ACTIVITY-DEPENDENT REGULATION OF NEURAL CELL ADHESION MOLECULES, NEUROTROPHINS AND THEIR RECEPTORS IN NEURONS.

1999 Fiscal Year Final Research Report Summary

• Project/Area Number: 09680783
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH
• Principal Investigator: ITOH Kouichi TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH, THE TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE, RESEARCHER, 東京都臨床医学総合研究所, 研究員 (30291149)
• Project Period (FY): 1997 – 1999
• Keywords: NEURONAL ACTIVITY / NEURAL CELL ADHESION MOLECULES / NEUROTROPHIC FACTORS / trk / DRG NEURONS / CELL CULTURES / MOUSE / ELECTRICAL STIMULATION

Research Abstract

We studied the relationship between neuronal activity, neural cell adhesion molecules and neurotrophins in mouse dorsal root ganglion (DRG) neurons during development in vitro. The nervous system structure is modified by electrical activity in developing neural circuits, but the molecular mechanisms are not well under stood. Recognition molecules and neurotrophic factors regulate cell interactions during development. Therefore, the possible involvement of neural cell adhesion molecules (L1, NCAM and N-cadherin), and of neurotrophins (NGF, BDNF and NT-3) in activity-development was studied by delivering electrical pulses to mouse DRG neurons in cultures. After 5 days exposure to NGF, but not after exposure to BDNF, the NT-3 and L1 levels increased 3〜4 fold. Low frequency stimulation (0.1 Hz for 5 days) decreased L1 and trkC levels in the absence of NGF. Regulation of the expression of L1 and trkC by specific patterns of impulse activity may influence cell interactions coordinating structure and function of the nervous system at critical developmental stages.

Research Products

• [Publications] K. Itoh, et al.: "Fields,Activity-dependent regulation of N-cadherin in DRG neurons : Differential regulation of N-cadherin, NCAM and L1 by distinct patterns of action potentials"J.Neurobiol.. 33. 735-748 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] R. D. Fields, et al.: "Action potential-dependent regulation of gene expression : Temporal specificity in Ca^<++>, CREB and MAP kinase signaling"J.Neurosci.. 17. 7252-7266 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Yoshimura, et al.: "A monoclonal antibody, 14F7, which recognizes a stage-specific immature oligodendrocyte surface molecule inhibits, oligodendrocyte differentiation mediated in co-culture with astrocytes"Neurosci. Res.. 54. 79-96 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] H.Asou, Y.Takeda, K.Itoh and K.Uyemura,: "The RSLE sequence in the cytoplasmic domain of adhesion molecule L1 is involved in cell migration on L1 substrate (K. Uyemura, K. Kawamura and T. Yazaki, edts)."Neural Development/Keio Univ Symp for Life Sci and Med. 267-272 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K.Itoh,: "Activity-dependent regulation of neural cell adhesion molecules and neurotrophins in neurons."Bull Cell Sci Res Fund. 10. 35-44 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K.Yoshimura, Y.Sakurai, D.Nishimura, Y.Tsuruo, M.Nomura, K.Kawato, C.Seiwa, T.Iguchi, K.Itoh and H.Asou,: "A monoclonal antibody, 14F7, which recognizes a stage-specific immature oligodendrocyte surface molecule inhibits, oligodendrocyte differentiation mediated in co-culture with astrocytes."J. Neurosci. Res.. 54. 79-96 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K.Itoh, M.N.Ozaki, B.Stevens and R.D.Fields,: "Activity-dependent regulation of N-cadherin in DRG neurons: Differential regulation of N-cadherin, NCAM and L1 by distinct patterns of action potentials."J. Neurobiol.. 33. 735-748 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] R.D.Fields, F.Eshete, B.Stevens and K.Itoh,: "Action potential-dependent regulation of gene expression: Temporal specificity in CaィイD1++ィエD1, CREB and MAP kinase signaling."J. Neurosci.. 17. 7252-7266 (1997)
  • Description: 「研究成果報告書概要(欧文)」より