1998 Fiscal Year Annual Research Report

食餌性n-3系不飽和脂肪酸の生理・病態に及ぼす影響の基礎的研究

Research Project

Project/Area Number	09771991
Research Institution	Nagoya City University
Principal Investigator	渡辺志朗名古屋市立大学, 薬学部, 講師 (00222406)
Keywords	d-リル_2酸 / エイコサペンタエン酸 / ドコサヘキサエン酸 / マクロファージ / 血小板活性化因子 / インターロイキンlB / 四塩化炭素 / インスリン低抗性
Research Abstract	1. n-3系列不飽和脂肪酸であるα-リノレン酸、エイコサペンタエン酸、ドコサヘキサエン酸が脂質性ケミカルメディエーターやサイトカインの産生、病態に及ぼす影響の比較 1) ラットの肺胞および腹腔マクロファージのカルシウムイオノフオア刺激下でのPAF産生が、α-リノレン酸を多く含むシソ油を与えることにより、リノール酸を多く含むベニバナ油を与えるよりも少なくなることが分かった。 2) 脾臓細胞のリポポリサッカライド刺激下でのIL-1βのmRNA発現誘導が、ドコサヘキサエン酸エチルエステルを添加した飼料を与えることにより、飽和、一価不飽和脂肪酸を多く含む牛脂を添加飼料を与えるよりも低くなることが分かった。しかしながら、エイコサペンタエン酸エチルエステル添加食群では、IL-1βのmRNA発現誘導の有意な低下はみられなかった。 3) シソ油およびドコサヘキサエン酸を多く含む魚油を与えることによって、II型糖尿病自然発症モデルであるOLETFラットのインシュリン感受性が、牛脂およびベニバナ油添加食群に比べて高くなることが分かった。 2. n-3系脂肪酸の長期摂取が過酸化傷害に及ぼす影響 1) ドコサヘキサエン酸を多く含む魚油を添加した飼料を長期に渡って与えても、マウスにおける四塩化炭素誘導慢性肝障害の症状を悪化させず、チオバルビツール酸反応性物質(TBARS)やカルボニル化蛋白質として測定した、脂質過酸化の程度の上昇は観察されなかった。

Research Products
(9 results)

All Other

All Publications (9 results)

[Publications] Oh-hashi,K.: "Effect of replacing a high lindeate oil with a low linoleate, high α-linolenate oils as compared with supplementing EPA or DHA on reducing lipid mediator production in rat polymorpho nuclear leukocytes." Biol.Pharm.Bull.21. 558-564 (1998)
[Publications] Nabeshima,S.: "Development of glycosylated human interleukin-ld, neoglyco IL-ld, bycoupling with D-galactose monosaccharide : biological activities invitro." Glycoconjugate J.15. 69-74 (1998)
[Publications] Miyazaki,M.: "Early mortality effect of partially hydrogenated vegetable oils in stroke-prone spontaneously hypertensive rats (SHR-SP)" Nutr.Res.18. 1049-1056 (1998)
[Publications] Watanabe,S.: "Absence of relation between the expression of cyclooxygenase isoforms and the synthesis of prostaglandin E_2 in resident and thioglycollate-elicited macropheges in rats." Prostaglandins and Other Lipid Mediators. 56. 7-18 (1998)
[Publications] Yasuda,S.: "Effects of dietary fatty acid unsaturation and chronic carbon tetrachloride treatment on accumulation of oxidation products, α-tocopherol and liver injury in mice." Biol.Pharm.Bull.21. 1050-1056 (1998)
[Publications] Miyazaki,M.: "Free fatty acid fractions from sorce vegetable oils exhibited reduced survivel time-shortening activity in stroke-prone spontaneously hypertensive rats." Lipids. 33. 655-661 (1998)
[Publications] Sato,A.: "Protein carbonyl contents roughly reflect the unsaturation of lipids in muscle but not in other tissues of SHRSP rats fed different fat and oils." Biol.Pharm.Bull.21. 1271-1276 (1998)
[Publications] Yasuda,S: "Dietary docosahexa enoic acid enhances ferric nitrilotriacetate-induced oxidative damage in mice but not when additional α-tocopherol is supblemented." Free Radic.Res.(in press).
[Publications] Matsuba,S.: "Effect of dietary linoleatel α-linolenate balance on experimentally induced gastric ulcers in rats." Prostaglandins Leukot.Essent,Fatty Acids.(in press).

1998 Fiscal Year Annual Research Report

食餌性n-3系不飽和脂肪酸の生理・病態に及ぼす影響の基礎的研究

Principal Investigator

渡辺 志朗 名古屋市立大学, 薬学部, 講師 (00222406)

Research Products

[Publications] Oh-hashi,K.: "Effect of replacing a high lindeate oil with a low linoleate, high α-linolenate oils as compared with supplementing EPA or DHA on reducing lipid mediator production in rat polymorpho nuclear leukocytes." Biol.Pharm.Bull.21. 558-564 (1998)

[Publications] Nabeshima,S.: "Development of glycosylated human interleukin-ld, neoglyco IL-ld, bycoupling with D-galactose monosaccharide : biological activities invitro." Glycoconjugate J.15. 69-74 (1998)

[Publications] Miyazaki,M.: "Early mortality effect of partially hydrogenated vegetable oils in stroke-prone spontaneously hypertensive rats (SHR-SP)" Nutr.Res.18. 1049-1056 (1998)

[Publications] Watanabe,S.: "Absence of relation between the expression of cyclooxygenase isoforms and the synthesis of prostaglandin E_2 in resident and thioglycollate-elicited macropheges in rats." Prostaglandins and Other Lipid Mediators. 56. 7-18 (1998)

[Publications] Yasuda,S.: "Effects of dietary fatty acid unsaturation and chronic carbon tetrachloride treatment on accumulation of oxidation products, α-tocopherol and liver injury in mice." Biol.Pharm.Bull.21. 1050-1056 (1998)

[Publications] Miyazaki,M.: "Free fatty acid fractions from sorce vegetable oils exhibited reduced survivel time-shortening activity in stroke-prone spontaneously hypertensive rats." Lipids. 33. 655-661 (1998)

[Publications] Sato,A.: "Protein carbonyl contents roughly reflect the unsaturation of lipids in muscle but not in other tissues of SHRSP rats fed different fat and oils." Biol.Pharm.Bull.21. 1271-1276 (1998)

[Publications] Yasuda,S: "Dietary docosahexa enoic acid enhances ferric nitrilotriacetate-induced oxidative damage in mice but not when additional α-tocopherol is supblemented." Free Radic.Res.(in press).

[Publications] Matsuba,S.: "Effect of dietary linoleatel α-linolenate balance on experimentally induced gastric ulcers in rats." Prostaglandins Leukot.Essent,Fatty Acids.(in press).

渡辺志朗名古屋市立大学, 薬学部, 講師 (00222406)