1997 Fiscal Year Annual Research Report

骨組織由来腫瘍細胞誘引因子の単離・同定-ラット骨肉腫細胞株を用いて

Research Project

Project/Area Number	09770157
Research Institution	Yokohama City University
Principal Investigator	長嶋洋治横浜市立大学, 医学部, 講師 (10217995)
Keywords	転移 / 骨組織 / 骨芽細胞 / 骨肉腫 / SPARC / Osteonectin / BM40 / 遊走促進能 / IV型コラーゲン / 浸潤
Research Abstract	我々は平成9年度間に、骨形成能を有する細胞に由来する腎細胞癌細胞誘引物質の単離同定を、以下のように行った。骨形成性ラット骨肉腫培養細胞株YROS-1を無血清培地中で培養し、培養上清を濃縮、Boyden chamber assayにて腎細胞癌細胞株3株の遊走促進活性を検討した。遊走能を促進する活性を有する分画を各種カラムを用いて分離した。得られた分画はCoumassie blue染色にて還元条件下で40kD,非還元状態で35kDの単一バンドを得た。分子量ならびに骨芽細胞系由来であることを鑑みて、SPARC/Osteonectin/BM-40が活性物質であると仮定した。ウエスタンプロットにて同バンドは、抗SPARC抗体と反応性を示した。抗SPARC抗体を処理することによって遊走促進活性は失われた。さらに、Boyden chamberのコート基質をtype IV collagenから、fibronectin,laminin,などに変えたところ、遊走促進能は低下を示した。 SPARCは骨のみでなく、血管内皮細胞、血小板に広く存在していることから、骨に限らず、血管壁に対する腫瘍細胞の遊走能を促進させている可能性が示唆された。さらに、多くの腫瘍細胞株でSPARCの産生が見られており、腫瘍細胞が分泌するSPARCが、type IV collagenと結合することによって自身の浸潤を促進している可能性も示唆された。今後は、生体内での研究が必要と思われ、現在SPARC欠失マウスを用いての検討を計画中である。

Research Products
(19 results)

All Other

All Publications (19 results)

[Publications] Nagashima Y et, al.: "Expression of matrilysin in vascular endothelial cells adjacent to matrilysin-producing tumors." Int J Cancer. 72. 441-445 (1997)
[Publications] Kawano N, Nagashima Y et, al.: "Expression of gelatinase A, TIMP-2, matrilysin and trypsin (ogen) in lung neoplasms." Hum Pathol. 28. 613-622 (1997)
[Publications] Hidai H, Nagashima Y, et al.: "Bilateral chromophobe renal carcinoma in tuberous sclerosis complex." Int J Urol. 4. 86-89 (1997)
[Publications] Gorai I, Nagashima Y, et al.: "Uterine carcinosarcoma is derived from a single cell An in vitro study." Int J Cancer. 72. 821-827 (1997)
[Publications] Koshikawa N, Nagashima Y, et al.: "Expression of trypsin vascular endothelial cells." FEBS Lett. 409. 442-448 (1997)
[Publications] Nagashima Y, et al.: "Adult ganglioneuroblastoma of the anterior mediastinum." Pathol Res Pract. 193. 727-732 (1997)
[Publications] Takeuchi N, Nagashima Y, et al.: "Matrilysin gene expression in sporadic and familial colorectal. adenomas." Mol Carcinogenesis. 19. 225-229 (1997)
[Publications] Ishizuka S, Nagashima Y, et al.: "Regulation and immunohistochemical analysis of stress protein HO-1 in rat kidney with myoglobulinuric ARF." Biochem Biophys Res Comm. 240. 93-98 (1997)
[Publications] Udagawa K, Nagashima Y, et al.: "Specific expression of pp5/TFPI-2 mRNA by syncytiotrophoblasts in human placenta as revealed by ISH." Placenta. (in press).
[Publications] Nakatani Y, Nagashima Y, et al.: "Pulmonary adenocarcinoma of fetal lung type : A clinstudy indicating differrence." Am J Surg Pathol. (in press).
[Publications] Kato Y, Nagashima Y, et al.: "Production of trypsin by human gastric cancer cells correlates with their malignant phenotypes." Eur J Cancer. (in press).
[Publications] Ishizuka S, Nagashima Y, et al.: "Theophylline protects against glycerol-induced ARF Regulation of HO-1 and glutathione in rat kidney." Clin Exp Nephrol. 1. 204-211 (1997)
[Publications] Numata M, Nagashima Y, et al.: "Inhibition of inducible NOS prevents LPS-induced acute lung injury in dogs." J Immunol. (in press).
[Publications] Koshikawa N, Nagashima Y, et al.: "Expession of trypsin by endothelial cells of various tissues, leukocytes and neurons in mouse and human." Am J Pathol. (in press).
[Publications] Momiyama N, Nagashima Y, et al.: "Inhibitory effect of matrilysin antisense oligonucleotides on human colon cancer invasion in vitro." Int J Cancer. (in press).
[Publications] 神座慎一郎, 長嶋洋治, 他: "浸潤性発育を示したBellini管癌の1例" 泌尿紀要. 43. 291-294 (1997)
[Publications] 長嶋洋治, 他: "腎病変とvon Hippel-Lindau病" 病理と臨床. 15. 124-128 (1997)
[Publications] 宮崎香、長嶋洋治他: "大腸癌の現在と未来-遺伝子診断の臨床形態学への応用-" メジカルビュー社(印刷中),
[Publications] 長嶋洋治: "病理学実践マニュアル" 医学書院(印刷中),

1997 Fiscal Year Annual Research Report

骨組織由来腫瘍細胞誘引因子の単離・同定-ラット骨肉腫細胞株を用いて

Principal Investigator

長嶋 洋治 横浜市立大学, 医学部, 講師 (10217995)

Research Products

[Publications] Nagashima Y et, al.: "Expression of matrilysin in vascular endothelial cells adjacent to matrilysin-producing tumors." Int J Cancer. 72. 441-445 (1997)

[Publications] Kawano N, Nagashima Y et, al.: "Expression of gelatinase A, TIMP-2, matrilysin and trypsin (ogen) in lung neoplasms." Hum Pathol. 28. 613-622 (1997)

[Publications] Hidai H, Nagashima Y, et al.: "Bilateral chromophobe renal carcinoma in tuberous sclerosis complex." Int J Urol. 4. 86-89 (1997)

[Publications] Gorai I, Nagashima Y, et al.: "Uterine carcinosarcoma is derived from a single cell An in vitro study." Int J Cancer. 72. 821-827 (1997)

[Publications] Koshikawa N, Nagashima Y, et al.: "Expression of trypsin vascular endothelial cells." FEBS Lett. 409. 442-448 (1997)

[Publications] Nagashima Y, et al.: "Adult ganglioneuroblastoma of the anterior mediastinum." Pathol Res Pract. 193. 727-732 (1997)

[Publications] Takeuchi N, Nagashima Y, et al.: "Matrilysin gene expression in sporadic and familial colorectal. adenomas." Mol Carcinogenesis. 19. 225-229 (1997)

[Publications] Ishizuka S, Nagashima Y, et al.: "Regulation and immunohistochemical analysis of stress protein HO-1 in rat kidney with myoglobulinuric ARF." Biochem Biophys Res Comm. 240. 93-98 (1997)

[Publications] Udagawa K, Nagashima Y, et al.: "Specific expression of pp5/TFPI-2 mRNA by syncytiotrophoblasts in human placenta as revealed by ISH." Placenta. (in press).

[Publications] Nakatani Y, Nagashima Y, et al.: "Pulmonary adenocarcinoma of fetal lung type : A clinstudy indicating differrence." Am J Surg Pathol. (in press).

[Publications] Kato Y, Nagashima Y, et al.: "Production of trypsin by human gastric cancer cells correlates with their malignant phenotypes." Eur J Cancer. (in press).

[Publications] Ishizuka S, Nagashima Y, et al.: "Theophylline protects against glycerol-induced ARF Regulation of HO-1 and glutathione in rat kidney." Clin Exp Nephrol. 1. 204-211 (1997)

[Publications] Numata M, Nagashima Y, et al.: "Inhibition of inducible NOS prevents LPS-induced acute lung injury in dogs." J Immunol. (in press).

[Publications] Koshikawa N, Nagashima Y, et al.: "Expession of trypsin by endothelial cells of various tissues, leukocytes and neurons in mouse and human." Am J Pathol. (in press).

[Publications] Momiyama N, Nagashima Y, et al.: "Inhibitory effect of matrilysin antisense oligonucleotides on human colon cancer invasion in vitro." Int J Cancer. (in press).

[Publications] 神座慎一郎, 長嶋洋治, 他: "浸潤性発育を示したBellini管癌の1例" 泌尿紀要. 43. 291-294 (1997)

[Publications] 長嶋洋治, 他: "腎病変とvon Hippel-Lindau病" 病理と臨床. 15. 124-128 (1997)

[Publications] 宮崎香、長嶋洋治他: "大腸癌の現在と未来-遺伝子診断の臨床形態学への応用-" メジカルビュー社(印刷中),

[Publications] 長嶋洋治: "病理学実践マニュアル" 医学書院(印刷中),

長嶋洋治横浜市立大学, 医学部, 講師 (10217995)