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1998 Fiscal Year Final Research Report Summary

"MOLECULAR MODE OF ACTION OF NEUROTROPHIC FACTOR,PROSAPOSIN"

Research Project

Project/Area Number 09680764
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionEHIME UNIVERSITY

Principal Investigator

SANO Akira  EHIME UNIVERSITY, 医学部, 助教授 (30178800)

Co-Investigator(Kenkyū-buntansha) UENO Shu-ichi  EHIME UNIVERSITY, 医学部附属病院, 助手 (80232768)
Project Period (FY) 1997 – 1998
KeywordsPROSAPOSIN / NEUROTROPHIC / 18-MER PEPTIDE / DOPAMINERGIC / NEURON / ドーパミン / パーキンソン病 / ラット海馬神経細胞
Research Abstract

Prosaposin, the precursor of saposins which activate sphingolipid hydrolases, has been identified as a neurotrophic factor. The neurotrophic activity of prosaposin on human neuroblastoma cells, hippocampal neurons, and PC 12 cells has been shown to reside in the NH_2-terminal hydrophilic sequence of the C-domain of prosaposin. It has recently demonstrated that intracerebral transplantation of testis-derived sertoli cells promotes functional recovery in rats with 6-hydroxydopamine-induced hemiparkinsonism, and in vitro experiments demonstrated enhanced dopaminergic neuronal survival and outgrowth when embryonic neurons were cultured with medium in which rat Sertoli cells had been grown. Since prosaposin is one of the major Sertoli cell secretory proteins, its trophic effect on dopaminergic neurons is conceivable. Here we show that a peptide comprising the 18-amino-acid sequence (18-mer peptide ; LSELIINNATEELLIKGL) located in the NH_2-terminal hydrophilic sequence of the C-domain of rat prosaposin promoted survival and neurite outgrowth of tyrosine hydroxylase-positive neurons on embryonic day 15 rat ventral mesencephalic cultures.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Patton et al.: "Prosaposin a Neurotrophic Factor:Presence and Properties in Boune Milk" J.Pairy Sci.80. 264-272 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kushihata et al.: "Expression of basic fibroblast growth factor-like immunoreactiuty in the nuclei of regenerating hepafocytes." Cell.Tiss.Res.288. 517-527 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小谷泰教・佐野 輝: "プロサポシンの神経栄養因子作用" 医学のあゆみ 別冊 神経細胞死制御. 233-237 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐野 輝(分担): "領域別症候群シリーズNo19 先天性代謝異常症候群(下巻)" 日本臨床, (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Patton, G.S.Carson, M.Hiraiwa, J.S.O'Brien, and A.Sano: "Prosaposin a Neurotrophic Presence and Properties in Bovine Milk." J.Dairy Sci. 80. 264-272 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] F.Kushihata, S.Matsuda, A.Sano, J.Aburaya, Y.Shimahara, N.Kobayashi, and M.Sakanaka: "Expression of basic fibroblast growth factor-like immunoreactivity in the nuclei of regenerating" Cell Tiss.Res.288. 517-527 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Sano: "Saposin C deficiency." Ryoikibetus Shokogun Shirizu. 19 Pt 2. 240-2 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Kotani and A.Sano: "Saposin B deficiency." Ryoikibetus Shokogun Shirizu. 19 Pt 2. 417-9 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kondoh and A.Sano: "Prosaposin deficiency." Ryoikibetus Shokogun Shirizu. 19 Pt 2. 414-6 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kondoh and A.Sano: "GM2 activator protein deficiency." Ryoikibetsu Shokogun Shirizu. 19 Pt 2. 411-3 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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