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1998 Fiscal Year Final Research Report Summary

Function and molecular properties of nuclear pore-targeting complex

Research Project

Project/Area Number 09680692
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionOsaka University

Principal Investigator

IMAMOTO Naoko  Osaka University Medical School, Assistant Professor, 医学部, 助手 (20202145)

Project Period (FY) 1997 – 1998
Keywordsnuclear protein transport / Importin α / Importin β / nuclear pore-targeting complex / nuclear localization signal / Ran / β-catenin
Research Abstract

Nuclear import of proteins arc initiated by nuclear localization signal (NLS)-dependent complex formation, termed the nuclear pore-targeting complex (PTAC), in the cytoplasm. PTAC is a stable protein complex composed of a karyophilic protein and two cytosolic components termed PTAC58/importin α and PTAC97/importin β. Importin α directly binds to NLS of karyophile and importin β provides nuclear pore complex (NPC) binding site for PTAC. We analyzed the properties of importin α and importin β, and obtained the following results : 1. We have identified three additional mouse genes homologous to the known importin α, and showed that the mouse importin α can be classified into three subfamilies (Rch1, NPI1, and Qip1), showing approximately 50% amino acid identity to one another. 2. We showed that importin α family proteins show some specificities in the recognition of substrates. For example, Qip1 recognizes a very restricted NLS, requiring both the upstream sequence and basic core sequence … More of Q1 ATPase for its substrate recognition. The other evidence is that tyrosine phosphorylated Stat his recognized by NPI1, but not Rch1. Stat 1 apparently does not possess typical basic-type NLS, and it binds to different portion of NPI1 from those of SV40 T-antigen basic NLS binding site. 3. We examined the behavior and functional domain of importin β Importin β rapidly shuttles between the cytoplasm and the nucleus through NPCs. The use of deletion mutants reveals that NPC-translocation of importin β requires neither Ran- nor importin α-binding but only the NPC-binding domain of this molecule. NPC translocation of importin β was found to require neither Ran nor its GTP hydrolysis. Furthermore, importin β possesses ability to translocate NPC on its own. This could be a common feature of transport factors that carries certain cargos through NPCs into and out of the nucleus. 4. We examined the nuclear import of β-catenin and found that b-catenin can translocate NPCs on it own without requiring soluble factors including Ran. Β-catenin is likely to share the same property with importin β for NPC translocation. Less

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Fumihiko Yokoya: "β-Catenin can be transported into the nucleus in Ran-unassisted manner."Mol.Biol.Cell. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miki Hieda: "Monoclonal antibody to the COOH-terminal acidic portion of Ran Inhibits both the recycling of Ran and nuclear protein import in living cells."J.Cell.Biol.. 144. 645-655 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shingo Kose: "β-Subunit of nuclear pore-targeting complex (Importin -β) can be exported from the nucleus in a Ran-independent manner."J.Biol.Chem.. 274. 3946-3952 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masami Yamada: "Nuclear transport factor p10/NTF2 functions as a Ran-GDP dissociation inhibitor (Ran-GDI)."Curr.Biol.. 8. 1339-1342 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naoko Imamoto: "Nuclear transport factor : Function,behavior and interaction"Eur.J.Histochem. 42. 9-20 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahiro Matsusaka: "Mutations in fission yeast Cut15,an importin α homolog,lead to mitotic progression without chromosome condensation."Curr.Biol.. 8. 1031-1034 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chang-Jie Jiang: "Functional characterization of a plant importin α homologue : Nuclear localization signal (NLS)-selective binding and mediation of nuclear import of NLS-proteins in vitro."J.Biol.Chem.. 273. 2408-2408 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chang-Jie Jiang: "In vitro characterization of rice importin β-1 : Molecular interaction with nuclear transport factors and mediation of nuclear protein import."FEBS Lett.. 437. 127-130 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shingo Kose: "Ran-unassisted nuclear migration of a 97KDa component of nuclear pore-targeting complex"J.Cell.Biol.,. 139. 841-849 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiro Sekimoto: "Extracellular signal-dependent nuclear import of Statl is mediated by nuclear pore-tageting complex formation with NPI-1,but not Rchl"EMBO J.,. 16. 7067-7077 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoichi Miyamoto: "Differential modes of nuclear localization signal (NLS) recognition by three distinct classes of NLS receptors"J.Biol.Chem.,. 272. 26375-26381 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lyuji Tsuji: "Identification of novel homologues of mouse importimα,the a subunit of the nuclear pore-targeting complex,and their tissue specific expressiom"FEBS Lett.,. 416. 30-34 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noriko Yasuhara: "Essential roles of active nuclear trasport in apoptosis"Genes to Cells. 2. 55-64 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naoko Imamoto: "Membrane protein insertion and Translocation"Portland Press,UK,(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 横屋史彦・今本尚子: "細胞工学"秀潤社,(4月号 予定). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fumihiko Yokoya: "β-Catenin can be transported into the nucleus in Ran-unassisted manner"Mol. Biol. Cell. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miki Hieda: "Monoclonal antibody to the COOH-terminal acidic portion of Ran Inhibits both the recycling of Ran and nuclear protein import in living cells"J. Mol. Cell. 144. 645-655 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shingo Kose: "β-Subunit of nuclear pore-targeting complex (Importin-β) can be exported from the nucleus in a Ran-independent manner"J. Biol. Chem.. 274. 3946-3952 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masami Yamada: "Nuclear transport factor p10/NTF2 functions as a Ran-GDP dissociation inhibitor (Ran-GDI)"Curr. Biol.. 8. 1339-1342 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naoko Imamoto: "Nuclear transport factor Function, behavior and interaction"Eur J. Histochem. 42. 9-20 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahiro Matsusaka: "Mutations in fission yeast Cut 15, an importin α homolog, lead to mitotic progression without chromosome condensation"Curr. Biol.. 8. 1031-1034 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chang-Jie Jiang: "Functional characterization of a plant importin α homologue : Nuclear localization signal (NLS)-selective binding and mediation of nuclear import of NLS-proteins in vitro"J. Biol. Chem.. 273. 24083-24087 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chang-Jie Jiang: "In vitro characterization of rice importin β-1 : Molecular interaction with nuclear transport factors and mediation of nuclear protein import"FEBS Lett.. 437. 127-130 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shingo Kose: "Ran-unassisted nuclear migration of a 97kDa component of nuclear pore-targeting complex"J. Cell Biol.. 139. 841-849 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshihiro Sekimoto: "Extracellular signal-dependent nuclear import of Stat1 is mediated by nuclear poretargeting complex formation with NPI-1, but not Rch1"EMBO J.. 16. 7067-7077 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoichi Miyamoto: "Differential modes of nuclear localization signal (NLS) recognition by three distinct classes of NLS receptors"J. Biol. Chem.. 272. 26375-26381 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lyuji Tsuji: "Identification of novel homologne of mouse importing, the a subunit of the nuclear poretargeting complex, and their tissue specific expression"FEBS Lett.. 416. 30-34 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriko Yasuhara: "Essential roles of active nuclear transport in apoptosis"Gene to Cells. 2. 55-64 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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