1998 Fiscal Year Final Research Report Summary
Analysis of the Mechanisms of Complement Cold Activation of the Serum from C-type Hepatitis Patients
| Project/Area Number |
09672361
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Fukushima Medical College |
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Principal Investigator |
NAGAI Toshihiko Dept. of Medicine, Fukushima Medical College, Assis. Prof., 医学部, 助手 (90180447)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Hiroshi Dept. of Medicine, Fukushima Medical College, Prof., 医学部, 教授 (90045683)
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| Project Period (FY) |
1997 – 1998
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| Keywords | C-type hepatitis / cold activation / complement / 補体価 / 補体活性化 |
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| Research Abstract |
Cold activation of complement (CA) of the serum from patients with C-type hepatitis was partially prevented by adding alpha 2-plasmin inhibitor (PI) in a dose dependent manner. Tranexamic acid also showed week effect but bdelin and urinastatin did not. Intrinsic activities of PI in such patient sera were significantly lower than those of normals and the PI activities of normal sera were decreased by cold incubation. No significant difference was observed between the factor VII activity of patients and that of normals. CA was induced to healthy sera by the addition of high molecular weight fractions (>〜100kd) from CA positive sera prepared by gel filtration. High molecular weight fractions prepared by ultrafiltration also caused CA on healthy sera in the dose dependent manner and the CA inducing activity was remained even after CA occurred. Cryo-globurin obtained from patient sera induced CA but some cryo-globurin negative sera also showed CA.
From these results it is speculated that the decrease of intrinsic plasmin inhibitors, like alpha2-PI, and the activation of plasmin like enzyme(s) in cold incubation is one of the factors of cold complement activation. It was also suggested that CA inducing high molecular factors other than cryo-globurin is present in such patient sera.
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