1998 Fiscal Year Final Research Report Summary
An attempt to develop a biodosimeter for detecting low dose and low dose rate radiation
| Project/Area Number |
09680521
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | Osaka University |
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Principal Investigator |
NAKAJIMA Hiroo Osaka University Faculty of Medicine, Assistant Professor, 医学部, 助手 (20237275)
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| Co-Investigator(Kenkyū-buntansha) |
NOMURA Taisei Osaka University Faculty of Medicine, Professor, 医学部, 教授 (90089871)
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| Project Period (FY) |
1997 – 1998
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| Keywords | SCID / Cell culture / Mouse / Biodosimater / Double strand break / DNA repair / Low dose radiation / Potentially lethal damage |
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| Research Abstract |
We attempted to develop a new biodosimeter to detect the low dose and low dose rate radiation.
Normal (wild type cells) and double-strand break repair deficient murine cells (SCID cells) were exposed to gamma-rays at low and high dose rates with dose fractionation and with different recovery times, in order to examine the dose rate effectiveness and repair of sublethal and potentially lethal damage.In addition, 5-fluorouracil (5-FU), a commonly used drug for cancer therapy, was a]so used, because it produces double-strand breaks.
SOlD cells showed deficiency in repairing DNA damage induced by 5-FU or gamma-rays even after dose fractionation and after 24h recovery periods, while the wild type cells showed an apparent repair ability on DNA damage after 5-FU treatment or gamma-rays exposure.
We conclude that the SCID cell is useful system as a biodosimeter for detecting the damage of low dose radiation.
Now, we are trying to establish a method to count the number of double-strand break points in a SCID cell.
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