Analysis of the mechanism by nobel regulators of endothelin receptor signaling

1998 Fiscal Year Final Research Report Summary

• Project/Area Number: 09680613
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Chiba University Graduate School of Medicine
• Principal Investigator: KIMURA Sadao Chiba University Graduate School of Medicine, Department of Biochemistry and Molecular Pharmacology, Proffesor, 大学院・医学研究科, 教授 (40134225)
• Co-Investigator(Kenkyū-buntansha): MOROI Kayoko Chiba University Graduate School of Medicine, Department of Biochemistry and Mol, 大学院・医学研究科, 助手 (80110352) ; NISHIYAMA Mariko Chiba University Graduate School of Medicine, Department of Biochemistry and Mol, 大学院・医学研究科, 助手 (00092081)
• Project Period (FY): 1997 – 1998
• Keywords: Endothelin / Endothelin receptors / Endothelin receptor antagonists / Desensitization / Binding experiment / Regulators of G protein signaling

Research Abstract

(1) [^<25>I]endothelin(ET)-l binding and functional properties of CHO cells stably coexpressing ETA and ETB receptors were studied. The results provide a new insight into relationship between ET receptors and their ligands, demonstrating that ET-1 displaced from ETB receptors by ETB receptor antagonists is trapped by ETA receptors and causes ETA receptor-mediated responses. (2) RGS (regulator of G protein signaling) genes which present in heart and smooth muscle were transiently expressed into cells and their suppression activities of ET-1-mediated responses were evidenced.

Research Products

• [Publications] Tanaka, H., Moroi, K., Nishiyama, M., Kimura, S.et al.: "Novel mutations of the endothelin B receptor gene in patients with Hirschsprung disease and their characterization." J.Biol.Chem.273. 11378-11383 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shibasaki, T., Moroi, K., Nishiyama, M., Kimura, S.et al.: "Characterization of the carboxyl terminal-truncated endothelin B receptor coexpressed with G protein-coupled receptor kinase 2." Biochem.Mol.Biol.Int.(in press). (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 木村 定雄: "エンドセリン研究の最前線" Progress in Medicine. 18. 2073-2081 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] H.Tanaka, K.Moroi, J.Iwai, H.Takahashi, N.Ohnuma, S.Hori, M.Takimoto, M.Nishiyama, T.Masaki, M.Yanagisawa, S.Sekiya, S.Kimura: "Novel mutations of the endothelin B receptor gene in patients with Hischsprung's disease and their characterization." J.Biol.Chem.273. 11378-11383 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] T.Shibasaki, K.Moroi, M.Nishiyama, J.Zhou, A.Sakamoto, T.Masaki, K.Itoh, T.Haga, S.Kimura.: "Characterization of the carboxyl terminal-truncated endothelin B receptor coexpressed with G protein-coupled receptor kinase 2." Biochem.Mol.Biol.Int.(in press). (1999)
  • Description: 「研究成果報告書概要(欧文)」より