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1998 Fiscal Year Final Research Report Summary

The physiological significance and the change of the neurosteroid-producing enzyme in the brain under stress conditions.

Research Project

Project/Area Number 09680794
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionWakayama Medical College (1998)
The University of Tokushima (1997)

Principal Investigator

TSURUO Yoshihiro  Wakayama Medical College, Medical School, Professor, 医学部, 教授 (90207449)

Co-Investigator(Kenkyū-buntansha) MORITA Kyoji  Tokushima University, Medical School, Associate professor, 医学部, 助教授 (50108883)
Project Period (FY) 1997 – 1998
Keywords5alpha-reductase / Neurosteroid / Stress / Rat / Brain / Immunohistochemistry
Research Abstract

Steroid 5alpha-reductase is the enzyme that converts a number of steroids with a C-4,5 double bond and C-3 ketone to 5alpha-reduced metabolites, and the type 1 of two isozymes is present in the brain. We investigated immunohistochemically the change of the enzyme expression and the cellular appearance of 5alpha-reductase-containing rat C6 glioma cells by culturing these cells in vitro in stress conditions, that is, the serum-free medium. The expression of 5alpha-reductase was located in the perinuclear region under the serum-supplemented medium, but distributed in the cytoplasmic region under the serum-free medium. The expression of both GFAP and CNPase was the same between the two culture conditions. The cellular form was spinde in the serum-supplemented medium, and became hypertrophic and multipolar in the serum-free medium. However, the expression of 5alpha-reductase mRNA was the same indifferent of the presence of serum. The immunohistochemical change of 5alpha-reductase expression was induced by the change of enzyme localization within the cells, but not by the enzyme quantity.
In the adrenal gland, which is thought to be involved in the stress reactions, 5alpha-reductase was located in the fascicular to reticular layer of the gland and the staining intensity of the enzyme increased after the removal of gonads and decreased by the supplementation of testosterone or estradiol. The enzyme was shown to be located by light and electron microsopy in the cytoplasm of Schwann cells and satellite cells in the peripheral nervous system.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Tamura M: "Localization of NADPH diaphorase and vasoactive intestinal polypeptide-containing neurons in the efferent pathway to the rat corpus cavernosum" Eur. Urol.32. 100-104 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakurai Y: "Differentiation of oligodendrocyte occurs in contact with astrocyte" J.Neurosci. Res.52. 17-26 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshimura K: "Monoclonal antibody 14F7, which recognizes a stage-specific immature oligodendrocyte surface molecule, inhibits oligodendrocyte differentiation mediated in co-culture with astrocyte" J. Neurosci. Res.54. 79-96 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuruo Y: "Influence of serum-free culture conditions on subcellular localization of steroid 5d-reductase in rat C_6 glioma cells" Brain Res.801. 130-136 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoi H: "Leydig cells undergoing apoptosis in the perinatal rat testis" Acta Histochem.Cytochem.31. 355-361 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoi H: "Light and electron microscopic immunocytochemistry on the localization of 17d-hydroxylase/C17, 20-lyase (P450_<C17>) in the rat placenta" J.Med.Invest.44. 155-162 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoi H: "Steroid 5d-reductase type1 immunolocalized in the adrenal gland of normal, gonudectomized, and sex hormone-supplemented rats" Histochem, Cell Biol.109. 127-134 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoi H: "Steroid 5d-reductase type1 immunolocalized in the rat peripheral nervous system and paraganglia" Histochem. J.30. 731-739 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morita K: "Dexamethasone enhances serum deprivation-induced necrotic death of rat C6 glioma cells through uctivation of glucocorticoid receptors" Brain Res.816. 309-316 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morita K: "Influence of serum-free culture conditions on steroid 5d-reductase mRNA expression in rat C6 glioma cells" Brain Res.発表予定. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoi H: "Steroid 5d-reductase in supporting cells and Schwann cells of the rat adrenal medulla : light and electron microscopic study" Anat.Rec.発表予定. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamura, M., Kagawa, S., Tsuruo, Y., Ishimura, K., Kimura, K.and Kawanishi, Y.: "Localization of NADPH diaphorase and vasoactive intestinal polypeptide-containing neurons in the efferent pathway to the rat corpus cavernosum." Eur.Urol.32. 100-104 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakurai, Y., Nishimura, D., Yoshimura, K., Tsruo, Y., Seiwa, C.and Asou, H.: "Differentiation of oligodendrocyte occurs in contact with astrocyte." J.Neurosci.Res.52. 17-26 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshimura, K., Sakurai, Y., Nishimura, D., Tsuruno, Y., Nomura, M., Kawato, S., Seiwa, C., Iguchi, T., Itoh, K.and Asou, H.: "Monoclonal antibody 14F7, which recognizes a stage-specific immature oligodendrocyte surface molecule, inhibits oligodendrocyte differentiation mediated in co-culture with astrocytes." J.Neurosci.Res.54. 79-96 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuruo, Y., Ishimura, K.and Morita, K.: "Influence of serum-free culture conditions on subcellular localization of steroid 5alpha-reductase in rat C_6 glioma cells." Brain Res.801. 130-136 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoi, H., Yamama, Y., Turuo, Y., Kawarada, Y., Miura, N., Sugiyama, T.and Ishimura, K.: "Leydig cells undergoing apoptosis in the perinatal rat testis." Acta Histochem.Cytochem.31. 355-361 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoi, H., Tsuruo, Y., Nihira, M.and Ishimura, K.: "Light and electron microscopic immunocytochemistry on the localization of 17alpha-hydroxylase/C 17, 20-lyase (P450c17) in the rat placenta." J.Med.Invest.44. 155-162 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoi, H., Tsuruo, Y., Miyamoto, T.and Ishimura, K.: "Steroid 5alpha-reductase type 1 immunolocalized in the adrenal gland of normal, gonadectomized, and sex hormone-supplemented rats." Histochem.Cell Biol.109. 127-134 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoi, H., Tsuruo, Y., and Ishimura, K.: "Steroid 5alpha-reductase type 1 immunolocalized in the rat peripheral nervous system and paraganglia." Histochem.J.30. 731-739 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morita, K., Ishimura, K., Tsuruo, Y.and Wong, D.L.: "Dexamethasone enhances serum deprivation-induced necrotic death of rat C6 glioma cells through activation of glucocorticoid receptors." Brain Res.816. 309-316 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morita, K., Tsuruo, Y., Ishimura, K., Her, S., Rose, A.B.and Wong, D.L.: "Influence of serum-free culture conditions on steroid 5alpha-reductase mRNA expression in rat C6 glioma cells." Brain Res. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoi, H., Tsuruo. Y.and Ishimura, K.: "Steroid 5alpha-reductase in supporting cells of the rat adrenal medulla : light and electron microscopic study." Anat.Rec.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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