• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1998 Fiscal Year Final Research Report Summary

REGULATION OF DIFFERENTIATION BY BACH TRANSCRIPTION FACTORS

Research Project

Project/Area Number 09680667
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionTohoku University (1998)
University of Tsukuba (1997)

Principal Investigator

IGARASHI Kazuhiko  TOHOKU UNIV.SCHOOL OF MEDICINE,ASSOCIATE PROFESSOR, 医学部, 助教授 (00250738)

Project Period (FY) 1997 – 1998
Keywordsoncogene / AP-1 / Maf / Bach
Research Abstract

To understand molecular regulation of cell differentiation, it is essential to reveal regulation of transcription in these processes. Bachi and Bach2 are closely related transcription factors that bind to DNA by forming heterodimers with Maf family of transcription factors. During the past two years supported by this grant, we accumulated results that suggested that Bachi and Bach2 are involved in regulation of hematopoiesis, and that they function as a novel type of architectural transcription fadors.
(1) The BTB domain of Bach1 and Bach2 appears to be involved in regulation of chromatin structure. Using atomic force microscopy, we revealed that Bach1, and possible Bach2, fundion as a novel type of architectural transcription factor that mediate DNA loop formation. As such, Bach1 may be involved in regulation of the globin gene locus control region.
(2) Expression of Bach2 is specific to B cells among hematopoietic cells. Its expression is high during early stages and down regulated upon maturation to plasmacytes. Bach2 represses 3' enhancer of the immunoglobulin heavy chain gene. Thus Bach2 appears to regulate stage-specific gene expression during B cell differentiation.
(3) Using yeast two hybrid screen, we isolated a novel transcription factor, FOB (friend of Bach), as aprotein that binds to the BTB domain of Bach. FOB carries the BTB domain as well as a C2H2-typeZn fingers. Expression profile of FOB suggested that it is a partner of Bach1 and/or Bach2 inhematopoietic cells.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Nagai, T.: "Regulation of NF-E2 activity in erythroleukemia cell differentiation." Joumal of Biological Chemistry. 273. 5358-5365 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Igarashi, K.: "Multivalent DNA Binding Complex Generated by Small Maf and Bach1 as a Possible Biochemical Basis for β-globin Locus Control Region Complex." Journal of Biological Chemistry. 273. 11783-11790 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Muto, A.: "ldentification of Bach2 as a B cell-specific partner for small Maf proteins that negatively regulate the immunoglobulin heavy chain gene3' enhancer" EMBO Journal. 17. 5734-574〓 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Itoh, K.: "Keapl represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain." Genes and Development. 13. 76-86 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi, A.: "Molecular cloning and functional characterization of a new Cap‘n'collar family transcription factor Nrf3." Joumal of Biological Chemistry. 274. 6443-6452 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagai, T., Igarashi, K., Akasaka, J., Furuyama, K., Fujita, H., Hayashi, N., Yamamoto, M.and Sassa, S.: "Regulation of NF-E2 activity in erythroleukemia cell differentiation" J.Biol.Chem.273. 5358-5365 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Igarashi, K., Hoshino, H., Muto, A., Suwabe, N., Nishikawa, S., Nakauchi, H., and Yamamoto, M.: "Multivalent DNA Binding Complex Generated by Small Maf and Bach1 as a Possible Biochemical Basis for beta-globin Locus Control Region Complex." J.Biol.Chem.273. 11783-11790 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Muto, A., Hoshino, H., Madisen, L., Yanai, N., Obinata, M., Karasuyama, H., Hayashi, N., Nakauchi, H., Yamamoto, M., Groudine, M., and Igarashi, K.: "Identification of Bach2 as a B cell-specific partner for small Maf proteins that negatively regulate the immunoglobulin heavy chain gene 3' enhancer" EMBO. 17. 5734-5743 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ItoH,K., Wakabayashi, N., Katoh, Y., Ishii, T., Igarashi, K., Engel, J.D., and Yamamoto, M.: "Keap1 represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain." Genes Dev.13. 76-86 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi, A., Ito, E., Toki, T., Kogame, K., Takahashi, S., Igarashi, K., Hayashi, N., and Yamamoto, M.: "Molecular cloning and functional characterization of a new Cap'n'collar family transcription factor Nrf3." J.Biol.Chem.274. 6443-6452 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi