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1998 Fiscal Year Final Research Report Summary

Analysis of the Mechanisms of Complement Cold Activation of the Serum from C-type Hepatitis Patients

Research Project

Project/Area Number 09672361
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionFukushima Medical College

Principal Investigator

NAGAI Toshihiko  Dept. of Medicine, Fukushima Medical College, Assis. Prof., 医学部, 助手 (90180447)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Hiroshi  Dept. of Medicine, Fukushima Medical College, Prof., 医学部, 教授 (90045683)
Project Period (FY) 1997 – 1998
KeywordsC-type hepatitis / cold activation / complement / 補体価 / 補体活性化
Research Abstract

Cold activation of complement (CA) of the serum from patients with C-type hepatitis was partially prevented by adding alpha 2-plasmin inhibitor (PI) in a dose dependent manner. Tranexamic acid also showed week effect but bdelin and urinastatin did not. Intrinsic activities of PI in such patient sera were significantly lower than those of normals and the PI activities of normal sera were decreased by cold incubation. No significant difference was observed between the factor VII activity of patients and that of normals. CA was induced to healthy sera by the addition of high molecular weight fractions (>〜100kd) from CA positive sera prepared by gel filtration. High molecular weight fractions prepared by ultrafiltration also caused CA on healthy sera in the dose dependent manner and the CA inducing activity was remained even after CA occurred. Cryo-globurin obtained from patient sera induced CA but some cryo-globurin negative sera also showed CA.
From these results it is speculated that the decrease of intrinsic plasmin inhibitors, like alpha2-PI, and the activation of plasmin like enzyme(s) in cold incubation is one of the factors of cold complement activation. It was also suggested that CA inducing high molecular factors other than cryo-globurin is present in such patient sera.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Toshihiko Nagai: "Analysis of the factors inducing cold activation of complent in the serum from C-type hepatitis patient."The fifth asian conference of clinical pathology. Abs.. 61 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉田 浩: "血清補体溶血活性(CH50)の測定と検体取り扱い上の問題"臨床病理. 特集103. 221-226 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 長井 俊彦: "補体Cold Activation に関与する因子の解析とその機構について"臨床病理. 補冊45. 193 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Nagai et al.: "Analysis of the Factors Inducing Cold Activation of Complement in the Serum from C-type Hepatitis patients"The 5th ACCP. 61 abstracts. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Yoshida et al.: "Measurements of Serum Complement Activity and its Sampling"Jpn. J. Clin. Pathol.. 103. 221-226 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Nagai et al.: "Analysis of the Factors of Complement Cold Activation and Its Mechanism"Jpn. J. Clin. Pathol.. 45 Abs.. 193 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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