1998 Fiscal Year Final Research Report Summary
Functional Analysis of chondroitin sulfate by knockout technique
| Project/Area Number |
09680616
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
MURAMATSU Hisako School of Medicine, Nagoya University Assistant Professor, 医学部, 助手 (50182134)
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| Co-Investigator(Kenkyū-buntansha) |
HABUSHI Osami Aichi University of Education, Faculty of Education, Professor, 教育学部, 教授 (90024067)
MURAMATSU Takashi School of Medicine, Professor, 医学部, 教授 (00030891)
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| Project Period (FY) |
1997 – 1998
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| Keywords | chondroitin 6-sulfate / sulfotransferase / gene knockout / N-acetylglucosamine |
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| Research Abstract |
Genomic DNA of mouse chondroitin 6-sulfotransferase was used to construct targeting vector to delete the gene. Knockout mice devoid of chondroitin 6-sulfotransferase gene were born and could reproduce. Enzymatic assay confirmed the absence of the sulfotransferase activity in the knockout mice. Therefore, we concluded that chondroitin 6 sulfate is not necessary for development and reproduction. Possible abnormality of the knockout mice in immunological activities is under continued investigation. We found a mouse cDNA which has homology to chondroltin 6-sultotransferase and revealed that the new cDNA encoded elusive N-acetylglucosamine 6-sulfotransferase. The enzyme was involved in synthesis of 6-sulfo sialyl Lewis X structure, and was expressed in high endothelial venules of lymph nodes. Cotransfection of the cDNA and that of fucosyltransferase VII were able reconstitute functional L-selectin ligand on endothelial cells. To find out general function of 6-sulfo N-acetylglucosamine-containing structures, we are on the way to knockout the N-acetylglucosamine 6-sulfotransferase gene, again by isolating the gene and construction of the targeting vector.
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[Publications] Uchimura, K., Kadomatsu, K., Fan, Q.-W., Muramatsu, H., Kurosawa, N., Kaname, T., Yamamura, K., Fukuta, M., Habichi, O and Muramatsu, T.: "Mouse chondroitin 6-sulfotransferase : Molecular cloning, characterization and chromosomal mapping" Glycobiology. 5. 489-496 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Igakura, T., Kadomatsu, K., Kaname, T., Muramatsu, H., Fan, W.-W., Miyauchi, T., Takahashi, M., Senda, M., Taguchi, O., Kuno, N., Yamamura, K., Arimura, K.and Muramatsu, T.: "Basigin, a member of the immunoglobulin superfamily, plays important roles in periimplantation embryogenesis and spermatogenesis" Dev.Biol.194. 152-165 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Uchimura, K., Muramatsu, H., Kadomatsu, K., Fan, Q.-W., Kurosawa, N., Mitsuoka, C., Kannagi, R., Habuchi, O.and Muramatsu, T.: "Molecular cloning and characterization of an N-acetylglucosamine 6-O-sulfotransferase" J.Biol.Chem.273. 22577-22583 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Uchimura, K., Muramatsu, H., Kaname, T., Ogawa, H., Yamakawa.T., Fan, Q.-W., Mitsuoka, C., Kannagi, R., Habuchi, O., Yokoyama, I., Yamamura, K., Ozaki, T., Nakagawara, A., Kadomatsu, K.and Muramatsu, T.: "Human N-acetylglucosamine-6-O-sulfotransferase involved in the biosynthesis of 6-sulfo sialyl Lewis X : Molecular cloning, chromosomal mapping, and expression in various organs and tumor cells" J.Biochem.124. 670-678 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Nakamura, E., Kadomatsu, K., Yuasa, S., Muramatsu, H., Mamiya, T., Nabeshima, T., Fan, Q.-W., Ishiguro, K., Igakura, T., Matsubara, S., Kaname, T., Horiba, M., Saito, H.and Muramatsu, T.: "Disruption of the midkine gene (Mdk) resulted in altered expression of a calcium binding protein in the hippocampus of infant mice and their abnormal behavior" Genes to Cells. 3. 811-822 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Fan, Q.-W., Uchimura, K., Yuzawa, Y., Matsuo, S., Mitsuoka, C., Kannagi, R., Muramatsu, H., Kadomatsu, K.and Muramatsu, T.: "Spatially and temporally regulated expression of N-acetylglucosamine-6-O-sulfotransferase during mouse embryogenesis" Glycobiology. (in press).
Description
「研究成果報告書概要(欧文)」より
