• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2000 Fiscal Year Final Research Report Summary

Studies on Natural Anti-ulcer Agents based on Eradication of Helicobacter pylori

Research Project

Project/Area Number 09672143
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemical pharmacy
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

YOSHIDA Takashi  Okayama University, Fac.Pharm.Sci., Professor, 薬学部, 教授 (20025696)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI Shoko  Okayama University, Fac.Pharm.Sci., Res.Assoc., 薬学部, 助手 (20243488)
ITO Hideyuki  Okayama University, Fac.Pharm.Sci., Res.Assoc., 薬学部, 助手 (70253002)
HATANO Tsutomu  Okayama University, Fac.Pharm.Sci., Assoc.Prof., 薬学部, 助教授 (50127578)
HIRAI Yoshikazu  Jichi Med.University, Medical School, Professor, 医学部, 教授 (00127581)
Project Period (FY) 1997 – 2000
KeywordsHelicobacter pylori / Antioxidant polyphenol / Antibacterial activity / Iridal-type triterpenoid / Tellimagrnadin I / MRSA / Inhibition of histamine-release
Research Abstract

Based on the fact that polyphenolics such as green tea catechins and polyphenols in cocoa are known to show anti-Helicobacter pylori (HP) activity, polyphenolic components of various species of Leguminosae, Geraniaceae, Euphorbiaceae, Betulaceae and Iridaceae were examined in order to discover effective anti-HP agents. This study led to the isolation of 27 new antioxidative polyphenolics, which include flavonoids, hydrolysable tannin monomers and oligomers, and 7 new iridal-type triterpenoids with piscicidal activity. Their structures were elucidated on the basis of spectral and chemical evidence. Among these compounds, hydrolysable tannins exhibited potent anti-HP activity with MIC 3.13〜12.5μg/ml, whereas procyanidins and EGCG had MIC 12.5〜100μg/ml. The most potent anti-HP activity was exhibited by tellimagrandin I, corilagin and strictinin. This activity was bactericidal, but not statistical. These active polyphenols were not sensitive to ordinary pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Batilus subtilis and Pseudomonus aeruginosa, indicating their specific action to HP.Tellimagrandin I and corilagin were also found to have synergestic action with β-lactam on methicillin-resistant Staphylococcus aureus (MRSA). The MIC of oxacillin against MRSA was reduced to 1/100〜1/500 upon addition of these tannins (50μg/ml), suggesting a promising strategy to overcome the MRSA.
In addition, the hydrolyzable tannins showed inhibitory effects on histamine-release from rat mast cells, which was induced by superoxide anion and/or compound 48/80.

  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Hatano,T.,Ito,H.,Yoshida,T. et al.: "Phenolic Constituents of Licorice. VII. A New Chalcone with a Potent Radical Scavenging Activity and Accompanying Phenolics from Liquorice."Chem.Pharm.Bull.. 45. 1485-1492 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Amakura,Y.,Yoshida,T. et al.: "Four new hyrolyzable tannins and acylated flavonol glycosides from Euphorbia maculata"Can.J.Chem.. 75. 727-733 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyake,Y.,Ito,H.,Yoshida,T. et al.: "Identification of iridals as piscicidal components of iridaceous plants and their conformations associated with CD spectra"J.Nat.Prod.. 75. 734-741 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Amakura,Y.,Ito,H.,Yoshida,T. et al: "Acalyphidins M_1, M_2, and D_1, Ellagitannins from Acalypha hispida"Phytochemsitry. 50・4. 667-675 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito,H.,Onoue,S.,Miyake,Y.and Yoshida,T.: "Iridal-Type Triterpenoids with Ichthyotoxic Activity from Belamcanda chinensis"J.Nat.Prod.. 62・1. 89-93 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito,H.,Miki,K.and Yoshida,T.: "Elaeagnatins A-G, C-glucosidic ellagitannins from Elaeagnus umbellata"Chem.Pharm.Bull.. 47・4. 536-542 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jose H.Isaza,M.,Ito,H.,Yoshida,T.: "Monochaetin, A Di-hyperin Ester of Tetrahydroxy-μ-truxinic Acid From Monochaetum multiflorum."Chem.Pharm.Bull.. 47・10. 1510-1511 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito,H.,Hatano,T.,Yoshida,T., et al.: "Modified Dehydroellagitannins, Geraniinic acids B and C, and Phyllanthusiin F."Chem.Pharm.Bull.. 47・8. 1148-1151 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jin,Z-X.,Ito,H.and Yoshida,T.: "Ellagitannin dimer and trimer from Corylus heterophylla"Phytochemistry. 48. 333-338 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida,T.,Hirai,Y.,Hayashi,S.,Hatano,T.,Ito,H.: "Anti-Helicobacter pylori Activity of Tannins and Related Polyphenols."Polyphenols Commun 2000. 1. 309-310 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taniguchi,S.,Ito,H.,Hatano,T.,Yoshida,T. et al.: "Galloylglucoses and riccionidin A in Rhus javanica Adventitious root cultures."Phytochemistry.. 53・3. 357-363 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida,T.,Hatano,T.,Ito,H.: "Chemistry and Fuction of Vegetable Polyphenols with High Molecular Weights."BioFactors. 13. 121-125 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida,T. et al.: "Plant Polyphenols 2"Kluwer academic/Plenum Publishers. 926 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatano, T., Ito, H., Yoshida, T.et al.: "Phenolic Constituents of Licorice.VII.A New Chalcone with a Potent Radical Scavenging Activity and Accompanying Phenolics from Liquorice."Chem.Pharm.Bull.. 45. 1485-1492 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amakura, Y., Yoshida, T.et al.: "Four new hyrolyzable tannins and acylated flavonol glycosides from Euphorbia maculata."Can.J.Chem.. 75. 727-733 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyake, Y., Ito, H., Yoshida, T.: "Identification of iridals as piscicidal components of iridaceous plants and their conformation associated with CD spectra."J.Nat.Prod.. 75. 734-741 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amakura, Y., Ito, H., Yoshida, T.et al.: "Acalyphidins M_1, M_2, and D_1, Ellagitannins from Acalypha hispida"Phytochemistry. 50(4). 667-675 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, H., Onoue, S., Miyake, Y.and Yoshida, T.: "Iridal-Type Triterpenoids with Ichthyotoxic Activity from Belamcanda chinensis"J.Nat.Prod.. 62(1). 89-93 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, H., Miki, K.and Yoshida, T.: "Elaeagnatins A-G, C-glucosidic ellagitannins from Elaeagnus umbellata."Chem.Pharm.Bull.. 47(4). 536-542 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jose H.Isaza, M., Ito, H., and Yoshida, T.: "Monochaetin, A Di-hyperin Ester of Tetrahydroxy-μ-truxinic Acid From Monochaetum multiflorum."Chem.Pharm.Bull.. 47(10). 1510-1511 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, H., Hatano, T., Yoshida, T., et al.: "Constituents of Geranium thunbergii Sieb.et Zucc.XV.Modified Dehydroellagitannins, Geraniinic acids B and C, and Phyllanthusiin F."Chem.Pharm.Bull.. 47(8). 1148-1151 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jin, Z-X., Ito, H.and Yoshida, T.: "Ellagitannin dimer and trimer from Corylus heterophylla"Phytochemistry. 48. 333-338 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, T., Hirai, Y., Hayashi, S., Hatano, T., Ito, H.: "Anti-Helicobacter pylori Activity of Tannins and Related Polyphenols."Polyphenols Commun 2000. Vol.1. 309-310 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi, S., Ito, H., Hatano, T., Yoshida, T.et al.: "Galloylglucoses and riccionidin A in Rhus javanica Adventitious root cultures."Phytochemistry. 53(3). 357-363 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, T., Hatano, T., Ito, H.: "Chemistry and Fuction of Vegetable Polyphenols with High Molecular Weights."Bio Factors. 13. 121-125 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanoh, R., Hatano, T., Ito, H., Yoshida, T.et al.: "Effect of tannins and related polyphenols on superoxide-induced histamine release from rat peritoneal mast cells."Phytomedicine. 7. 297-302 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gross G.G.et al.: "Kluwer Academic/Plenum Publishers"Plant Polyphenols 2. 926 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2002-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi