1999 Fiscal Year Final Research Report Summary
Study on the topology of the GPI anchor assembly
| Project/Area Number |
09680707
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Fukuoka University |
|---|
Principal Investigator |
HIROSE Shinichi Fukuoka Univ., Sch. of Med., Assoc. Prof., 医学部, 助教授 (60248515)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MISUMI Yoshio Fukuoka Univ., Sch. of Med., Assoc. Prof., 医学部, 助教授 (10148877)
IKEHARA Yukio Fukuoka Univ., Sch. of Med., Professor, 医学部, 教授 (70037612)
|
|---|
| Project Period (FY) |
1997 – 1999
|
| Keywords | GPI anchor |
|---|
| Research Abstract |
The glycophosphatidylinositol (GPI) anchor is preassembled and transferred to the c-terminus of the corresponding nascent proteins in the ER. The assembly starts in the luminal side of the ER and proceeds between the cytosolic and luminal sides of the ER. Thus, precursors of GPI anchor have to flip-flop between the lipid bilayer during the assembly. The mechanisms underlying this flip-flop has not been well characterized, nor identified are cues for the flip-flop. The GPI anchor proteins on some cell lines are known to be acylated while most of the GPI proteins are not acylated. This implied that acylation involved in the flip-flop as a cue. We have investigated the timing of the flip-flop in the relation, with the acylation of the precursors on erythroleukemia cells. The GPI anchor including the precursors of which inositol residue is acylated are resistant GPI specific pshopholipase C digestion.
The deacylation took after the transfer of the preassembled GPI to the target nascent proteins. This findings thus suggested that the acylation is not involved in the flip-flop.
|
