1998 Fiscal Year Annual Research Report

ヒトTNF遺伝子を導入したヒト線維芽細胞の抗腫瘍効果

Research Project

Project/Area Number	09877045
Research Institution	Okayama University
Principal Investigator	難波正義岡山大学, 医学部, 教授 (80069004)
Co-Investigator(Kenkyū-buntansha)	井上裕介岡山大学, 医学部, 助手 (90304302) 辻俊也岡山大学, 医学部, 助手 (10304304)
Keywords	TNF / 癌治療 / TNF産生ヒト細胞 / カプセル化
Research Abstract	コバルト60ガンマー線照射で不死化した脱瘍性をもたないヒト線維芽細胞にTNF-αを導入し,TNF-αを産生する細胞のクローンを取得した.この細胞を培養した培地(Conditioned medium:CM)を,ヒト由来の肺癌(A549),卵巣癌(PA-1),肝癌(HLE,HLF,JHH-7),試験管内で癌化したヒト線維芽細胞(KMST-6/RAS)の培養培地に終濃度10%添加すると,これらの細胞の増殖を45-92%抑制した.この細胞増殖抑制作用はCMの濃度依存性であった.また,この細胞増殖抑制作用は細胞の産生ずるTNF-αであることを,抗TNF-α抗体で証明した.一方,ヒト由来の正常線維芽細胞(KMS-6),子宮癌(HeLa),肝癌(PLC/PLF/5,JI-II-I-l,HuH-6,HepG2)などの細胞の増殖は抑制しなかった.したがって,TNF-α産生細胞は約50%のヒト腫瘍の増殖を抑えることが分かった. このTNF-α産生細胞を,臨床的に応用できるかどうかを検討するために,この細胞をスフエロイド培養し,そのスフエロイドをアガロース,カルボキシメチール-レルローズでカプセル化した.このカプセル化TNF細胞は培地に1カ月間にわたってTNFを産生した.

Research Products
(6 results)

All Other

All Publications (6 results)

[Publications] Fushimi,K.: "Establishment of a human fibroblast cell line producing tumor necrosis factor α (KMST6/TNF) and growth inhibitory effects of its conditioned medium on malignant cells in culture." In Vitro Cell Dev Biol. 34. 463-467 (1998)
[Publications] Nakamura,K.: "Inhibitory effects of antioxidants on neonatal rat cardiac myocyte hypertrophy induced by tumor necrosis factor-α and angiotensin II." Circulation. 98. 794-799 (1998)
[Publications] Tsuji T.: "Cyclin E overexpression responsible for growth of human hepatic tumors with p21^<WAF1/CIP1/SDI1>" Biochem.Biophys.Res.Commun.242 (2). 317-321 (1998)
[Publications] Miyazaki,M.: "Transforming growth factor-β1 stimulates or inhibits cell growth via down-or up-regulation of p21/Waf1." Biochem.Biophys.Res.Commun.246. 873-880 (1998)
[Publications] Tagashira,H.: "Reduced metastatic potential and c-myc overexpression of colon adenocarcinoma cells (Colon 26 line) transfected with nm23-R2/rat nucleoside diphosphate kinase α isoform." Int.J.Mol.Med,. 2. 25-68 (1998)
[Publications] Kondo,T.: "Characteristics of intracellular transferrin produced by human fibroblasts : Its posttranscriptional regulation and association with tubulin." Exp.Cell Res.242. 38-44 (1998)

1998 Fiscal Year Annual Research Report

ヒトTNF遺伝子を導入したヒト線維芽細胞の抗腫瘍効果

Principal Investigator

難波 正義 岡山大学, 医学部, 教授 (80069004)

Research Products

[Publications] Fushimi,K.: "Establishment of a human fibroblast cell line producing tumor necrosis factor α (KMST6/TNF) and growth inhibitory effects of its conditioned medium on malignant cells in culture." In Vitro Cell Dev Biol. 34. 463-467 (1998)

[Publications] Nakamura,K.: "Inhibitory effects of antioxidants on neonatal rat cardiac myocyte hypertrophy induced by tumor necrosis factor-α and angiotensin II." Circulation. 98. 794-799 (1998)

[Publications] Tsuji T.: "Cyclin E overexpression responsible for growth of human hepatic tumors with p21^<WAF1/CIP1/SDI1>" Biochem.Biophys.Res.Commun.242 (2). 317-321 (1998)

[Publications] Miyazaki,M.: "Transforming growth factor-β1 stimulates or inhibits cell growth via down-or up-regulation of p21/Waf1." Biochem.Biophys.Res.Commun.246. 873-880 (1998)

[Publications] Tagashira,H.: "Reduced metastatic potential and c-myc overexpression of colon adenocarcinoma cells (Colon 26 line) transfected with nm23-R2/rat nucleoside diphosphate kinase α isoform." Int.J.Mol.Med,. 2. 25-68 (1998)

[Publications] Kondo,T.: "Characteristics of intracellular transferrin produced by human fibroblasts : Its posttranscriptional regulation and association with tubulin." Exp.Cell Res.242. 38-44 (1998)

難波正義岡山大学, 医学部, 教授 (80069004)