1998 Fiscal Year Final Research Report Summary
Function of transcription factors mafs in the cellular differentiation
| Project/Area Number |
09680665
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
SAKAI Masaharu School of Medicine, Hokkaido University Associate Professor, 医学部, 助教授 (50162269)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Transcription factor / Oncogene / Differentiation / maf / Chondrocyte / Lens / Spinal code / Kidney |
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| Research Abstract |
maf is a family of oncogenes originally identified from avian oncogenic retrovirus, AS42, encoding a nuclear bZip transcription factor. We have isolated two maf related cDNA clones, maf-l(maf-B) and maf-2(c-maf), from a rat liver cDNA library. Both genes are expressed at low levels in a wide variety of rat tissue, including spleen, kidney, muscle and liver. Immunohistochemical studies and in situ hybridization analyses show that maf-1 and maf-2 are strongly expressed in the late stages of chondrocytes development in the femur epiphysis and the rib and limb cartilage of l5day old (E15) embryo in rat. In situ hybridization analyses of E15 embryos show both genes are expressed in the eye lens and the spinal cord as well as the cartilage. However, the expression patterns of maf-1 and maf-2 in lens and spinal cord were different. mafs are also expressed in developing kidney, maf-1 expressed in glomerular visceral epithelial and maf-2 expressed in renal tubules, and both expressions were never overlapped, In the differentiation process of the adipocytes, maf-2 expression was dramatically down regulated in differentiation specific manner. These results suggest that both transcription factors have closely related but different functions.
Determination of specificity of the heterodimaer formation indicates that both factors significantly different properties and they might be have different target genes. Heterodimer formations of these factors dramatically change the specificity of DNA binding and transcriptional activity from that of homodimer. These findings suggest that cellular concentration and affinities of dimer formation of these factors might be very important for transcriptional modulation.
The genomic clones of maf-l and maf-2 genes were isolated and the analyses of regulation of these genes show that auto regulation mechanism is worked on both genes.
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Research Products
(14 results)
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[Publications] Sakai, M., Imaki, J., Yoshida, K., Ogata, A., Matsushima-Hibiya, Y., Kuboki, Y., Nishizawa, M.and Nishi, S.: "Rat maf related gene : Specific expression in chondrocytes, lens and spinal cord" Oncogene. 14. 745-750 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Watanabe, M., Miura, Y., Ido, A., Sakai, M., Nishi, S., Inoue, Y., Hashimoto, T., and Tamaoki, T.: "Developmental changes in expression of the ATBF1 transcription factor gene" Mol.Brain Res. 42. 344-349 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hirokawa, J., Sakaue, S., Tagami, S., Kawakami, Y., Sakai, M., Nishi, S., and Nishihira, J.: "Identification of macrophage migration inhibitory factor in adipose tissue and its induction by tumor necrosis factor-alpha." Biochem.Biophys.Res.Commun.235. 94-98 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Yoshida, K., Imaki, J., Koyama, Y., Harada, T., Shinmei, Y., Oishi, C., Matsushima-Hibiya, Y., Matsuda, A., Nishi, S., Matsuda, H.and Sakai, M.: "Differential expression of maf-1 and maf-2 genes in the developing rat lens" Inves.Ophthal.Visu.Sci.38. 2679-2683 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Nishihira, J., Fujunaga, M., Kuriyama, T., Suzuki, M., Sugimoto, H., Nakagawa, A., Tanaka, I., and Sakai, M.: "Molecular cloning of Hman D-Dopachrome Tautomerase cDNA : N-Terminal Proline is Essential for Enzyme activation." Biochem.Biophys.Res.Commun.243. 538-544 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Hirokawa, J., Sakaue, S., Furuya, Y., Ishii, J., Hasegawa, A., Tagami, S., Kawakami, Y., Sakai, M., Nishi, S.and Nishihira, J.: "Tumor necrosis factor-alpha regulates the gene expression of macrophage migration inhibitory factor through tyrosine kinase-dependent pathway in 3T3-L1 adipocytes" J.Biochem.123. 733-739 (1998)
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「研究成果報告書概要(欧文)」より
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