1998 Fiscal Year Final Research Report Summary
Regulation of the Na+/H+ exchanger and signal transduction
| Project/Area Number |
09680642
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
WAKABAYASHI Shigeo Dept.Mol.Physiol.NCVC, 循環分子生理部, 室長 (70158583)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Yoko Dept.Mol.Physiol.NCVC, 循環分子生理部, 流動研究員
IKEDA Toshitaro Dept.Mol.Physiol.NCVC, 循環分子生理部, レジデント
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| Project Period (FY) |
1997 – 1998
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| Keywords | Na+ / H+ exchanger / volume regulation / calcineurin homologue protein / calmodulin |
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| Research Abstract |
The Na/He exchanger (NHE) is an important regulator for intracellular pH (pH), C* volume, and transepithelial Na^+ transport. It exists in virtually all cells with cell ty* dependent pattern of isoform expression. Among six known isoforms of NHE, a ubiquito* isoform NHE1 is known to be rapidly activated in response to a variety of extracellul* stimuli ranging from growth factors to mechanical stimuli such as hyperosmotic stress a* cell spreading. Some accessory regulatory factors has been suggested to be involved in * regulation of NHE1. Galmodulin is the first identified regulatory factor that is involved Ca^<2+>-induced activation of NHE1. Recently, the calcineurin B homologous protein (CHP) h* been cloned as a candidate for such regulatory factors of NHE1. In recent two years, w* tried to identify CHP binding domain within NHE1. A cytoplasmic region of aa 515-530 w* identified as a strong CHP-binding domain. However, we have not yet determine t* precise role of CHP in the regulation of NHE1. We also tried to identify an important regi* of NHE for volume regulation and found that the first extracellular loop is a critical region f* positive or negative response to volume change in NHE isoforms.
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[Publications] Wakabayashi, S., Ikeda, T., Kobayashi, Y., Iwamoto, T., and Shigekawa, M.: "The Na+/H+ exchanger and volume regulation" In“Cell volume regulation : The moledular mechanism and volume sensing mashinery", edited. by Y. Okada, Elsevier Publishers.167-171 (1998)
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[Publications] Wakabayashi, S., Ikeda, T., Kobayashih, Y., Iwamoto, T., and Shigekawa, M.: "The Na^+/H^+ exchanger and volume regulation." In"Cell volume regulation : The molecular mechanism and volume sensing machinery", edited.by Y.Okada, Elsevier Publishers.167-171 (1998)
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[Publications] Ikeda, T., Schmitt, B., Pouyssegur, J., Wakabayashi, S., and Shigekawa, M.: "Identification of cytoplasmic subdomains that control pH-sensing of the Na^+/H exchanger (NHE1). pH-Maintenance, ATP-sensitive, and flexible loop domains" J.Biochem.(Tokyo). 121. 295-303 (1997)
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「研究成果報告書概要(欧文)」より
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