1998 Fiscal Year Final Research Report Summary
Mechanism of enhancing vitamin E activity by sesame seed or its lignans
| Project/Area Number |
09680043
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
家政学
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| Research Institution | Sugiyama Jogakuen University |
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Principal Investigator |
YAMASHITA Kanae Sugiyama Jogakuen University, Professor, 生活科学部, 教授 (40080113)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Siko Sugiyama Jogakuen University, Assistant, 生活科学部, 助手 (80308808)
OSAWA Toshihiko Nagoya University, Professor, 農学部, 教授 (00115536)
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| Project Period (FY) |
1997 – 1998
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| Keywords | sesame seed / sesamin / sesaminol / alpha-tocopheol / gamma-tocopherol / alpha-tocopherol transfer protein / rat |
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| Research Abstract |
Mechanisms enhancing vitamin E activity by sesame seed or its lignans were investigated in three experiments.
In experiment 1 we examined the concentration of the liver alpha-tocopherol transfer protein (alphaTTP) in the liver of rats fed a control diet or a 20% sesame seed containing diet. The concentration was evaluated by the immuno-antibody method using immuno-antibodies kindly donated by Dr. Arai (Dept. of Pharmacology, Tokyo University). The results showed no difference in the concentrations of alphaTTP between control rats and sesame seed fed rats.
In experiment 2 we investigated the suppressing effect of sesame lignans (sesamin and sesaminol) on lipid peroxides produced by 0.5 % DHA feeding in rats. In rats fed DHA, lipid peroxide levels significantly increased in the plasma, liver and brain, and alpha-tocopherol levels decreased in the plasma and liver but not the brain. The addition of sesamin and sesaminol significantly inhibited production of lipidperoxide and raised alpha-tocopherol levels in the plasma, liver and brain. These effects were nearly the same with either sesamin or sesaminol.
In experiment 3, gamma-tocopherol concentration in the brain of rats fed sesame seed, which contains only g-tocopherol, was compared to that of rats fed gamma-tocopherol. A high gamma-tocopherol concentration was found in tha sesame seed fed group, but negligible amounts of gamma-tocopherol in brain were found in the gamma-tocopherol-fed group. Further, the concentration of alpha-tocopherol in the brain of rats fed sesame seed with alpha-tocopherol was higher than that of control group without sesame seed.
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