1999 Fiscal Year Final Research Report Summary
Molecular mechanisms of negative regulation on gene expression.
| Project/Area Number |
09672227
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
IMAGAWA Masayoshi Grad. Sch. of Pharm. Sci., Osaka University Assoc. Prof., 薬学研究科, 助教授 (20136823)
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| Co-Investigator(Kenkyū-buntansha) |
今川 正良 大阪大学, 薬学研究科, 助教授 (20136823)
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| Project Period (FY) |
1997 – 1999
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| Keywords | transcription / gene expression / silencer / DNA-binding protein |
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| Research Abstract |
The expression of glutathione transferase-P (GST-P) gene is rarely detected in normal liver, whereas it is highly induced during hepatocarcinogenesis of rat. We identified the silencer region in the GST-P promoter, and isolated the silencer binding proteins. In this project, we characterized the silencer binding proteins and mapped the functional (repressive) domains.
1) We cloned nuclear factor-1 (NF1) as a silencer binding protein, and NF1-A had a repressive activity. The minimal repression domain was also identified.
2) We cloned the genomic clone of NF1-A and identified the all exon-intron boundaries.
3) The splicing isoforms of NF1-B was isolated, and these proteins were found to function as both positive and negative transcription factors.
4) We characterized the DNA-binding specificities and affinities of NF1-A, -B, -C and -X.
5) The nuclear localization signals in the NF1-A were identified.
6) C/EBPδgene is regulated through C/EBP binding sites at the downstream region by autoregulation mechanism.
7) The novel silencer binding proteins were isolated by yeast one-hybrid system
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[Publications] Osada, S., Daimon, S., lkeda, T., Nishihara, T., Yano, K., Yamasaki, M., and lmagawa, M.: "Nuclear factor 1 family proteins bind to the silencer element in the rat glutathione transferase gene."J. Biochem.. 121. 355-363 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Osada, S., Matsubara, T., Daimon, S., Terazu, Y., Xu, M., Nishihara, T., and lmagawa, M.: "Expression, DNA-binding specificity and transcriptional regulation of nuclear factor 1 family proteins from rat."Biochem. J.. 342. 189-198 (1999)
Description
「研究成果報告書概要(欧文)」より
