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1998 Fiscal Year Final Research Report Summary

Remodeling of regulation of cell growth by glycogene and its application.

Research Project

Project/Area Number 09680590
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

FUJII Junichi  Osaka University Medical School, Associate Professor, 医学部, 助教授 (00222258)

Co-Investigator(Kenkyū-buntansha) HIGASHIYAMA Shigeki  Osaka University Medical School, Assistant Professor, 医学部, 助手 (60202272)
Project Period (FY) 1997 – 1998
KeywordsApoptosis / Growth factor / Signal transduction / EGF receptor
Research Abstract

Although the importance of glycosylation of components on the cell membrane is widely accepted, the relationship with cell growth has been only poorly investigated.We remodeled the sugar chains on the cells by transfecting PC 12 cells with rat beta- 1, 4-N- acetlylglucosamyltransferase III gene (GnT III) and effects of them on the cellular responses to the nerve growth factor (NGF) were examined.The results show that branch structure, a product of the gene, was found in GnT III-transfected PC 12 cells.However, no change in the growth rate or neurites formation was observed.When effects of NGF on Trk, a NGF receptor, was investigated, tyrosine phosphotylation, which occurs in control cells, was not observed.Moreover, dimerization essential for signal transduction to nucleus did not occur.
Then we transfected B 16 melanoma, a line commonly used for metastasis, NIH3T3, and CHOK1 cells with EGF receptor and GnT III cDNAs by electroporation and selected neomycin-resistant cells.Several clones which highly express GnT III were isolated.The sugar chains of the EGF receptor was modified with bisecting GlcNAc structure and dysfunction of cellular response to EGF stimuli was observed.Currently we are investigating the MAP kinase cascade downstream of the EGF receptor signaling.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Asahi,M: "The oxidation of selenocysteine is involved in the inactivation of glutathione peroxidase by nitric oxide donor." J Biol Chem. 272. 19152-19157 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Miyazawa: "Immunological detection of fructated proteins in in vitro and in vivo." Biochem J. 336. 101-107 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Fujii: "A defect in the mitochondrial import of mutant Mn-superoxide dismutase produced in Sf21 cells." J Biochem. 124. 340-346 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Hayase: "Expression of heparin-binding epidermal growth factor-like growth factor in rat brain." Brain Res. 784. 163-178 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Yamaguchi: "Mapping of the α1,6fucosyltransferase gene,FUT8,to human chromosome 14q24.3." Cytogenet Cell Gent. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Miyazaki: "Gastrin induces heparin-binding epidermal growth factor-like growth factor in rat gastric epithelial cells transfected with gastrin receptor." Gastroenterology. 116. 78-89 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Fujii: "Pathophysiology of Lipid Peroxides and Related Free Radicals (K.Yagi ed.)" Japan Scientific Societies Press,Tokyo, 10 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Fujii: "Medical Biochemistry (Dominiczak MH,and Baynes JW eds.)" Mosby,London (in press), (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Asahi: "The oxidation of selenocysteine is involved in the inactivation of glutathione peroxidase by nitric oxide donor" J Biol Chem. 272. 19152-19157 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Miyazawa: "Immunological detection of fructated proteins in in vitro and in vivo." Biochem J. 336. 101-107 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Fujii: "A defect in the mitochondrial import of mutant Mn-superoxide dismutase produced in Sf21 cells." J Biochem. 124. 340-346 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Hayase: "Expression of heparin-binding epidermal growth factor-like growth factor in rat brain." Brain Res. 784. 163-178 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Yamaguchi: "Mapping of the a1,6fucosyltransferase gene, FUT8, to human chromosome 14q24.3." Cytogenet Cell Genet. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Miyazaki: "Gastrin induces heparin-binding epidermal growth factor-like growth factor in rat gastric epithelial cells transfected with gastrin receptor." Gastroenterology. 116. 78-89 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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