1998 Fiscal Year Final Research Report Summary
Investigation of DNA Binding Oligopeptide
| Project/Area Number |
09680568
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
NAKATANI Kazuhiko KYOTO UNIVERSITY,Graduate School of Engineering, Associate Professor, 工学研究科, 助教授 (70237303)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Photocrosslinking / oligopeptide / DNA |
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| Research Abstract |
We have examined synthesis and evaluation of novel oligopeptide that bound sequence selectively to duplex DNA.While Dervan and co-workers have been intensively studied pyrrole polyamide compounds as a sequence selective DNA binding peptide, there are no obvious general principle for molecular design for such peptides. In order to gain insight into the general principle for designing sequence selective DNA-binding peptide, we have studied the combinatorial approach, that exploiting fraction of photocross linking as a measure for the efficiency of DNA binding. As a result, we have used photoaffiniti group such as aryl azide and dibenzoyldiazomethane that we have previously developped for G-acylating agent, but we could not get the efficient cross linking between DNA and peptide. Due to a lack of the positive control for binding as well as cross linking, we could not assess our approach properly. But these results clearly pointed out that photocross linking is not suitable for this purpose, because cross linking efficiency is not high as we expected. In the future, we are going to examine other types of cross linking reaction such as metal-catalyzed reaction.
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