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1998 Fiscal Year Final Research Report Summary

Molecular mechanism of radioadaptive response

Research Project

Project/Area Number 09680520
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionKyoto University of Education

Principal Investigator

IKUSHIMA Takaji  Kyoto University of Education, Department of Science, Professor, 教育学部 (80027458)

Project Period (FY) 1997 – 1998
Keywordsradiation / adaptive response / DNA repair
Research Abstract

The radioadaptive response is the phenomenon that cells pre-exposed to low doses of ionizing radiation can acquire the resistance to subsequent moderate or high doses of radiation for Induction of chromosomal aberrations, mutation and cell killing. To elucidate the molecular mechanism of radloadaptive response, a series of researches has been carried out In cultured mammalian cells which was previously proved to show cytogenetical adapative response.
A hypothesis that the Induction of a novel, efficient repair mechanism for chromosomal DNA damage may be involved In the radioadaptive response was tested by a neutral comet assay of repair kinetics of radiation-induced DNA damage in adapted Chinese hamster V79 cells. There were no indication of any difference In the initial yields of DNA damage (double-strand breaks) induced by challenging doses between non-adapted cells and cells adapted by priming exposure. The monitoring of the rejoining of DNA double-strand breaks induced by challenging doses has revealed that the rate of DNA damage repair in adapted cells was higher than that in non-adapted control cells, and the level of the residual damage was less In adapted cells. The enhanced repair rate was observed especially In the early stage of DNA repair process.
A cross-adaptive response was also demonstrated for enhanced repair of DNA damage induced by a radiomimetic agent, neocarzinostatin in radio-adapted cells. These results indicate that the radioadaptive response may result from the induction of a novel, efficient DNA repair mechanism which leads to less residual damage, but not from the induction of protective functions that reduce the initial damage, It Is suggested that the radioadaptive response may not be restricted to ionizing radiation. These fruitful results obtained in this study would be helpful to elucidate the molecular mechnism of radioadaptive response in more detail.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] T.Ikushima, H.Aritomi & J.Morisita: "Radioadaptive response : Efficient repair of radiation-induced DNA damage in adapted cells." Mutation Research. 358. 193-198 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ikushima: "Molecular mechanism of radioadaptive response : A cross-adaptive response for enhanced repair of DNA damage in adapted cells" IAEA-TECDOC. 976. 112-115 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 生島 隆治: "放射線適応応答 : 現象から分子機構" 広島医学. 51. 281-283 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Hashiguchi & T.Ikushima: "Nucleotide changes in mitochondrial 16S rRNA gene from different mammalian cell lines" Gene and Genetic System. 73. 317-321 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ikushima, Y.Shima & Y.Ishii: "Effects of an inhibitor of topoisomerase II, ICRF-193 on the formation of ultraviolet-induced chromosomal aberrations" Mutation Research. 404. 35-38 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ikushima: "Radioadaptive response : responses to the five questions" BELLE Newsletter. 7. 14-15 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ikushima, H.Aritomi & J.Morisita: "Radioadaptive response : efficient repair of radiation-Induced DNA damage in adapted cells." Mutation Research. 358 (2). 193-198 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ikushima: "Molecular mechanism of redioadaptive response : a cross-adaptive response for enhanced repair of DNA damage in adapted cells." IAEA-TECDOC. 976. 112-115 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] 51 (3). 281-283

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Hashiguchi & T.Ikushima: "Nucleotide changes in mitochondrial 16S rRNA gene from different mammalian cell lines" Genes and Genetic Systems.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ikushima, Y.Shima & Y.Ishii: "Effects of an inhibitor of topoisomerase II,ICRF-193 on the formation of ultraviolet-induced chromosomal aberrations." Mutation Rsearch. 404 (1,2). 35-38 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ikushima: "Radioadaptive response : responses to the five questions." BELLE Newsletter. 7 (3).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ikushima: Sister chromatid exchanges : a dynamic behaviour of eukaryotic chromosomes. Trends in Environmental Mutagenesis (eds.R.C.Sobal et al.) Tausco, New Delhi, 91-100 (1999)

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      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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