Does general anesthetics cause membrane fluidity?

1998 Fiscal Year Final Research Report Summary

• Project/Area Number: 09672339
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 応用薬理学・医療系薬学
• Research Institution: Toho University
• Principal Investigator: MOMOSE Yasunori Toho University, Department of Clinical Pfarmacy, Professor, 薬学部, 教授 (50020813)
• Project Period (FY): 1997 – 1998
• Keywords: General anesthetics / Membrane fluidity / Ionic Channel / Cell membrane / 膜容量

Research Abstract

We studied the general anesthetics on membrane capacitance and L-type of Ca^<2+> channel currents in single cardiac myocytes from guinea-pig ventricle. Membrane capacitance (tau)measured by electrophysiological techniques. Halothene, isoflurane and sevoflurane slightly decreased membrane capacitance in single ventricular myocytes. However, thiopental and propofol increased membrane capacitance. Mode of Ca^<2+> channel inhibition by hailothane, isoflurane and sevoflurane are non-use-dependent block, but Thiopental and propofol inhibited Ca^<2+> channel is use-dependent block actions. The results showed that the mechanism of intravenous and inhalation anesthetics action are differ from membrane capacitance. Thiopental and propofol inhibited Ca^<2+> channel through membrane fluidity, however, halothane and isoflurane did not through membrane fluidity.