1998 Fiscal Year Final Research Report Summary
The functions and their mechanisms of DNA-binding proteins, HMG1 and HMG2
| Project/Area Number |
09680601
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Science University of Tokyo, Department of Biological Science and Technology |
|---|
Principal Investigator |
YOSHIDA Michiteru Science University of Tokyo, Department of Biological Science and Technology, Professor, 基礎工学部, 教授 (20005648)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
UCHIYAMA Hidefumi Science University of Tokyo, Department of Biological Science and Technology, As, 基礎工学部, 助手 (40307707)
SHIRAKAWA Hitoshi Science University of Tokyo, Department of Biological Science and Technology, As, 基礎工学部, 助手 (40206280)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Keywords | HMG proteins / nuclear chromosomal protein / HMG1 / HMG2 / chromatin structure / DNA-protein interaction |
|---|
| Research Abstract |
(1) The mechanism of DNA-binding of HMG 1/2 proteins and the accompanied changes in DNA conformation : The respective single HMG box in HMG2 protein has DNA-binding activity. The tandem array of the two HMG boxes strengthens the activity. One of the major factor of the stronger DNA-binding activity of box B is resulted from the intercalation of side chain of F107. Computer simulation for HMG2 protein structure showed a structural model in which two boxes are linked in head-to-head manner.
The major functional region in HMG2 protein for its preferential binding with supercoiled DNA, DNA unwinding activity, and DNA protection activity from nuclease S1 digestion is in box B flanked by the neighboring sequences.
(2) The functions of HMG1/2 proteins : The functional analyses for HMG proteins 1 and 2 revealed that these proteins have multiple functions concerned in transcription, DNA replication, DNA recombination, DNA repair and DNA integration of outer DNA into chromosomal DNA.
|
-
-
-
-
-
-
-
-
-
-
[Publications] Yamamoto, A.Ando, Y., Yoshioka, K., Saito, K., Tanabe, T., Shirakawa, H.and Yoshida, M.: "Difference in affinity for DNA between HMG proteins 1 and 2 detemined by surface plasmon resonance measurements." J.Biochem.122. 586-594 (1997)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Shirakawa, H., Tanigawa, T., Kobayashi, M., Terashima, T., Yoshida, K., Arai, T.and Yoshida, M.: "Nuslear accumulation of HMG2 protein is mediated by basic regions interspaced with a long DNA-binding sequence, and retention within the nucleus requires the acidic carboxyl terminus." Biochemistry. 36. 5992-5999 (1997)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Sobajima, J., Ozaki, S., Osakada, F., Uesugi, H., Shirakawa, H., Yoshida, M.and Nakao, K.: "Novel autoantigens of pANCA in elcerative colitis : non-histone chromosomal proteins, HMG1 and HMG2." Clin.Exp.Immunol.107. 135-140 (1997)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Yumoto, Y., Shirakawa, H., Yoshida, M., Suwa, A., Watanabe, F., and Teraoka, H.: "High mobility group proteins 1 and 2 can function as DNA-binding regulatory components for DNA-dependent protein kinase in vitro." J.Biochem.124. 519-527 (1998)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Nagaki, S., Yamamoto, M., Yumoto, y., Shirakawa, H., Yoshida, M.and Teraoka, H.: "Non-histone chromosomal proteins HMG1 and 2 enhance ligation reaction of DNA double-strand breaks." Biochem.Biophys.Res.Commun.246. 137-141 (1998)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Sobajima, J., Ozaki, S., Uesugi, H., Osakada, F., Shirakawa, H., Yoshida, M.and Nakao, K.: "Prevalence and characterization of perinuclear anti-neutrophil cytoplasmic antibodies (P-ANCA) directed against HMG1 and HMG2 in ulcerative colitis (UC)." Clin.Exp.Immunol.111. 402-407 (1998)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Yoshioka, K., Saito, K., Tanabe, T., Yamamoto, A., Ando, Y., Nakamura, Y., Shirakawa, H.and Yoshida, M.: "Differences in DNA recognition and conformational change activity between boxes A and B in HMG2 protein." Biochemistry. 38. 589-595 (1999)
Description
「研究成果報告書概要(欧文)」より
-
-
