1997 Fiscal Year Annual Research Report

生体機能分子の設計・合成

Research Project

Project/Area Number	09NP0501
Research Institution	The University of Tokyo
Principal Investigator	輕部征夫東京大学, 先端科学技術研究センター, 教授 (50089827)
Co-Investigator(Kenkyū-buntansha)	鈴木正康九州工業大学, 情報工学部, 助教授 (70226554) 竹内俊文広島市立大学, 情報科学部, 教授 (70179612) 民谷栄一北陸先端科学技術大学院大学, 材料科学研究科, 教授 (60179893) 小宮山真東京大学, 大学院・工学系研究科, 教授 (50133096) 松永是東京農工大学, 工学部, 教授 (10134834)
Keywords	生体機能分子 / モレキュラーインプリンティング / 生体模倣技術 / 蛋白質工学 / 蛋白質の化学修飾 / エレクトロニクス素子 / バイオエレクトロニクス / バーチャルエボルーション
Research Abstract	本研究は、脳における情報処理や神経伝達機構などの、生物の持つ優れた機能を持ち、且つ工学的に応用可能な全く新しい「生体機能分子」の「設計・合成」を行うことを目的とする。生体機能分子を設計・合成するために、有機合成化学的アプローチと蛋白質化学的アプローチの2つの方針をとる。平成8年度までの段階で、有機合成的アプローチのモレキュラーインプリンティング法では、新規の分子認識機能を持った合成高分子が得られ、更にその分子認識機能の改良とセンサーへの応用の可能性が示された。蛋白質工学的アプローチにおいては、蛋白質を化学修飾しその電子伝達機能の改変を行った。更に同じく化学修飾を行うことにより、有機溶媒中で触媒機能をもつ酵素の作製にも成功している。平成9年度は、具体的に以下の2つを研究目的とした。 1.これまでに開発した生体機能分子の創製方法をまとめ、将来的な実用化の可能性を検討する。 2.コンピューター上の進化を利用して生体機能分子の設計を行う全く新しい手法「バーチャルエボルーション」の開発とこの手法を用いた新規生体機能分子の設計合成を試みる。これらの目的に対して以下の成果が得られた。 1.モレキュラーインプリンティング法により分子認識ポリマーを合成する方法及びその分子認識機能を向上させる方法を確立した。またそのセンサーへの応用の可能性も確認した。更に蛋白質と合成高分子や電子メディエーターをハイブリッドさせて電子伝達機能を改変することが出来た。 2.新規な生体機能分子の創製法である「バーチャルエボルーション」という手法の可能性を確認できた。

Research Products
(25 results)

All Other

All Publications (25 results)

[Publications] Kazuyoshi Yano,Kazunori Ikebukuro,Isao Karube et al.: "Development of a chemical vapor sensor using piezoelectric quartz crystals with coated unusual lipids." Analytica Chimica Acta. 340. 41-48 (1997)
[Publications] Koichi Sumikura,Kazuyoshi Yano,Isao Karube et al.: "Thrombin-binding properties of thrombin aptamer derivatives" Nucleic Acids Symposium Series. 37. 257-258 (1997)
[Publications] Teru Kato,Kazunori Ikebukuro and Isao Karube: "Selection of functional DNAs which bind to a transition state analog" Nucleic Acids Symposium Series. 37. 243-244 (1997)
[Publications] Raphael Levi,Scott McNiven,Soo-Hwan Cheong,Isao Karube et al.: "Optical Detection of Chloramphenicol Using Molecularly Imprinted Polymers" Analytical Chemistry. 69. 2017-2021 (1997)
[Publications] Satoshi Sasaki,Yoshiko Arikawa,Isao Karube et al.: "Measurement of Sulfite Using Sulfite Oxidase and Luminol Chemiluminescence" Analytical Communications. 34. 299-301 (1997)
[Publications] Hideaki Nakamura,Kazunori Ikebukuro,Isao Karube et al.: "A chemiluminescent FIA biosensor for phosphate ion monitoring using pyruvate oxidase" Biosensors & Bioelectronics. 12. 959-966 (1997)
[Publications] Yuko Amo and Isao Karube: "Dielectric measurements of Lysozyme and tri-N-acetyl-D-glucosamine association at radio and microwave" Biosensors & Bioelectronics. 12. 953-958 (1997)
[Publications] Runa Nakamura,Hitoshi Muguruma,Isao Karube et al.: "A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing" Analytical Chemistry. 69. 4649-4652 (1997)
[Publications] K.Yokoyama: "Catalytic electrochemistry of poly(ethylene glycol-modified galactose oxidase inorganic solvents" Denki Kagaku. 65. 477-478 (1997)
[Publications] K.Yokoyama: "Electrochemically mediated enzyme reaction of polyethyleneglycol-modified galactose oxidase in organic solvents" J.Electroanal.chem.434. 211-228 (1997)
[Publications] Y.Morita: "Cold-active enzymes from cold adapted bacteria" J.Amer、Oil Chem.Soc.74. 441-444 (1997)
[Publications] Y.Kuroda: "Self-organized prophyrin dimer as a highly specific receptor for pyrazine derivatives" J.Amer、Chem.Soc.119. 4929-4933 (1997)
[Publications] J.Sumaoka: "Homogeneous DNA hydrolysis by cerium(IV)/lanthanide(III)/dextran ternary complexes." Chem.Lett.1997. 507-508 (1997)
[Publications] K.Matsumura: "Enormously fast RNA hydrolysis by lanthanide(III)ions under physiological conditions.-Eminent candidates for novel tools of biotechnology-." J.Biochem.122. 387-394 (1997)
[Publications] J.Sumaoka: "Enzymatic manipulation of the fragments obtained cerium(IV)induced DNA scission:Characterization hydrolytic termini." Chem.Eur.J.4. 205-209 (1997)
[Publications] T.Fujiwara: "Multidimensional solid-state nuclear magentic resonance for correlating anisotropic interactions under magic-angle spinning conditions" J.Mag.Reson.124. 147- (1997)
[Publications] M.Yashiro: "Unique trinuclear Zinc(II)complex for efficient and selective RNA hydrolysis" J.Chem.Soc.,Chem.Commun.1997. 83-84 (1997)
[Publications] M.Endo: "Molecular design for a pinpoint RNA scission.Interposition of oligoamines between two DNA oligomers" J.Org.Chem.62. 846-852 (1997)
[Publications] M.Komiyama: "Kinetic analysis of diamine-catalyzed RNA hydrolysis" J.Org.Chem.62. 2155-2160 (1997)
[Publications] M.Komiyama: "DNA and RNA hydrolysis by metal complexes for artificial pin-point restriction enzymes" J.Inorg.Biochem.67. 341- (1997)
[Publications] A.Kugimiya: "Sialic acid-imprinted polymers using noncovalent interactions" Materials Sci.Eng.C. 4. 263-266 (1997)
[Publications] T.Matsunaga: "Drug delivery by magnetoliposomes containing bacterial magnetic particles" Cellular Eng.2. 7- (1997)
[Publications] T.Matsunaga: "Genetic analysis of magnetic bacteria" Materials Sci.Eng.C. 4. 287-289 (1997)
[Publications] T.Matsunaga: "Genetic analysis of biomagnetic crystal formation" J.Phys.IV France.
[Publications] Isao Karube: "Applied Biochemistry and Biotechnology" HUMANA PRESS, 30 (1997)

1997 Fiscal Year Annual Research Report

生体機能分子の設計・合成

Principal Investigator

輕部 征夫 東京大学, 先端科学技術研究センター, 教授 (50089827)

Research Products

[Publications] Kazuyoshi Yano,Kazunori Ikebukuro,Isao Karube et al.: "Development of a chemical vapor sensor using piezoelectric quartz crystals with coated unusual lipids." Analytica Chimica Acta. 340. 41-48 (1997)

[Publications] Koichi Sumikura,Kazuyoshi Yano,Isao Karube et al.: "Thrombin-binding properties of thrombin aptamer derivatives" Nucleic Acids Symposium Series. 37. 257-258 (1997)

[Publications] Teru Kato,Kazunori Ikebukuro and Isao Karube: "Selection of functional DNAs which bind to a transition state analog" Nucleic Acids Symposium Series. 37. 243-244 (1997)

[Publications] Raphael Levi,Scott McNiven,Soo-Hwan Cheong,Isao Karube et al.: "Optical Detection of Chloramphenicol Using Molecularly Imprinted Polymers" Analytical Chemistry. 69. 2017-2021 (1997)

[Publications] Satoshi Sasaki,Yoshiko Arikawa,Isao Karube et al.: "Measurement of Sulfite Using Sulfite Oxidase and Luminol Chemiluminescence" Analytical Communications. 34. 299-301 (1997)

[Publications] Hideaki Nakamura,Kazunori Ikebukuro,Isao Karube et al.: "A chemiluminescent FIA biosensor for phosphate ion monitoring using pyruvate oxidase" Biosensors & Bioelectronics. 12. 959-966 (1997)

[Publications] Yuko Amo and Isao Karube: "Dielectric measurements of Lysozyme and tri-N-acetyl-D-glucosamine association at radio and microwave" Biosensors & Bioelectronics. 12. 953-958 (1997)

[Publications] Runa Nakamura,Hitoshi Muguruma,Isao Karube et al.: "A Plasma-Polymerized Film for Surface Plasmon Resonance Immunosensing" Analytical Chemistry. 69. 4649-4652 (1997)

[Publications] K.Yokoyama: "Catalytic electrochemistry of poly(ethylene glycol-modified galactose oxidase inorganic solvents" Denki Kagaku. 65. 477-478 (1997)

[Publications] K.Yokoyama: "Electrochemically mediated enzyme reaction of polyethyleneglycol-modified galactose oxidase in organic solvents" J.Electroanal.chem.434. 211-228 (1997)

[Publications] Y.Morita: "Cold-active enzymes from cold adapted bacteria" J.Amer、Oil Chem.Soc.74. 441-444 (1997)

[Publications] Y.Kuroda: "Self-organized prophyrin dimer as a highly specific receptor for pyrazine derivatives" J.Amer、Chem.Soc.119. 4929-4933 (1997)

[Publications] J.Sumaoka: "Homogeneous DNA hydrolysis by cerium(IV)/lanthanide(III)/dextran ternary complexes." Chem.Lett.1997. 507-508 (1997)

[Publications] K.Matsumura: "Enormously fast RNA hydrolysis by lanthanide(III)ions under physiological conditions.-Eminent candidates for novel tools of biotechnology-." J.Biochem.122. 387-394 (1997)

[Publications] J.Sumaoka: "Enzymatic manipulation of the fragments obtained cerium(IV)induced DNA scission:Characterization hydrolytic termini." Chem.Eur.J.4. 205-209 (1997)

[Publications] T.Fujiwara: "Multidimensional solid-state nuclear magentic resonance for correlating anisotropic interactions under magic-angle spinning conditions" J.Mag.Reson.124. 147- (1997)

[Publications] M.Yashiro: "Unique trinuclear Zinc(II)complex for efficient and selective RNA hydrolysis" J.Chem.Soc.,Chem.Commun.1997. 83-84 (1997)

[Publications] M.Endo: "Molecular design for a pinpoint RNA scission.Interposition of oligoamines between two DNA oligomers" J.Org.Chem.62. 846-852 (1997)

[Publications] M.Komiyama: "Kinetic analysis of diamine-catalyzed RNA hydrolysis" J.Org.Chem.62. 2155-2160 (1997)

[Publications] M.Komiyama: "DNA and RNA hydrolysis by metal complexes for artificial pin-point restriction enzymes" J.Inorg.Biochem.67. 341- (1997)

[Publications] A.Kugimiya: "Sialic acid-imprinted polymers using noncovalent interactions" Materials Sci.Eng.C. 4. 263-266 (1997)

[Publications] T.Matsunaga: "Drug delivery by magnetoliposomes containing bacterial magnetic particles" Cellular Eng.2. 7- (1997)

[Publications] T.Matsunaga: "Genetic analysis of magnetic bacteria" Materials Sci.Eng.C. 4. 287-289 (1997)

[Publications] T.Matsunaga: "Genetic analysis of biomagnetic crystal formation" J.Phys.IV France.

[Publications] Isao Karube: "Applied Biochemistry and Biotechnology" HUMANA PRESS, 30 (1997)

輕部征夫東京大学, 先端科学技術研究センター, 教授 (50089827)