• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1999 Fiscal Year Final Research Report Summary

The relationship between Pathology and Unbalance of Fas isoforms

Research Project

Project/Area Number 09672360
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionNagasaki University

Principal Investigator

KAMIHIRA Shimeru  Laboratory Medicine, Nagasaki University, Professor, 医学部, 教授 (80108290)

Co-Investigator(Kenkyū-buntansha) MAEDA Takahiro  Dept. of Hematology, Nagasaki University, Assistant Professor, 医学部, 助手 (40284674)
YAMADA Yasuaki  Laboratory Medicine, Nagasaki University, Associate Professor, 医学部, 助教授 (60145232)
Project Period (FY) 1997 – 1999
KeywordsFas / isoform / ATL / HTLV-I / tumorigenesis / Apoptosis / Fas ligand / mutation
Research Abstract

The Fas-Fas ligand signal transduction system is well known to be involved in not only normal development but also incurable disorders, such as neoplasms and immune-diseases. There are possible three factors in terms of tumorigensis via Fas receptor; 1) down-regulation of membrane (m) Fas to escape from tumor elimination, 2)dominat negative action due to mutated Fas, and 3)inhibitory or antagonistic function by isoform of soluble(s) Fas. To address each issue described above, we studied the relationship between expression of aberrant Fas isoforms and ATL cell biology. First of all, quantitative flow cytometric assay revealed that 90% ATL had tumor cells up-regulating mFas, while l0% ATL had cells negative for mFas with no mutated Fas genes (Br J Haematol 99;858,1997). Secondly, we encountered a case with mFas positive ATL cells of which no signal transduction via Fas receptor was evident, Molecular analysis on these cells revealed mutated Fas deleting 20bp at exon 9 and resulting in truncated Fas proteins. This truncated Fas was evident to serve as dominant negative function in the experimental system of Jurkat cell line transfected with the gene (J Exp Med 189:1063,1999). Thirdly, our study also disclosed a possible mechanism which over-productive sFas acts as decoy function to prevent from cell death via the Fas-FasL signal system (Br J Haematol 107;851, 1999).

  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Shigeki Takemoto: "proliferation of adult T cell leukemia / lymphoma cells is associated with the constitutive activation & JAK/STAT proteins"Proc. Natl. Acad. Sci.. 94. 13897-13902 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuaki Yamada: "Frequent Hepatic Involvement in Adult T Cell leukemia : Comparison with non-Hodgkin's Lymphoma"Leukemia and Lymphoma. 26. 327-335 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuaki Yamada: "Delections of p15 and / or p16 as a Poor-Prognosis Factor in Adult T-Cell Leukemia"Journal of Clinical Oncology. 15. 1778-1785 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuyuki Sugahara: "Soluble and membrane isoforms of Fas / CD95 in fresh adult T-Cell Leukemia (ATL) Cells and ATL-Cell Lines"Int. J. cancer. 72. 128-132 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimeru Kamihira: "Serum Levels of Soluble Fas / APT-1 Receptor in HumanRetroviral Infection and Associated Diseases"Internal Medicine. 36(3). 166-170 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimeru Kamihira: "Quantitative characterization and potential function of memnrane Fas / APC-1 (CD95) receptors on leukaemic cells from chronic band T Lymphoid Leukaemias"British Journal of haematology. 99. 858-865 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuko hori: "The Methylthicadenosine phosphorylase Gene is Frequently CO-Deleted with the p16^<INK4a> Gene in Acute Type Adult T-Cell Leukemia"Int. J. Cancer. 75. 51-56 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kunihiro Tsukasaki: "Possible Association betweenAdult T-Cell Leukemia / Lymphoma and acute Myeloid Leukemia"Cancer. 82(3). 488-494 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masaomi Yamamura: "Circulating interleukin-6 levels are elevated in adult T-Cell leukemia/lymphoma patients and correlate with adverse clinical features and survival"British Journal of Haematology. 100. 129-134 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuaki Yamada: "Interleukin-15(IL-15) Can Replace the IL-2 Signal in IL-2-Dependent Adult T-Cell Leukemia (ATL) Cell Lines: Expression of IL-15 Receptor α on ATL Cells"Blood. 91(11). 4265-4272 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoshi Fujimura: "Retinoic acids induce growth inhibition and apoptosis in adult T-Cell leukemia (ATL) cell lines"Leukemia Research. 22. 611-618 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihiro Hotta: "Allelotype Analysis of Adult T-Cell Leukemia"Blood. 92(6). 2113-2117 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Hayashibara: "Vascular endothelial factor at high plasma levels is associated with extranodal involvement in adult T cell leukemia patients"Leukemia. 13(10). 1634-1635 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimeru Kamihira: "Discrepant expression of memnrane and soluble isoforms of Fas (CD95/APC-1) in adult T-cell leukemia : soluble Fas isoform is an independent risk factor for prognosis"British Journal of Haematology. 107. 851-860 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuaki Yamada: "Pathological Roles of Interleukin-15 inAdult T-Cell Leukemia"Leukemia and Lymphoma. 35(1-2). 37-45 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koki Ikeda: "Adult T-cell Leukemia cells Over-Express the Multidrug-Resistance-protein (LRP) Genes"Int. J. cancer. 82. 599-604 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tsukasaki: "Diversity of leukaemic cell morphology in ATL correlates with prognostic factors aberrant immunophenotype and defective HILV-1 genotype"British Journal Haematology. 105(2). 369-375 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahiro Maeda: "Fas Gene Mutation in the Progression of Adult T-Cell Leukemia"J. Exp. Med. 189(7). 1063-1071 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeki Takemoto: "Proliferation of adult T cell leukemia/lymphoma cells is associated with the constitutive activation of JAK/STAT proteins"Proc. Natl. Acad. Sci. USA. 94. 13897-13902 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuaki Yamada: "Frequent Hepatic Involvement in Adult T Cell Leukemia: Comparison with non-Hodgkin's Lymphoma"Leukemia and Lymphoma. 26. 327-335 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuaki Yamada: "Deletions of p15 and/or p16 Genes as a Poor-Prognosis Factor in Adult T-Cell Leukemia, Journal of Clinical Oncology"Journal of Clinical Oncology. 15. 1778-1785 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuyuki Sugahara: "Soluble and membrane isoforms of Fas/CD95 in fresh adult T-Cell Leukemia(ATL) Cells and ATL-Cell Lines"Int. J. Cancer. 72. 128-132 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimeru Kamihira: "Serum Levels of Soluble Fas/APT-1 Receptor in Human Retroviral Infection and Associated Diseases"Internal Medicine. 36(3). 166-170 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kamihira: "Quantitatige characterization and potential function of membrane Fas/APO-1(CD95) receptors on leukaemic cells from chronic B and T lymphoid leukemias"British Journal of Haematology. 99. 858-865 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuko Hori: "The Methylthioadenosine phosphorylase gene is frequently co-deleted with the p16INK4a gene in acute type adult T-cell leukemia"Int. J. Cancer. 75. 51-56 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunihiro Tsukasaki: "Possible Association between Adult T-Cell Leukemia/Lymphoma and Acute Myeloid Leukemia"Cancer. 82. 488-494 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masaomi Yamamura: "Circulation interleukin-6 levels are elevated in adult T-celll leukaemia/lymphoma patients and correlate with adverse clinical features and survival"British Journal of Haematology. 100. 129-134 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuaki Yamada: "Interleukin-15(IL-15)Can Replase the IL-2-Dependent adult T-Cell Leukemia(ATL Cell Lines: Expression of IL-15 Receptor α on ATL Cells"Blood. 91(11). 4265-4272 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoshi Fujumura: "Retinoic acids induce growth inhibition and apoptosis in adult T-cell leukemia (ATL) cell lines"Leukemia Research. 22. 611-618 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshihiro Hatta, et al.: "Allelotype Analysis of Adult T-Cell Leukemia"Blood. 92(6). 2113-2117 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T Hayashibara: "Vascular endothelial growth factor at high plasma levels is associated with extranodal involvement in adult T-cell leukemia patients"Leukemia. 13(10). 1634-1635 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimeru Kamihira: "Discrepant expressing of membrane and soluble isoforms of Fas (CD95/APO-1) in adult T-cell leukaemia: soluble Fas isoform is an independent risk factor for prognosis"British Journal of Haematology. 107. 851-860 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuaki Yamada: "Pathological Roles of Interleukin-15 in Adult T-Cell Leukemia."Leukemia and Lymphoma. 35(1-2). 37-45 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koki Ikeda: "Adult T-Cell Leukemia Cells Over-Express The Multidrug-Resistance Protein(MRP) and Long-Resistance-Protein (LRP) Genes"Int. J. Cancer. 82. 599-604 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K Tsukasaki: "Diversity of leukaemic cell morphology in ATL correlates with prognostic factors, aberrant immunophenotype and defective HTLV-1 genotype"Br J Haemato.. 105(2). 369-375 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahiro Maeda: "Fas Gene Mutation in the Progression of Adult T Cell Leukemia."J Exp Med. 189(7). 1063-1071 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2001-10-23  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi