1998 Fiscal Year Final Research Report Summary
Origin and Evolution of the Vertebrate Immune System
| Project/Area Number |
09839029
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
自然史科学
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| Research Institution | The University of Tokyo (1998)
Nagoya City University (1997) |
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Principal Investigator |
NONAKA Masaru University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (40115259)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Complement / MHC / Innate Immunity / Ascidian / Medaka |
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| Research Abstract |
1) Molecular cloning and structural analysis of ascidian complement components :
cDNA clones for complement component MASP and C3 were isolated from the liver library of Japanese ascidian, Halocynthia roretzi. The deduced primary structures of these component indicated that they are ascidian counterparts of vertebrate MASP and C3. Antibodies against ascidian C3 inhibited an opsonic activity of ascidian body fluid to enhance phagocytosis of yeast by ascidian coelomocytes. These results indicate that the multi-component, opsonic complement system has been established by the time of urochordates emergence, much earlier than adaptive immunity.
2) Linkage analysis of mammalian MHC-encoded genes in Xenopus and Medaka fish :
Most mammalian MHC-encoded genes tested linked to each other in Xenopus, indicating the conservation of this linkage group between amphibians and mammals. In contrast, MHC class I, II and III genes showed a dispersed distribution all over Medaka fish chromosomes, except for a close linkage among the class IA, LMP2, LMP7 and TAP2 genes. Thus the class IA and its antigen processing/transport genes seem to be the core part of the MHC, indicating that the original physiological meaning of the MHC was to establish the class I antigen presentation system.
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[Publications] Endo, Y., Takahashi, M., Nakao, M., Saiga, H., Sekine, H., Matsushita, M., Nonaka, M.& Fujita, T.: "Two lineages of mannose binding lectin-associated serine protease (MASP) in vertebrate." Immunol.161. 4924-4930 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nonaka, M., Azumi, K., Ji, X., Namikawa-yamada, C., Sasaki, M., Saiga, H., Dodds, A.W., Sekine, H., Homma, M.K., Matsushita, M., Endo, Y.& Fujita, T.: "Opsonic complement component C3 in the solitary ascidian, Halocynthia roretzi." J.Immunol.162. 387-391
Description
「研究成果報告書概要(欧文)」より
