1998 Fiscal Year Final Research Report Summary
Effects of Docosahexaenoic Acids on Vascular Cell Functions
| Project/Area Number |
09672244
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
HIRAFUJI Masahiko Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, 薬学部, 助教授 (20142987)
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| Co-Investigator(Kenkyū-buntansha) |
HAMAUE Naoya Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido Instr, 薬学部, 助手 (70221504)
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| Project Period (FY) |
1997 – 1998
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| Keywords | docosahexaenoic acid / vascular smooth muscle cells / intracellular calcium / nitric oxide / calcium channels / 5-hydroxytryptamine / angiotensin II |
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| Research Abstract |
The aim of the present project was to investigate the effects of docosahexaenoic acids (DHA) on vascular cells functions, using cultured aortic smooth muscle cells. Treatment of rat aortic smooth muscle cells with DHA in culture had no significant effect on the cell growth rate and cell hypertrophy induced by angiotensin II.The effects of DHA on intracellular C^<2+> dynamics and nitric oxide (NO) production were also examined using cells isolated from stroke-prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar Kyoto rats (WKY). The resting and peak concentrations of intracellular C^<2+> were significantly higher in cells from SHRSP than in WKY stimulated with angiotensin II or 5-hydroxytryptamine (5-HT). DHA treatment significantly suppressed the intracellular C^<2+> dynamics induced by angiotensin II and 5-HT in cells from WKY, while the cells from SHRSP were refractory to DHA treatment. It was suggested that DHA inhibited C^<2+> influx through the voltage-dependent o
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r 2nd messenger-operated C^<2+> channels, but not store-operated channels. The total NO production induced by interleukin-1beta (IL-1beta) was significantly lower in cells from SHRSP than from WKY.DI-LA treatment significantly increased IL-l3b-induced NO production in cells from WKY, while the cells from SHRSP were refractory to DHA treatment. The increase in NO production was due to the stimulation of inducible NO synthase protein induction, as analyzed by Western bloffing method. It is also suggested that DHA treatment can prevent cell damage evoked by IL-lbeta-induced NO.These studies suggest that the altered intracellular C^<2+> dynamics induced by contractile agonists and the diminished NO production in vascular smooth muscle cells play a role in the pathophysiology of SHRSP.Since the intracellular Ca and NO plays an important role in vascular smooth muscle cell functions, the properties to improve these functions can be contributed to the beneficial effect of DHA on cardiovascular disorders. Less
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Research Products
(16 results)
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[Publications] Hirafuji, M., Ebihara, T., Kawahara, F., Kanai, Y., Kato, K., Tanimura, A., Saito, H.and Minami, M.: "Antihypertensive mechanisms of docosahexaenoic acid(DHA)-Influences on vascular smooth muscle cells in culture." Jpn.Heart J. 38(4). 568 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hirafuji, M., Ebihara, T., Kawahara, F., Tsunoda, M., Hamaue, N., Tanimura, A.and Minami, M.: "Specific modulation of vascular smooth muscle cell functions by docosahexaenoic acid(DHA)" Folia Pharmacol.Jpn.110, Suppl1. 171-176 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hirafuji, M., Kanai, Y., Tsunoda, M., Ebihara, T., Kawahara, F.and Minami, M.: "Suppressive effect of serotonin on nitric oxide production by vascular smooth muscle cells isolated from stroke-prone spontaneously hypertensive rats." Jpn.Heart J. 39(4). 560 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hirafuji, M., Nezu, A., Kanai, Y., Ebihara, T., Kawahara, F., Tanimura, A.and Minami, M.: "Effect of 5-hydroxytryptamine on intracellular calcium dynamics in cultured rat vascular smooth muscle cells." Res.Commun.Mol.Pathol.Pharmacol.99(3). 305-319 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hirafuji, M., Ebihara, T., Kawahara, F., Nezu, A., Tanimura, A., H.Saito and Minami, M.: "Effect of docosahexaenoic acid on intracellular calcium dynamics in vascular smooth muscle cells from normotensive and genetically hypertensive rats." Res.Commun.Mol.Pathol.Pharmacol.102(1). 29-42 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hirafuji, M., Kanai, Y., Tsunoda, M., Ebihara, T., Kawahara, F.and Minami, M.: Nitric Oxide Production by Vascular Smooth Muscle Cells From Stroke-prone Spontaneously Hypertensive Rats, In, The Biology of Nitric Oxide, Part 6. Ed.by Moncada, S., Toda, N., Maeda, H.and Higgs, E.A., Portland Press, London and Minami, 153 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hirafuji, M., Minami, M., Ebihara, T., Kawahara, F., Yoshioka, M., Saito, H.and Parvez, H.S.: Changes in intracellular Ca2+ signaling induced by docosahexaenoic acid in vascular smooth muscle cells from a genetical hypertensive rat(SHRSP), In Progress in Hypertension, Vol.4. Ed.by Parvez, S.H., Frossard, P.M., Minami, M., Parvez, S.and Saito, H., VSP BV,Utrecht, The Netherlands(in press), (1999)
Description
「研究成果報告書概要(欧文)」より
