1998 Fiscal Year Final Research Report Summary
Membrane integration and intracellular localization of microsomal cytochrome P450
| Project/Area Number |
09680598
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
SAKAGUCHI Masao Graduate School of Medical Science, KYUSHU UNIVERSITY Associate Professor, 大学院・医学系研究科, 助教授 (30205736)
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| Project Period (FY) |
1997 – 1998
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| Keywords | endoplasmic reticulum / signal sequence / membrane topology / membrane protein / cytochrome P450 |
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| Research Abstract |
Using cytochrome P450 molecule as a typical model of endoplasmic reticulum membrane protein, we investigated its biosynthesis, integration into membrane, and localization in endoplasmic reticulum, and have demonstrated the follows :
(1) Type I signal-anchor sequence (SA-I) which is essential for correct folding of P450 molecule shows a key role of topogenic sequence during membrane integration of multispanning membrane proteins.
(2) For membrane integration of SA-I, a amino acid resides which induce turn structure are essential, when the amino-terminal domain is tend to form tight secondary structure.
(3)The length of hydrophobic segment of SA-I is one of the key determinants of intracellular location of ER.
(4) SA-I is integrated into the membrane just after the hydrophobic segment emerges from ribosomes.
(5) NADPH-cytochrome P450 reductase also possesses SA-I as P450 molecule does.
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[Publications] Makishima, T., Nakashima, T., Nagata-Kuno, K., Fukushima, K., Iida, H., Sakaguchi, M., Ikehara, Y., Komiyama, S.and Nishimoto, T.: ""The highly conserved DAD1 protein involved in apoptosis is required for N-linked glycosylation."" Genes to Cells. 2. 129-141 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Nakamura, N., Yamazaki, S., Sato, K., Nakano, A., Sakaguchi, M.and Mihara, K.: ""Identification of potential regulatory elements for the transport of Emp24."" Mol.Biol.Cell. 9. 3493-3503 (1998)
Description
「研究成果報告書概要(欧文)」より
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