1999 Fiscal Year Final Research Report Summary
The mechanism for synthesis and secretion of apo A-IV in CaCo-2 cells
| Project/Area Number |
09672353
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Gifu University School of Medicine |
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Principal Investigator |
SEISHIMA Mitsuru Department of Laboratory Medicine, Gifu University School of Medicine, Professor, 医学部, 教授 (10171315)
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| Project Period (FY) |
1997 – 1999
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| Keywords | Apo A-IV / Fatty acids / CaCo-2 cell |
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| Research Abstract |
Human apo A-IV is an unique apolipoprotein which is synthesized only in enterocytes and is involved in lipid absorption. However, the mechanism for synthesis and secretion of apo A-IV has not been fully explored. In this study, the effect of two kinds of fatty acid, oleic (CィイD218:1ィエD2) and caprylic (CィイD28:0ィエD2) acids, on apo A-IV synthesis and sedretion was examined in CaCo-2 cells. CaCo-2 cells, which are cell line origenated from human colon cancer cells and show characteristics of intestinal epithelim, were cultured on the filter mambrane of Transwell for 6 hr at 37℃ with 3.0 mM of oleic or caprylic acid with a micellar form. Oleic acid increased triglyceride secretion by 4 times, whereas caprylic acid increased by 1.3 times, compared to control. Apo A-IV concentration in the medium was significantly decreased in oleic acid, compared to caprylic acid. In contrast, apo A-IV mRNA level in cells is significantly increased in oleic acid compared to caprylic acid. It is suggested that caprylic acid enhances the secretion of apo A-IV without the increase in synthesis and oleic acid increases the transcription rate of apo A-IV, at least in CaCo-2 cells. These different effects of fatty acids on apo A-IV depends on the different carbon number of fatty acids.
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