1998 Fiscal Year Final Research Report Summary
Feedback and feedforward neural control of muscle glucose uptake during contraction
| Project/Area Number |
09680839
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
NAKAMURA Takao Res.Inst.for Electro.Sci., Hokkaido Univ., Assoc.Prof., 電子科学研究所, 助教授 (00142654)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAUCHI Yoshiko Res.Inst.for Electro.Sci., Hokkaido Univ., Assist.Prof., 電子科学研究所, 助手 (50230313)
HARA Tsutomu Sch.of Med., Aichi Medical Univ., Lect., 医学部, 講師 (00198889)
KUSUNOKI Masataka Sch.of Med., Aichi Medical Univ., Lect., 医学部, 講師 (80214956)
KAWAHARA Koichi Res.Inst.for Electro.Sci., Hokkaido Univ., Prof., 電子科学研究所, 教授 (20125397)
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| Project Period (FY) |
1997 – 1998
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| Keywords | feedback control / feedforward control / the central nervous system / muscle contraction / muscle glucose uptake / muscle sympathetic nerve / microneurogram |
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| Research Abstract |
To clarify a possibility of muscle sympathetic (MS) nervous system (MSNS) as an efferent path of neurally controlled muscle glucose uptake (MGU) system during contraction, microneurographic technique has been tested in the sciatic nerve of anesthetized rats. After surgical methodology has been established and the location of MS fibers in the nerve bundle has been clarified, in the supine position, multi-fiber MSNA and blood glucose level (BG) were measured at rest as well as during induced muscle contraction in the ipsilateral hindlimb. To our best knowledge, this was the first case that the microneurography was used for the measurement of MS nerve activity (MSNA) in small animals. The data was stored in a data recorder, and analyzed off-line with a personal computer system. A criteria to identify MSNA have been established, MSNA was evaluated with conventional methods including the integrated neurogram, and a novel method using continuous wavelet transform has been developed for detecting action potentials. Results showed that the BG decreased during muscle contraction and that it was accompanied with an enhancement of MSNA, suggesting the MSNS is the efferent path in the neural feedback control system of MGU.
To approach the central feedforward control system, rat heads were fixed in a stereotactic frame, and methodology has been established to record MSNA in the prone position by microneurography. As a candidate of the control center in the central nervous system, neural signal in the ventromedial hypothalamus nuclei (VMH) has been tried to find out with a tungsten microelectrode, because VMH is believed to affect MGU at rest. However, it has not been established well yet to identify the location of or signal from VMH, It is considered to be necessary to develop new methods, including Fos method for example, to identify them in the future projects.
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[Publications] T.Hara, M.Kusunoki, K.Tsutsumi, K.Okada, S.Sakamoto, M.Ohnaka, T.Nakamura, T.Miyata, K.Nakayama, A.Fukatsu, K.Kato, S.Kakumu and Y.Nakaya: "A lipoprotein lipase activator, NO-1886, improves endothelium-dependent relaxation of rat aorta associated with aging" Eur.J.Pharmacol. 350. 75-79 (1998)
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