisako, K., Hiraga, S., Arita, N., Izumoto, S., Hayakawa, T., Morita, T.: "Antisense inhibition of the RAD51 enhances radiosensitivity." Biochem.Biophys.Res.Commun.223. 434-438 (1996)
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「研究成果報告書概要(欧文)」より
