1998 Fiscal Year Annual Research Report

渦鞭毛藻を用いた真核生物間の細胞内共生の実験的解析

Research Project

Project/Area Number	09874183
Research Institution	Yamaguchi University
Principal Investigator	三室守山口大学, 理学部, 教授 (40142004)
Keywords	渦鞭毛藻 / 細胞内共生 / レクチン反応性 / 細胞周期 / 糖鎖認識
Research Abstract	細胞内共生を見るために培養を行なった渦鞭毛藻Alexandorium tamarenseの培養が困難を極めた.したがって,渦鞭毛藻に関する解析は今回は行なうことができなかった. 細胞内共生の最初の過程は,異種の生物の相互認識に始まると考えられる.この過程で最も重要な機能を果たすのは,確証はないが他の細胞の例から類推して,糖鎖と考えるのが妥当である.そこで,細胞表面の糖鎖の特性を見るために,ラフィド藻のChattonella marinaを使って,その感受性を検討し,渦鞭毛藻への適用のための基礎データとした. 14時間明期,10時間暗期の条件で生育させた細胞に,糖鎖認識部位の異なる4種のレクチンを添加してその影響をヴィデオによる顕微鏡下での連続観察によって検討したところ,細胞の溶解,運動性の低下などが観察された.さらに詳細にみると,溶解する細胞の多くは分裂直後の細胞である事が判明した.レクチンにより細胞の形が変わる(楕円形から桿形へ)細胞は分裂後数時間を経たもの,まったく感受性が認められない細胞は栄養成長の状態にあることが判明した.またレクチンの種類から感受性に関与する糖鎖は,N-acetylglucosamineである可能性が高くなった.これらの結果は,細胞周期にしたがって細胞表面に存在する糖鎖の量が関連していることを示し,まったく予想をしなかった画期的な結果を得た. こうした観察に基づいて,今後は細胞周期と細胞表面の糖鎖の質的,量的変動を解析し,微細藻類の細胞表面の認識機構をさらに追及する予定である.

Research Products

(14 results)

All Other

All Publications (14 results)

[Publications] Mimuro,M.,et al.: "Multiple pathways of the excitation energy flow in the phtosynthetic pigment system of a cryptophyta, Cryoptomonas sp.(CR-1)." Phycological Research. 45. 155-164 (1998)
[Publications] Takaichi,S.and M.Mimuro: "Distribution and geometrical isomerism of neoxanthin in oxygenic phototrophs: 9′-cis,a sole molecular form." Plant & Cell Physiology. 39. 968-977 (1998)
[Publications] Oba,T.,et al.: "Supramolecular structure of the chlorophyll a′ aggregate,and the origin of diastreoselective separation of chlorophyll a and a′." Journal of Physical Chemistry B. 102. 7882-7889 (1998)
[Publications] Papageorgiou,G.C et al.: "Membrane lipid unsaturation modulaters light-induced and osmotically-induced shift in chlorophyll a fluorescence of Synechocystis sp.PCC 6803." Photosynthesis Research. in press
[Publications] Mimuro,M.,et al.: "Fluorescence properties of Chlorophyll d-dominating prokaryotic alga,Acaryochloris marina: Studies by means of the time-resolved spectroscopy." Biochimica et Biophysica Acta. (in press).
[Publications] 三室守,秋本誠志: "カロテノイドの励起状態ダイナミクス-生体系における役割の決定因子" 光化学. 29. 6-13 (1998)
[Publications] M.Mimuro et al.: "Relaxation processes from the excited states and physiological function of carotenoids: Comparison of two molecular forms of neoxanrhin." Photomedicine and Photobiology. (in press).
[Publications] M.Mimuro et al.: "Fluoresce properties of chlorophyll d-dominating prokaryotic alga,Acaryochloris marina." Proceedings of the 11th Intenational Congres on Photosynthesis. (in press).
[Publications] H.Kikuch et al.: "Functional meaning of the symmetry structure in phycobiliproteins aggregates." Proceedings of the 11th Intenational Congres on Photosynthesis. (in press).
[Publications] S.Takaichi and M.Mimuro: "Distribution of 9′-cis neoxanthin in oxygenic phototrophs: A systematic study." Proceedings of the 11th Intenational Congres on Photosynthesis. (in press).
[Publications] G.C.Papageorgiou et al.: "Temperature and lipid unsaturation effects on plasma membranse(PM)and thylakoid membranes(TH)of Synechocystis sp.PCC6803." Proceedings of the 11th Intenational Congres on Photosynthesis. (in press).
[Publications] Mimuro,M.,H.Kikuchi and A.Murakami: "Photosynthesis and Plant Photobiology(in press)" Academic Press,London,
[Publications] 三室守: "多様性シリーズ藻類の多様性(in press)" 裳華房,
[Publications] 三室守: "光がもたらす生命と地球の共進化:エネルギーと食糧と地球環境に対するグローバルな視点(印刷中)" 弘文社出版,

1998 Fiscal Year Annual Research Report

渦鞭毛藻を用いた真核生物間の細胞内共生の実験的解析

Principal Investigator

三室 守 山口大学, 理学部, 教授 (40142004)

Research Products

[Publications] Mimuro,M.,et al.: "Multiple pathways of the excitation energy flow in the phtosynthetic pigment system of a cryptophyta, Cryoptomonas sp.(CR-1)." Phycological Research. 45. 155-164 (1998)

[Publications] Takaichi,S.and M.Mimuro: "Distribution and geometrical isomerism of neoxanthin in oxygenic phototrophs: 9′-cis,a sole molecular form." Plant & Cell Physiology. 39. 968-977 (1998)

[Publications] Oba,T.,et al.: "Supramolecular structure of the chlorophyll a′ aggregate,and the origin of diastreoselective separation of chlorophyll a and a′." Journal of Physical Chemistry B. 102. 7882-7889 (1998)

[Publications] Papageorgiou,G.C et al.: "Membrane lipid unsaturation modulaters light-induced and osmotically-induced shift in chlorophyll a fluorescence of Synechocystis sp.PCC 6803." Photosynthesis Research. in press

[Publications] Mimuro,M.,et al.: "Fluorescence properties of Chlorophyll d-dominating prokaryotic alga,Acaryochloris marina: Studies by means of the time-resolved spectroscopy." Biochimica et Biophysica Acta. (in press).

[Publications] 三室 守,秋本誠志: "カロテノイドの励起状態ダイナミクス-生体系における役割の決定因子" 光化学. 29. 6-13 (1998)

[Publications] M.Mimuro et al.: "Relaxation processes from the excited states and physiological function of carotenoids: Comparison of two molecular forms of neoxanrhin." Photomedicine and Photobiology. (in press).

[Publications] M.Mimuro et al.: "Fluoresce properties of chlorophyll d-dominating prokaryotic alga,Acaryochloris marina." Proceedings of the 11th Intenational Congres on Photosynthesis. (in press).

[Publications] H.Kikuch et al.: "Functional meaning of the symmetry structure in phycobiliproteins aggregates." Proceedings of the 11th Intenational Congres on Photosynthesis. (in press).

[Publications] S.Takaichi and M.Mimuro: "Distribution of 9′-cis neoxanthin in oxygenic phototrophs: A systematic study." Proceedings of the 11th Intenational Congres on Photosynthesis. (in press).

[Publications] G.C.Papageorgiou et al.: "Temperature and lipid unsaturation effects on plasma membranse(PM)and thylakoid membranes(TH)of Synechocystis sp.PCC6803." Proceedings of the 11th Intenational Congres on Photosynthesis. (in press).

[Publications] Mimuro,M.,H.Kikuchi and A.Murakami: "Photosynthesis and Plant Photobiology(in press)" Academic Press,London,

[Publications] 三室 守: "多様性シリーズ 藻類の多様性(in press)" 裳華房,

[Publications] 三室 守: "光がもたらす生命と地球の共進化:エネルギーと食糧と地球環境に対するグローバルな視点(印刷中)" 弘文社出版,

三室守山口大学, 理学部, 教授 (40142004)

[Publications] 三室守,秋本誠志: "カロテノイドの励起状態ダイナミクス-生体系における役割の決定因子" 光化学. 29. 6-13 (1998)

[Publications] 三室守: "多様性シリーズ藻類の多様性(in press)" 裳華房,

[Publications] 三室守: "光がもたらす生命と地球の共進化:エネルギーと食糧と地球環境に対するグローバルな視点(印刷中)" 弘文社出版,