1998 Fiscal Year Annual Research Report

病態局所での好中球の活性化機構とシグナル伝達

Research Project

Project/Area Number	09770104
Research Institution	Osaka City University
Principal Investigator	佐藤英介大阪市立大学, 医学部, 助手 (60211942)
Keywords	好中球 / 活性酸素 / プライミング / 口腔内 / NF-kB
Research Abstract	循環血液中の好中球の活性酸素産生能は低いが、起炎物質などでプライミングされると数十倍に活性化される。これまでに、腫瘍壊死因子(TNF-α)や顆粒球コロニー刺激因子(G-CSF)等によるプライミングにはチロシンキナーゼの活性化と115kDa蛋白チロシン残基のリン酸化が関与していることを報告してきた。今年度は口腔内好中球の、活性化に関わる因子について解析して以下の知見をえた。(1)健常人より末梢血好中球と口腔内好中球を単離し、NF-kBのp55とp65そしてIkBの発現をウエスタンブロッテングで解析した結果、いずれの好中球も発現が認められた。(2)転写活性をゲルシフトアッセイで解析した結果、無刺激では、末梢血好中球には活性が認められないが、ホーボルエステルなどで刺激すると活性化された。一方、口腔内好中球は、無刺激でも、強い活性が認められた。(3)蛍光Differential Display法を用いて、口腔内好中球に特異的に発現しているmRNAを解析したところ、数種のタンパク質の発現が見られた。これらのmRNAのうち、もっとも強く発現していたものを塩基配列を解析した結果、PAC418A9と70%のホモロジーの、未知のタンパク質である可能性が示唆された。(5)口腔内好中球は、自発性にミエロペルオキシダーゼとNOを放出するため、電気化学検出器を用いたHPLCによる解析の結果、ニトロチロシンよりもむしろクロロチロシンが生成される傾向が示されつつある。

Research Products

(12 results)

All Other

All Publications (12 results)

[Publications] Nishikawa,M.,Sato,E.F.,Kuroki,T..Utsumi.K..and Inoue.M.: "Role of Glutachione in the energy metabolism of tumor cells." Hepatology. 27. 422-426 (1998)
[Publications] Nakanishi,K.,Nishi,Y.,Sato,E.F..Ishii.M..Hamada.T..Inoue.: "Termal injury induces thymocyte apoptosis in the rat." J.Trauma. 44・1. 143-148 (1998)
[Publications] Nakahara,H,Sato,E.F.,Ishihara,R..Kanno.T..Yoshioka.T..: "Biochemical properties of human oral polymorphonuclear leukocytes." Free Radic.Res.28. 485-495 (1998)
[Publications] Nishikawa,M.,Takeda,K.,Sato,E.F..Kuroki.T..Inoue.M.: "Nitric oxide regulates energy metabolism and bcl-2 expression in rat intestional epithelial cells" Am.J.Physiol.37・5. G797-801 (1998)
[Publications] Morishita,S.,Sato.E.F..Takahashi.P.Y..Manabe.M.and: "Hypoglycemia-induced apoptosis in rat thymocytes in vivo." Dyabetes Reserch and Clinical Practice. 40. 1-7 (1998)
[Publications] Yamamoto,H.,Sasaki,J.,Matsuura.J..Nomura.T..Sato.: "Caracterization of the inner enamel epithelium in the enamel-free area based on the ability to secrete enamel protein demonstrated by in situ hybridization and immunohistochemistry" Acta Anatomica. 160. 232-238 (1998)
[Publications] Nagata,K.,Yu,H.,Nishikawa,M.,Kashiba.M..Sato.E.F..Tamura.: "Irreversible inhibition of respiration of Helicobacter pylori by nitric oxide" J.Biol.Chem.273・23. 14071-14073 (1998)
[Publications] Minaniyama,Y.,Takemura,S.,Tovokuni.S..Tanimoto.Y..Sato.: "Processed gain food inhibits hepatic injury in endotoxemic rats" J.Nutr.Sci.Vitaminol.31・4. 275-279 (1998)
[Publications] Sasaki,J.,Yamamoto,H.,Nomura,T..Matsuura.J..Seno.M..Sato.: "Multiple-labeling of oligonucleotide probes for in situ hybridization" Acta Histochem.Cytochem.31・4. 275-279 (1998)
[Publications] Nishikawa,M.,Sato.E.F.,Kuroki,T..Utsumi.K..Inoue.M.: "Macrophage-derived Nitric oxide induces apoptosis of ascites hepatoma cells in vivo" Hepatology. 28・6. 1474-1480 (1998)
[Publications] Nishikawa,M.,Sato,E.F.,Utsumi,K.,Inoue,M.: "Biological Oxidants and Antioxidants:Molecular Mechanisms and" AOCS PRESS, 54-64 (1998)
[Publications] Utsumi,K.,Takehara,Y.,Inai,Y.,Yabuki,M.,Kanno,T.,Nishikawa,M.,Sato,EF.,Inoue,M.: "Mitochondria,Oxidants,and Aging" Academic Press, 57-72 (1999)

1998 Fiscal Year Annual Research Report

病態局所での好中球の活性化機構とシグナル伝達

Principal Investigator

佐藤 英介 大阪市立大学, 医学部, 助手 (60211942)

Research Products

[Publications] Nishikawa,M.,Sato,E.F.,Kuroki,T..Utsumi.K..and Inoue.M.: "Role of Glutachione in the energy metabolism of tumor cells." Hepatology. 27. 422-426 (1998)

[Publications] Nakanishi,K.,Nishi,Y.,Sato,E.F..Ishii.M..Hamada.T..Inoue.: "Termal injury induces thymocyte apoptosis in the rat." J.Trauma. 44・1. 143-148 (1998)

[Publications] Nakahara,H,Sato,E.F.,Ishihara,R..Kanno.T..Yoshioka.T..: "Biochemical properties of human oral polymorphonuclear leukocytes." Free Radic.Res.28. 485-495 (1998)

[Publications] Nishikawa,M.,Takeda,K.,Sato,E.F..Kuroki.T..Inoue.M.: "Nitric oxide regulates energy metabolism and bcl-2 expression in rat intestional epithelial cells" Am.J.Physiol.37・5. G797-801 (1998)

[Publications] Morishita,S.,Sato.E.F..Takahashi.P.Y..Manabe.M.and: "Hypoglycemia-induced apoptosis in rat thymocytes in vivo." Dyabetes Reserch and Clinical Practice. 40. 1-7 (1998)

[Publications] Yamamoto,H.,Sasaki,J.,Matsuura.J..Nomura.T..Sato.: "Caracterization of the inner enamel epithelium in the enamel-free area based on the ability to secrete enamel protein demonstrated by in situ hybridization and immunohistochemistry" Acta Anatomica. 160. 232-238 (1998)

[Publications] Nagata,K.,Yu,H.,Nishikawa,M.,Kashiba.M..Sato.E.F..Tamura.: "Irreversible inhibition of respiration of Helicobacter pylori by nitric oxide" J.Biol.Chem.273・23. 14071-14073 (1998)

[Publications] Minaniyama,Y.,Takemura,S.,Tovokuni.S..Tanimoto.Y..Sato.: "Processed gain food inhibits hepatic injury in endotoxemic rats" J.Nutr.Sci.Vitaminol.31・4. 275-279 (1998)

[Publications] Sasaki,J.,Yamamoto,H.,Nomura,T..Matsuura.J..Seno.M..Sato.: "Multiple-labeling of oligonucleotide probes for in situ hybridization" Acta Histochem.Cytochem.31・4. 275-279 (1998)

[Publications] Nishikawa,M.,Sato.E.F.,Kuroki,T..Utsumi.K..Inoue.M.: "Macrophage-derived Nitric oxide induces apoptosis of ascites hepatoma cells in vivo" Hepatology. 28・6. 1474-1480 (1998)

[Publications] Nishikawa,M.,Sato,E.F.,Utsumi,K.,Inoue,M.: "Biological Oxidants and Antioxidants:Molecular Mechanisms and" AOCS PRESS, 54-64 (1998)

[Publications] Utsumi,K.,Takehara,Y.,Inai,Y.,Yabuki,M.,Kanno,T.,Nishikawa,M.,Sato,EF.,Inoue,M.: "Mitochondria,Oxidants,and Aging" Academic Press, 57-72 (1999)

佐藤英介大阪市立大学, 医学部, 助手 (60211942)