1998 Fiscal Year Final Research Report Summary
Studies on cytokines and proteases which regulate UV-induced mutation in human cells
| Project/Area Number |
09680516
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | Chiba University |
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Principal Investigator |
SUZUKI Nobuo Chiba Univ.Med.Biochemi., Professor, 医学部, 教授 (90111426)
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| Co-Investigator(Kenkyū-buntansha) |
HIWASA Takaki Chiba Univ.Med.Biochemi., Assistant, 医学部, 助手 (30260251)
ISHIJIMA Sumio Kyoto Pref.Univ.Dept.Agric., Assoc.Professor, 農学部, 助教授 (70184520)
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| Project Period (FY) |
1997 – 1998
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| Keywords | human cells / ultraviolet ray / mutation / cytokine / protease |
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| Research Abstract |
In order to clarify molecular mechanisms which regulate mutability in UV(mainly 250nm wavelength) - irradiated human cells, particularly those regulated by proteases and cytokines.
1. Partial purification and characterization of UV-induced proteases were preformed and protease samples were obtaind from cell lysates of variant cells with increased levels of the induced protease activity. The samples contained molecules with about 20,000 Mr, the activity of which is inhibited by antipain but enhanced by ALLNa1.
2. Using PCR-differential display, we have searched for genes expressed specifically in human interferon (HuIFN)- beta -treated Cockayne syndrome (CS) fibroblast cells. The cDNAs of six clones were similar to expression tagged sequences from unknown genes in databases and the remaining one was identical to the cDNA of the xeroderma pigmentosum XPG gene. Together with our previous finding of increased resistance to ultraviolet (UV) cell-killing of CS cells pretreated with HuIFN- beta
… More
prior to UV irradiation, suggest that XPG might be one of the genes possibly involved in the HuIFN- beta-induced UV-resistance.
3. UV-resistant UV'-10 cells were established from UV-sensitive human RSb cells. We searched here for genes expressed differentially between UV'-10 and RSb cells using a differencial display method to elucidate the molecular mechanisms underlying the UV-resistance. Thirteen candidate cDNA fragment were obtained from 514 mRNA species first screened. Among the cDNA fragments, 3 revealed increased mRNA expression in UV'-10 cells compared with RSb cells by Northern analysis. Nucleotide sequence analysis identified 2 of the 3 cDNA fragments as encoding nucleophosimin (NPM) and T-plastin. The expression of NPM mRNA was induced after UV irradiation in UV'-10 cells but not in RSb cells, whereas irradiation did not affect the expression of T-plastin mRNA.UV'-10 cells transfected with antisense cDNA for NPM mRNA and possibly T-plastin genes may contribute to the incresed resistance to UV cell-killing, at least in the human cells tested. Less
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Research Products
(21 results)
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[Publications] Fujii, K., Suzuki, N., Kita, K., Ishijima, S,Sonoda, T., Dezawa, M., Sugita, K.and Niimi, H.: "Abnormal DNA synthesis activity induced by X-rays in nevoid basal cell carcinoma syndrome cells." Biochem.Biophys.Res.Commun.240. 269-272 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Isogai, E,Ishijima, S., Sonoda, T., Kita, K., Suzuki, H., Hasegawa, R., Yamamori, H., Takakubo, Y., and Suzuki, N.: "Protease activation following UV irradiation is linked to hypomutability in human calls selected for resistance to combination of UV and antipain" Mutation Res.403. 215-222 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sugita, K,Suzuki, N., Higuchi, Y., Kita, K., Suzuki, Y., and Lehmann, A.: "Enhancement of XP-G mRNA expression by human interferon-beta in Cockayne syndrome cells." Mutation Res.408. 67-72 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Suzuki, H,Watanabe, M., Wu, Y., Sugita, T., Kita, K., Sato, T., Wang, X., Tanzawa, H., Sekiya, S.and Suzuki, N.: "Mutagenicity of microcystin-LR in human RSa cells." Int.J.Mol.Med.2. 109-112 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Higuchi, Y,Kita, K., Nakanishi, H., Wang, XL., Sugaya, S., Tanzawa, H., Yamamori, H., Sugita, K., Yamamura A.and Suzuki, N.: "Search for genes involved in UV-resistance in human cells by mRNA differential display : increased transcriptional expression of nucleophosmin and T-plastin genes in association with the resistance." Biochem.Biophys.Res.Commun.248. 597-602 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Suzuki, N., Ishibashi, M., Kita, K., Wu, Y., Nomura, J., Takakubo, Y., Hiroshima, K., Genga, K., Ohwada, H.and Hayashi, Y.: "Detection of Serum Factors Enhancing Cell Mutability From Lung Cancer Patients by Application of Hypermutable Human RS Cells." Int.J.Cancer. 78. 550-555 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Wu, Y., Hiwasa, T,Isogai, E., Sonoda, T., Kita, K., Chen, Z., Sugaya, S., Yamamori, H., Takazawa, H.and Suzuki, N.: "Activation of MAP kinases by 5-fluorouracil in a 5-fluorouracil-resistant variant human cell line derived from a KT breast cancer cell line" Int.J.Oncol.13. 1241-1245 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kikuno, K., Kita, K., Nomura, J., Hiwasa, T., Yonemitsu, H.and Suzuki, N.: "Search for genes responsible for UV susceptibility of human cells : involvment of syndecan-1 in UV resistance." Biochem.Biophys.Res.Commun.253. 519-523 (1998)
Description
「研究成果報告書概要(欧文)」より
